Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms

<i>In Vitro</i> Micropropagation of Himalayan Weeping Bamboo, <i>Drepanostachyum falcatum</i>

“…Similar results with BAP were also reported in different species of bamboo [7] [9]- [13]. Ramanayake and Yakandwala [14] used 2.0 mg/l BAP + 0.1 mg/l Kn in D. giganteus. Though in present study BAP when used alone gave better bud break as compared to BAP and Kn used.…”

Micropropagation of apple (Malus domesticaL. cultivar Douce de Djerba) throughin vitroculture of axillary buds

“…Similarly Hutchinson (1984) and Webster & Jones (1991) agree that the rate of browning of apple explants decreases considerably when they were collected in summer compared with those collected in winter or autumn. Ramanayake and Yakandawala (1997) confirmed that aseptic culture establishment and bud break depended upon degree of contaminations and physiological status of the collected explants during various seasons of the year.…”

In Vitro Propagation of Arundinaria callosa Munro- an Edible Bamboo from Nodal Explants of Mature Plants

“…This problem was, however, overcome by proper removal of brown leaf sheaths and quick transfer to fresh medium whenever browning appeared. Researches carried out on micropropagation of bamboo showed variation in species in response to levels of BAP for shoot multiplication as in the case of Dendrocalamus longispathus [9], Dendrocalamus giganteus [11] and Bambusa bambos [3]. The present investigation shows that 13.3 M BAP significantly induces shoot multiplication.…”

“…A serious problem preventing successful micropropagation of bamboo and other woody species is their reluctance to form root [13,14], and Arundinaria callosa is not an exception. The induction of rooting in many bamboo species require many additives such as coumarin, phloroglucinol and TDZ, particularly when the explant is taken from the nodal segments of adult plants [11,15]. Saxena and Bhojwani [9] achieved 73% rooting in Dendrocalamus longipathus in a medium supplemented with coumarin along with auxins (IAA and IBA).…”