Microproteome of dentoalveolar tissues

Salmon, Cristiane R.; Giorgetti, Ana Paula Oliveira; Leme, Adriana Franco Paes; Domingues, Romênia R.; Kolli, Tamara N.; Foster, Brian L.; Nociti, Francisco Humberto

doi:10.1016/j.bone.2017.05.014

Cited by 32 publications

(31 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Samples were sonicated and centrifuged briefly. Whole protein extracts were reduced, alkylated, and trypsin digested as described previously [28–30]. The resulting tryptic peptide samples were dried in a vacuum concentrator and reconstituted in 0.1% formic acid for analysis.…”

Section: Methodsmentioning

confidence: 99%

Osteopontin regulates dentin and alveolar bone development and mineralization

Foster

Salmon

et al. 2018

Bone

107

View full text Add to dashboard Cite

The periodontal complex is essential for tooth attachment and function and includes the mineralized tissues, cementum and alveolar bone, separated by the unmineralized periodontal ligament (PDL). To gain insights into factors regulating cementum-PDL and bone-PDL borders and protecting against ectopic calcification within the PDL, we employed a proteomic approach to analyze PDL tissue from progressive ankylosis knock-out (Ank) mice, featuring reduced PP, rapid cementogenesis, and excessive acellular cementum. Using this approach, we identified the matrix protein osteopontin (Spp1/OPN) as an elevated factor of interest in Ank mouse molar PDL. We studied the role of OPN in dental and periodontal development and function. During tooth development in wild-type (WT) mice, Spp1 mRNA was transiently expressed by cementoblasts and strongly by alveolar bone osteoblasts. Developmental analysis from 14 to 240days postnatal (dpn) indicated normal histological structures in Spp1 comparable to WT control mice. Microcomputed tomography (micro-CT) analysis at 30 and 90dpn revealed significantly increased volumes and tissue mineral densities of Spp1 mouse dentin and alveolar bone, while pulp and PDL volumes were decreased and tissue densities were increased. However, acellular cementum growth was unaltered in Spp1 mice. Quantitative PCR of periodontal-derived mRNA failed to identify potential local compensators influencing cementum in Spp1 vs. WT mice at 26dpn. We genetically deleted Spp1 on the Ank mouse background to determine whether increased Spp1/OPN was regulating periodontal tissues when the PDL space is challenged by hypercementosis in Ank mice. Ank; Spp1 double deficient mice did not exhibit greater hypercementosis than that in Ank mice. Based on these data, we conclude that OPN has a non-redundant role regulating formation and mineralization of dentin and bone, influences tissue properties of PDL and pulp, but does not control acellular cementum apposition. These findings may inform therapies targeted at controlling soft tissue calcification.

show abstract

Section: Methodsmentioning

confidence: 99%

Osteopontin regulates dentin and alveolar bone development and mineralization

Foster

Salmon

et al. 2018

Bone

107

View full text Add to dashboard Cite

show abstract

“…8. Proteins common to bone and other biomineralization systems found in the inner ear of adult black bream (Acanthopagrus butcheri, n = 3) [11,12,49,50]. In addition to structural collagens and proteoglycans, we also found proteins that are associated with osteoblast function or otherwise act as substrates for extracellular matrix formation [52,75,76,81].…”

Section: Transcriptome Sequencing Assembly and Annotationmentioning

confidence: 85%

“…We found the presence of many other proteins involved in the biomineralization of bone, cartilage or teeth in other vertebrates (Fig. 8) [11,12,49,50]. This suggests that the macromolecules involved in biomineralization are highly conserveda given protein may carry out the same function in separate systems, regardless of whether the mineral is hydroxyapatite (bone, cartilage or teeth) or calcium carbonate (otolith).…”

Section: Otoliths Are Like Bones But Inertmentioning

confidence: 86%

See 1 more Smart Citation

The inner ear proteome of fish

et al. 2018

View full text Add to dashboard Cite

The mechanisms that underpin the formation, growth and composition of otoliths, the biomineralized stones in the inner ear of fish, are largely unknown, as only a few fish inner ear proteins have been reported. Using a partial transcriptome for the inner ear of black bream (Acanthopagrus butcheri), in conjunction with proteomic data, we discovered hundreds of previously unknown proteins in the otolith. This allowed us to develop hypotheses to explain the mechanisms of inorganic material supply and daily formation of growth bands. We further identified a likely protein mediator of crystal nucleation and an explanation for the apparent metabolic inertness of the otolith. Due to the formation of both daily and annual increments, otoliths are routinely employed as natural chronometers, being used for age and growth estimation, fisheries stock assessments, and the reconstruction of habitat use, movement, diet and the impacts of climate change. Our findings provide an unprecedented view of otolith molecular machinery, aiding in the interpretation of these essential archived data.

