Micropropagation of the pistachio and its rootstocks by temporary immersion system

Akdemir, Hülya; Süzerer, Veysel; Onay, Ahmet; Tilkat, Engin; Ersalı, Yusuf; Çiftçi, Yelda Özden

doi:10.1007/s11240-013-0421-0

Cited by 58 publications

(65 citation statements)

References 38 publications

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“…The results obtained for chestnut micropropagation in TIS confirm the findings reported for other trees such as eucalyptus, teak, and pistachio, which showed high proliferation rates when TIS were applied (McAlister et al 2005;Quiala et al 2012;Akdemir et al 2014). A further advantage of the use of TIS was the reduction of costs in media constituents, media preparation, and waste disposal, as a result of agar elimination, together with less manual labor in the flow cabinet since the inoculation of the explants into the bioreactors was faster than in smaller containers such as tubes or jars.…”

Section: Resultssupporting

confidence: 85%

“…Genotypic differences are frequent in conventional micropropagation of woody plants (Nehra et al 2005;Pijut et al 2011), including chestnut (Vieitez et al 2007). This also applies to plants cultured in TIS, as reported for eucalyptus (McAlister et al 2005), blueberry (Debnath 2009), and pistachio (Akdemir et al 2014), and also to the chestnut clones used in this study. Higher values of multiplication coefficients were obtained in seven of these clones cultured in TIS, although a significant difference relative to SS conditions was only observed in clone 90025.…”

Section: Resultsmentioning

confidence: 56%

“…Hyperhydric shoots have not been observed during the culture of other tree species in TIS, such as Crescentia cujete (Murch et al 2004) and willow (Vidal et al 2015). However, a certain level of hyperhydricity has been reported in many woody species cultured in TIS, such as apple (Chakrabarty et al 2003;Zhu et al 2005), eucalyptus (McAlister et al 2005), cloudberry (Rubus chamaemorus) and blueberry (Debnath 2007(Debnath , 2009), teak (Quiala et al 2012), walnut (Moreno et al 2012), and pistachio (Akdemir et al 2014). In these studies, hyperhydricity was controlled by adjusting the immersion frequency or by reducing the cytokinin concentration.…”

Section: Resultsmentioning

confidence: 99%

“…(pistachio) (Murch et al 2004;McAlister et al 2005;Chakrabarty et al 2003;Zhu et al 2005;Debnath 2009;Quiala et al 2012;Moreno et al 2012;Akdemir et al 2014). Troch et al (2010) performed several experiments to micropropagate axillary shoots of European chestnut in liquid medium using bioreactors of the twin flask system (Escalona et al 1999).…”

Section: Introductionmentioning

confidence: 99%

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A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five artificial hybrids and five natural hybrids of Asian and European chestnut selected for resistance to ink disease. The type of explant influenced shoot quality and proliferation rates, and basal explants with callus produced more and longer shoots than apical and nodal segments. Use of rockwool cubes as support material prevented hyperhydricity and allowed proliferation of explants in Murashige and Skoog medium with half-strength nitrates supplemented with 0.22 lM BA and 3 % sucrose, cultured both in plantform TM and RITA Ò vessels with three or six immersions per day and additional aeration of 1 min per hour in the case of plantform TM bioreactors. Basal explants cultured in plantform TM for 5 weeks produced long shoots suitable for rooting, whereas apical and nodal explants cultured in plantform TM or RITA Ò produced shorter shoots that were suitable for maintenance of stock. For most of the clones, similar or higher proliferation rates were observed when cultured in liquid medium than in semisolid medium, with the additional benefit of cost-reduction of the former system. Shoots developed in liquid medium were submitted to ex vitro root induction by dipping in indole-3-butyric acid, and acclimatized under greenhouse conditions. This is the first demonstration of the production of chestnut plantlets from shoots cultured in liquid medium, and the protocol presented here shows good potential for application in large-scale propagation.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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“…Many CIB plantlets were did not develop normally, and the results of in vitro conditions did not support the production of healthy plants. For this reason, many researchers have used the TIB system to increase the multiplication ratio and root initiation from in vitro plantlets (Yan et al 2011;Niemenak et al 2013;Akdemir et al 2014;Ramos-Castella et al 2014;Gao et al 2015). Relatively high humidity seemed to result in high stomatal density in the semi-solid medium.…”

Section: Discussionmentioning

confidence: 99%

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

An¹,

Kim²,

Moon³

et al. 2016

J Plant Biotechnol

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We compared germination efficiency for somatic embryos (SE) of Liriodendron tulipifera using semi-solid (SS), temporary immersion bioreactors (TIB), and continuous immersion bioreactors (CIB) to produce vigorous plants. The bioreactors were designed to be immersed in liquid media with plantlets with an adjustable immersion time. TIB and CIB improved germination rates up to 80.86% and 95.21%, respectively, however, CIB produced more hyperhydric plantlets than TIB. The height of plantlets in TIB was significantly higher than for those in CIB. Fresh weights of plantlets grown in CIB of were significantly lower than for those grown in TIB. The lowest chlorophyll concentration was found in in vitro plantlets from CIB. We examined abnormally developed leaves, stems, and apical zones of in vitro plantlets that were produced in CIB. Among the three types, SS showed the highest stomatal density and the shortest stomatal length in in vitro plantlets. After acclimatization, plants from CIB exhibited the lowest values in biomass, such as height, root collar diameter, leaf fresh weight, leaf length, leaf width, petiole length, petiole diameter, and leaf area. Photosynthesis and transpiration rates of ex vitro plants were not significantly different among the three culture types, but stomatal conductance was higher in TIB than in the SS and CIB. Therefore, the results suggest that TIB is the preferable bioreactor to improve in vitro plantlet regeneration of L. tulipifera. TIB-originated plants showed higher growth rate than SS and CIB after transferring to soil.

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Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

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Plant biotechnology can be used to conserve the germplasm of natural forests, and to increase the productivity and sustainability of plantations. Both goals imply working with mature trees, which are often recalcitrant to micropropagation. Conventional in vitro culture uses closed containers and gelled medium with sugar supplementation. Bioreactor culture uses liquid medium and usually incorporates aeration. The increased absorption of nutrients via the liquid medium together with the renewal of the air inside the bioreactors may improve the physiological state of the explants. In this review, we will explore the feasibility of using bioreactors to overcome the recalcitrance of many trees to micropropagation and/or to decrease the cost of large‐scale propagation. We will focus on the recent use of bioreactors during the multiplication, rooting (plant conversion in the case of somatic embryos), and acclimation stages of the micropropagation of axillary shoots and somatic embryos of forest trees (including some shrubs of commercial interest), in both temporary and continuous immersion systems. We will discuss the advantages and the main obstacles limiting the widespread implementation of bioreactor systems in woody plant culture, considering published scientific reports and contributions from the business sector.

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Micropropagation of the pistachio and its rootstocks by temporary immersion system

Cited by 58 publications

References 38 publications

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

Use of bioreactor systems in the propagation of forest trees

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