Micropropagation studies inCeropegia SPP

Patil, Vishwanath M

doi:10.1007/bf02822714

Cited by 33 publications

(31 citation statements)

References 8 publications

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“…Among the cytokinins tested, KIN was less effective than BAP or TDZ for multiple shoot induction. The present results are i n agreement with previous reports on C. bulbosa and C. jainii revealed that the BAP alone can induce axillary shoot multiplication from nodal segments (Patil, 1998). On the other hand, a synergistic effect of a range of growth regulators in combination with BAP for shoot regeneration was well documented for members o f Asclepiadaceae viz., C. candelabrum (Beena et al, 2003), Holostemma ada kodien (Martin, 2002) and Hemidesmus indica (Sreekumar et al, 2000).…”

Section: Effect Of Bap Kn and Tdz On Shoot Regenerationsupporting

confidence: 93%

“…bulbosa and C. bulbosa var. lushii (Patil, 1998). On the contrary, in Ceropegia candelabrum (Beena and Martin, 2003); Decalepis hamiltonii (Giridhar et al, 2004) produced callus from the leaf and internodal explants.…”

mentioning

confidence: 99%

“…The 2, 4-D is the principle auxin to induce the callus, it was best observed in case of other taxa too, Ceropegia species viz., C. jainii, C. bulbosa var bulbosa and C. bulbosa var lushii (Patil, 1998); Gymnema sylvestris (Gopi and Vatsala, 2006;Roy et al, 2008). Whereas, the other auxin also used to induce the callus i n Tylophora indicia ( The 2, 4-D 0.45 µM LG had the ability to produce hairy 1 roots (99%).…”

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confidence: 99%

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Induction of Morphogenetic Callus and Multiple Shoot Regeneration in Ceropegia pusilla Wight and Arn.

Kondamudi¹,

Vijayalakshmi²,

Sri³

et al. 2010

Biotechnology

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This study was undertaken to evaluate the most suitable concentration of plant growth regulators and perfect explant (node, internode and thin cell layer explants-TCLs) for callus induction and subsequent organogenesis in an endangered medicinal Ceropegia pusilla. The best callus induction was found on the MS medium supplemented with 6-benzylaminopurine (BAP) 13.32 µM LG + 2, 4-dichlorophenoxy acetic acid (2,4-D) survived. The plantlets were established, acclimatized and thrived in green house conditions with 80%. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bio active constituents of this medicinal plant.

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Section: Effect Of Bap Kn and Tdz On Shoot Regenerationsupporting

confidence: 93%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Induction of Morphogenetic Callus and Multiple Shoot Regeneration in Ceropegia pusilla Wight and Arn.

Kondamudi¹,

Vijayalakshmi²,

Sri³

et al. 2010

Biotechnology

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“…Significant callus production from both the explants was obtained on MS containing in colour except on 2,4-D-containing medium, where the callus was white. Previous studies show that in C. jainii and C. bulbosa (Patil, 1998); the highest callus induction frequency from nodal segment occurred on MS medium supplemented with 9.05 μM 2,4-D. Similarly in Ceropegia candelabrum, optimum callus induction was noticed from leaf and internode explants grown on MS supplemented with 4.52 μM 2,4-D (Beena et al, 2003).…”

Section: Callus Culturesmentioning

confidence: 88%

Multiple shoot regeneration and alkaloid cerpegin accumulation in callus culture of Ceropegia juncea Roxb.

Nikam

Savant

2009

Physiol Mol Biol Plants

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This is the first report of in vitro propagation and alkaloid accumulation in callus cultures of Ceropegia juncea Roxb. a source of "Soma" drug in Ayurvedic medicine. Multiple shoots and callus induction was optimized by studying the influence of auxins [IAA (Indole-3-acetic acid), NAA (2-Naphthalene acetic acid) and 2,4-D (2,4-Dichlorophenoxyacetic acid.)] and cytokinins [BA (6-benzyladenine) and Kin (Kinetin)] alone and in combinations. The best response for multiple shoot induction was obtained in nodal explants on MS medium supplemented with 7.5 μM Kin (8.5 ± 3 shoots per explants). The shoots were rooted on half strength MS (Murashige and Skoog's) medium fortified with either IAA or NAA (0.5-2.0 μM). The plantlets were transferred directly to the field with 100 % success rate. Supplementation of MS medium with auxins and cytokinins enhanced the growth of callus but inhibited the shoot regeneration in nodal explants. Best callus induction and proliferation observed on MS + 1 μM 2,4-D+5 μM BA. However the maximum cerpegin content (470 μg/g dry weight) was recorded in dried callus derived on MS+10 μM IAA+5 μM BA. Quantitative TLC (Thin layer chromatography) studies of the callus revealed a phytochemical profile similar to that of naturally grown plants. The calli were maintained by subculturing at 4 weeks interval on fresh parent medium over a period of 34 months. The optimized in vitro propagation and callus culture protocol offers the possibilities of using organ/callus culture technique for vegetative propagation and production of cerpegin alkaloid.

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“…In vitro techniques used for conserving wild and endemic species of Ceropegia by mass multiplication for subsequent reintroduction in their natural habitat (Patil, 1998 …”

Section: Discussion and Concluctionmentioning

confidence: 99%

Micropropagation of traditional medicinal plant Ceropegia juncea

Binish¹

2018

Ann.Pl.Sci.

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Ceropegia species which possess wide medicinal properties are being used in different traditional medicinal systems that are used by tribal people for curing different ailments. Ceropegia juncea was reported to be the source of 'Soma', a plant drug of the Ayurvedic system of medicine. The plant extract is used for the treatment of antiinflammatory, analgesic, antiulcer activities, liver disorders, hypotension, ulcerative condition and fever. It is also used as typical anesthetic agent. The present study was conducted to establish a protocol for in-vitro propagation of an endemic medicinal plant Ceropegia juncea maximum shoot proliferation better shoots with a sprouting frequency of 86% and with an average of 8.28 ±1.11 shoots /explants and attained a length of 5.37±0.74 cm was achieved on Murashige and Skoog's, 1962 (MS) medium supplemented with 6-benzylaminopurine (BAP) 1.5 mg/L + NAA 1.0mg/L and highest rooting of in vitro derived shoots was achieved on half MS with IBA 0.75mg/L.

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Micropropagation studies inCeropegia SPP

Cited by 33 publications

References 8 publications

Induction of Morphogenetic Callus and Multiple Shoot Regeneration in Ceropegia pusilla Wight and Arn.

Induction of Morphogenetic Callus and Multiple Shoot Regeneration in Ceropegia pusilla Wight and Arn.

Multiple shoot regeneration and alkaloid cerpegin accumulation in callus culture of Ceropegia juncea Roxb.

Micropropagation of traditional medicinal plant Ceropegia juncea

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