show abstract

“…LCM technology has been used in a wide variety of applications with focus on genomic, transcriptomic, and even proteomic analyses, such as dissection of polar bodies from oocytes for pre-fertilization genetic diagnosis [9], transcriptome-wide analysis of blood vessels from human skin and wound-edge tissue [10], proteomic profiling of dentoalveolar tissues [11], and many other areas [12–14]. But only one study so far used a combination of LCM and mass spectrometry (MS) to localize unique proteins, potential biomarkers, when dealing with the heterogeneity of breast tumor [15].…”

Section: Introductionmentioning

confidence: 99%

Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes ofEudiplozoon nipponicum(Monogenea, Polyopisthocotylea)

Roudnický

Potěšil²,

Zdráhal³

et al. 2020

Preprint

View full text Add to dashboard Cite

23Eudiplozoon nipponicum (Goto, 1891) is a hematophagous monogenean ectoparasite 24 which inhabits the gills of the common carp (Cyprinus carpio). Heavy infestation can lead to 25 anemia and in conjunction with secondary bacterial infections cause poor health and eventual 26 death of the host. 27 This study is based on an innovative approach to protein localization which has never 28 been used in parasitology before. Using laser capture microdissection, we dissected particular 29 areas of the parasite body without contaminating the samples by surrounding tissue, and in 30 combination with analysis by mass spectrometry obtained tissue-specific proteomes of 31 tegument, intestine, and parenchyma of our model organism, E. nipponicum. We successfully 32 verified the presence of certain functional proteins (e.g. cathepsin L) in tissues where their 33 presence was expected (intestine) and confirmed that there were no traces of these proteins in 34 other tissues (tegument and parenchyma). Additionally, we identified a total of 2,059 proteins, 35 including 72 peptidases and 33 peptidase inhibitors. As expected, the greatest variety was found 36 in the intestine and the lowest variety in the parenchyma. 37Our results are significant on two levels. Firstly, we demonstrated how one can localize 38 all proteins in one analysis and without using laboratory animals (antibodies for 39 immunolocalization of single proteins). Secondly, this study offers the first complex proteomic 40 data on not only the E. nipponicum but within the whole class of Monogenea, which was from 41 this point of view until recently neglected. 42 3 43 Introduction 44 Laser-capture microdissection (LCM) was developed as a powerful and reliable tool to 45 overcome the heterogeneity of the specimen [1]. It enables isolation of specific tissues, 46 individual cells, or even individual organelles from complex samples based on cell morphology 47 [2,3]. The method was first described in the second half of the 20 th century and since then further 48 developed and modified [2,4-7]. Nowadays, LCM combines laser excision with high-resolution 49 microscopic control, which makes it possible to precisely capture for instance individual cells 50 or even only nucleus while keeping track of the location and morphology of the source tissue 51 [2,3,8]. 52 LCM technology has been used in a wide variety of applications with focus on genomic, 53 transcriptomic, and even proteomic analyses, such as dissection of polar bodies from oocytes 54 for pre-fertilization genetic diagnosis [9], transcriptome-wide analysis of blood vessels from 55 human skin and wound-edge tissue [10], proteomic profiling of dentoalveolar tissues [11], and 56 many other areas [12-14]. But only one study so far used a combination of LCM and mass 57 spectrometry (MS) to localize unique proteins, potential biomarkers, when dealing with the 58 heterogeneity of breast tumor [15]. 59 LCM has also been used in parasitology, especially in sample preparation. Recent 60 studies employed this method in...

show abstract

Microproteome of dentoalveolar tissues

Cited by 32 publications

References 78 publications

Osteopontin regulates dentin and alveolar bone development and mineralization

Osteopontin regulates dentin and alveolar bone development and mineralization

The inner ear proteome of fish

Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes ofEudiplozoon nipponicum(Monogenea, Polyopisthocotylea)

Contact Info

Product

Resources

About