Micropropagation of the endangered species Malus niedzwetzkyana for conservation biodiversity in Kazakhstan

Nurtaza, Aidana; Magzumova, Gulmira; Yessimseitova, Assel; Karimova, Venera; Shevtsov, Alexandr; Silayev, Dmitriy; Lutsay, Viktoriya; Ramankulov, Yerlan; Kakimzhanova, Almagul

doi:10.1007/s11627-021-10174-4

Cited by 8 publications

(5 citation statements)

References 53 publications

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“…2). The process and methods of in vitro propagation of Malus niedzwetzkyana were comprehensively described in our previous work, and we followed all conditions [20]. We adjusted salt and sucrose concentrations for rooting of M. niedzwetzkyana shoots.…”

Section: Resultsmentioning

confidence: 99%

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Conservation of Malus Niedzwetzkyana Dieck Ex Koehne Genotypes From Kazakhstan Resistant to Scab and Fire Blight Diseases

Nurtaza¹,

Pozharskiy

Dyussembekova³

et al. 2022

Preprint

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Southeast Kazakhstan is part of the center of origin and the natural habitat of wild apple trees. Wild apples and their hybrids are involved in domestic apple breeding as a source of resistance to biotic and abiotic stress. Malus niedzwetzkyana is a valuable endemic apple species included in the Red Book of Endangered Species of Kazakhstan. The present study evaluated 23 M. niedzwetzkyana genotypes from different populations for their resistance to scab and fire blight diseases using SCAR markers. We determined that all 23 genotypes contained a 430 bp resistance allele for marker OPB19, and 5 of them also had a 799 bp resistance allele identified by marker OPB18 for scab resistance. For fire blight, seven genotypes contained a 375 bp resistance allele and one genotype had a 397 bp resistance allele for the QTL FBF7 locus identified by markers AE10-375 and GE-8019, respectively. Eight genotypes with double resistance to scab and fire blight were selected for further in vitro amplification to develop a genetic pool for biodiversity preservation, apple breeding, and wild apple population revival, among other purposes. All in vitro regenerants were analyzed for the presence of apple chlorotic leaf spot virus, apple mosaic virus, apple stem grooving virus, apple stem pitting virus, and tomato ringspot virus. For each of the eight genotypes with double resistance, genetic profiles were developed based on 12 SSR markers. These genetic passports allow us to maintain the purity of the genetic pool and improve MAS in apple breeding.

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Section: Resultsmentioning

confidence: 99%

“…Identi cation of markers' location on the genome of Malus domestica, cultivar 'Golden Delicious' (assembly ASM211411v1) was performed by Primer BLAST (NCBI). propagation was applied [20]. The axillary buds from one-year-old shoots were used as the source material.…”

Section: Plant Materialsmentioning

confidence: 99%

Conservation of Malus Niedzwetzkyana Dieck Ex Koehne Genotypes From Kazakhstan Resistant to Scab and Fire Blight Diseases

Nurtaza¹,

Pozharskiy

Dyussembekova³

et al. 2022

Preprint

Self Cite

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“…Kakimzhanova et al [ 96 ] reported that simple sequence repeat analysis confirmed the reliability (genetic homogeneity) of their protocol for efficient large-scale micropropagation of Malus sieversii . In another species of Malus , Malus niedzwetzkyana , Nurtaza et al [ 97 ] using simple sequence repeat primers, detected no somaclonal variation between the mother plant and the micropropagated clones of this endangered species. Asadi-Aghbolaghi et al [ 98 ] analyzing the genetic stability of regenerated Stipagrostis pennata plants using SSR markers, detected no somaclonal variation in regenerated plants from somatic embryos of S. pennata .…”

Section: Discussionmentioning

confidence: 99%

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Ioannidis¹,

Tomprou²,

Mitsis³

et al. 2022

Plants

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Simple sequence repeat (SSR) markers were used to evaluate the genetic stability of the acclimatized micropropagated and regenerated plants of a high cannabidiol (H-CBD) and a high cannabigerol (H-CBG) variety of Cannabis sativa L. Shoot regeneration and proliferation were achieved by culturing calli in Murashige and Skoog basal medium (MS) supplemented with several concentrations of 6-benzyladenine (BA) or thidiazuron (TDZ). Calli derived mostly from stem explants, rather than leaves, cultured on MS supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) or combination of kinetin (KIN) with 1-Naphthaleneacetic acid (NAA) or 2,4-D. Rooting of the regenerated plantlets accomplished on half-strength MS medium supplemented with indole-3-butyric acid (IBA). Previous studies performed have developed an efficient in vitro micropropagation protocol for mass production. Both in vitro methodologies can be employed in genetic breeding via molecular techniques. The genetic stability of micropropagated and regenerated plants was accomplished using twelve SSR primer pairs that produced reproducible and clear bands, ranging from 90 to 330 bp in size, and resulted in amplification of one or two alleles, corresponding to homozygous or heterozygous individuals. The SSR amplification products were monomorphic across all the micropropagated and regenerated plants and comparable to mother plants. The monomorphic banding pattern confirmed the genetic homogeneity of the in vitro cultured acclimatized and mother plants as no somaclonal variation was detected in clones for these specific SSRs. Our results evidently suggest that the developed culture protocols for in vitro multiplication is appropriate and applicable for clonal mass propagation of the C. sativa varieties and demonstrate the reliability of this in vitro propagation system.

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“…All collected M. niedzwetzkyana genotypes were introduced into a culture medium according to the previously developed protocol for microclonal propagation in the Laboratory of Plant Biotechnology and Selection at the National Center of Biotechnology, Astana [26]. Axillary buds from one-year-old shoots were used as source material.…”

Section: In Vitro Cultivation Of M Niedzwetzkyana Genotypesmentioning

confidence: 99%

Wild Malus niedzwetzkyana Dieck ex Koehne as a Genetic Resource for Fire Blight Resistance

Kolchenko,

Nurtaza,

Pozharskiy

et al. 2023

Horticulturae

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Wild apples and their hybrids are valued as a source of genetic resistance to biotic and abiotic stress. Malus niedzwetzkyana is an endangered ornamental apple species endemic to Southeast Kazakhstan, the center of Malus domestication. To test the fire blight resistance of M. niedzwetzkyana, eight plant genotypes were inoculated with a local strain of Erwinia amylovora. The genotypes possess different genetic backgrounds, which was confirmed via SSR profiling. Four out of eight displayed moderate to severe symptoms of fire blight infection, while the three wild genotypes proved resistant. To search for the source of the resistance, the samples were tested for the presence of FBF7 QTL using SCAR markers, where seven genotypes tested positive for one of the markers (AE10-375) and one for the other (GE80-19). No correlation between resistance phenotype and FBF7 QTL was confirmed, indicating the source lies elsewhere. Developing detailed genetic and phenotypic profiles of wild apple species helps advance both the preservation efforts and marker-assisted selection in apple breeding.

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Micropropagation of the endangered species Malus niedzwetzkyana for conservation biodiversity in Kazakhstan

Cited by 8 publications

References 53 publications

Conservation of Malus Niedzwetzkyana Dieck Ex Koehne Genotypes From Kazakhstan Resistant to Scab and Fire Blight Diseases

Conservation of Malus Niedzwetzkyana Dieck Ex Koehne Genotypes From Kazakhstan Resistant to Scab and Fire Blight Diseases

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Wild Malus niedzwetzkyana Dieck ex Koehne as a Genetic Resource for Fire Blight Resistance

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