Micropropagules can Profitably Save the Choicest Silk Banana from Extinction

Waman, Ajit Arun; Bohra, Pooja; Sathyanarayana, B. N.; Gourish, R. K.; Ashok, T. H.

doi:10.1007/s40011-014-0349-0

Cited by 9 publications

(3 citation statements)

References 11 publications

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“…is not only enjoyed as a dessert fruit but also used as staple food in many parts of the developing world owing to its superior nutritional properties. Micropropagation has been one of the major multiplication methods in a number of banana varieties, mainly because the regenerants in most of the cases perform better than the conventional counterparts, assuring profit to the growers [1]. The technique has revolutionized the banana industry, making banana a prime crop in the global tissue culture industry [2].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Multiplication and Root Induction in Silk Banana Variety Nanjanagud Rasabale as Influenced by Monochromatic Light Spectra

Waman

Bohra

Sathyanarayana

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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A number of factors are known to influence the aseptic culture multiplication, rooting and subsequent hardening in banana. Study of these factors would not only improve the in vitro response but also help in increasing the understanding of its basis. During present investigation, in vitro culture proliferation and root induction response in Silk banana as affected by different monochromatic lights (red, white, green and blue) was studied. In multiplication stage, cultures were grown on media with or without supplemental cytokinins. Results revealed that white light promoted shoot multiplication and growth on cytokinin rich media, when compared with other lights studied. Shoot multiplication was also improved in red light as compared to blue and green, but noticeable reduction in the quality of shoots was reported. Incubation of shoots under blue lights improved the quality of the shoots, and the regenerants were uniform in growth. White and blue lights also supported root induction and growth related parameters during in vitro root induction phase. Considering the growth response and uniformity of the cultures, blue light could be effectively used as an option in the slow growth culture in banana, however further investigations could help in optimizing the culture conditions.

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Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Multiplication and Root Induction in Silk Banana Variety Nanjanagud Rasabale as Influenced by Monochromatic Light Spectra

Waman

Bohra

Sathyanarayana

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…To overcome this problem, tissue culture or in vitro propagation offers an alternative over traditional propagation methods facilitating, large scale production of disease-free planting materials all year round keeping, physiological uniformity of the plants from using only a few explants (Abeyaratne and Lathiff, 2002;Waman et al, 2014). Meristem culture offers an efficient method for rapid clonal propagation with production of virus free materials (Helliot et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Standardization of protocol for in vitro propagation of banana (Musa sapientum)

Hoque

Robbani

Hasan

et al. 2018

J Bangladesh Agric Univ

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An experiment was conducted at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University during the period from August 2016 to April 2017 to standardize the protocol for in vitro propagation of banana. The experiment was laid out in completely randomized design with four replications. Three to four months aged field grown rhizome attached shoots were used as explants and cultured on MS medium with different concentrations and combinations of BAP (6-benzylamino purine), BAP + KIN (Kinetin) + NAA (Naphthalene Acetic Acid) and IBA (Indole-3-Butyric Acid) + IAA (Indole-3-Acetic Acid) to observe their efficacy on single shoot initiation, shoot multiplication and root formation respectively. Minimum number of days required for shoot initiation (9.07) with highest shoot initiation percentage (91.14) and the longest shoot (2.23 cm) was found in MS medium supplemented with 5.0 mg/L BAP. On the other hand, highest shoot multiplication percentage (80.99) with maximum number of shoots per explant (4.47), the highest length of shoots (4.17 cm) and maximum number of leaves (4.04)was observed in MS medium supplemented with 4.0 mg/L BAP + 2.0 mg/L KIN + 2.0 mg/L NAA. In case of root regeneration, the best results on days required for root initiation (9.00), the highest root initiation percentage (85.05), maximum number of roots per plantlet (5.83) and the highest length of roots (4.17 cm) was obtained in MS medium supplemented with1.5 mg/L IBA + 0.5 mg/L IAA. After 5-7 days of hardening in room temperature, established plantlets were ready for planting.

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“…Tissue culture or in vitro propagation offers such alternative and large numbers of plants can be regenerated using only a few explants (Dadjo et al, 2014). In vitro propagation of bananas has advantages over traditional propagation methods, which include large scale production of disease-free planting materials all year round, physiological uniformity and the availability of disease-free material (Daniells and Smith, 1991;Abeyaratne and Lathiff, 2002;Waman et al, 2014). Furthermore, micro-propagated bananas plants, in general, give higher yields and have shorter production periods as compared to conventional propagated bananas (CIAT, 2006).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of two elite cooking banana cultivars- FHIA 17 and INJAGI

Kahia¹,

Ndaruhutse²,

Waweru³

et al. 2015

Int. J. Biotechnol. Mol. Biol. Res.

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Explants of two elite cooking banana cultivars, FHIA17 and INJAGI were collected from healthy source of stock plants growing in the field. Sterilization was evaluated using different concentrations (20, 30, 40, 50 and 60%) of a commercial bleach (JIK) for 25 min. Effects of cytokinins benzyl amino purine (BAP), 2-isopentenyl adenine (2iP) and kinetin each evaluated at 5, 10, 20, 24 and 40 µM and thidiazuron (TDZ) at 0.1, 0.5, 1 and 1.5 µM, on microshoot induction were investigated. Effects of auxins viz. napthaleneacetic acid (NAA), indole-3-acetic acid (IAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-butyric acid (IBA each evaluated at 5, 10, 20 and 40 µM) on rooting were tested as well. The highest (91%) numbers of clean explants were obtained when the explants were subjected to sterilization in 40% Jik for 25 min. The FHIA 17 explants cultured on MS media supplemented with 40 µM kinetin produced the highest (3.00±0.35) mean number of microshoots. On the other hand, INJAGI explants cultured on MS media supplemented with 0.1 µM TDZ yielded the highest mean number (1.84±0.24) of microshoots and the highest mean length (0.99±0.13). Rooting was achieved in all media supplemented with the auxins evaluated except on 2,4-D. These results have an important significance on the application of tissue culture in propagation of cooking bananas in Rwanda, which is highly desired to support the government policy of replacing old unproductive bananas with elite high yielding varieties.

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Micropropagules can Profitably Save the Choicest Silk Banana from Extinction

Cited by 9 publications

References 11 publications

In Vitro Shoot Multiplication and Root Induction in Silk Banana Variety Nanjanagud Rasabale as Influenced by Monochromatic Light Spectra

In Vitro Shoot Multiplication and Root Induction in Silk Banana Variety Nanjanagud Rasabale as Influenced by Monochromatic Light Spectra

Standardization of protocol for in vitro propagation of banana (Musa sapientum)

In vitro propagation of two elite cooking banana cultivars- FHIA 17 and INJAGI

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