Micropropagation of Strawberry via Axillary Shoot Proliferation

Boxus, P.

doi:10.1385/1-59259-583-9:103

Cited by 25 publications

(28 citation statements)

References 2 publications

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“…Micropropagation of strawberry was reported about 30 years ago (Boxus, 1999). Mahmood et al (1994) reported in their study that the best shooting response was observed on the media supplemented with 0.5 mg/l BAP, which was same as present finding, while the number of leaves were 8 which was different from present result (i.e., 5 leaves).…”

Section: Discussioncontrasting

confidence: 49%

Micropropagation of Strawberry (Fragaria ananassa) through runner culture

Ashrafuzzaman

Faisal

Yadav

et al. 2013

Bangladesh J. Agric. Res

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In vitro propagation of strawberry was conducted at the Biotechnology Lab. of BARI, Joydebpur, Gazipur. For shoot induction, five BAP concentrations viz., 0.0 (Control), 0.5, 1.0, 1.5, and 2.0 mg/l and for root induction four IBA concentrations viz., 0.0 (Control), 0.5, 1.0, and 1.5 mg/l were used. The highest average number of shoots (7) and the highest average length (3.34 cm) of shoot was observed at the concentration of 0.5 mg/l BAP. The highest average number of leaves (5) was also observed at the same concentration. Among the five rooting concentrations, IBA @ 0.5 mg/l showed the best performance in all the parameters studied. The highest number (6) of roots/culture and the longest (3.05 cm) roots were also obtained from this concentration. Half strength MS media without IBA concentration did not show any response regarding root induction.

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Section: Discussioncontrasting

confidence: 49%

Micropropagation of Strawberry (Fragaria ananassa) through runner culture

Ashrafuzzaman

Faisal

Yadav

et al. 2013

Bangladesh J. Agric. Res

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“…The micropropagation of strawberry was achieved more than 30 years ago (Boxis, 1999) and now it seems to be a routine task in many commercial laboratories. In general, strawberry can be managed easily under in vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

Zobayer

Prodhan

Sikdar

et al. 2013

Int. J. Agril. Res. Innov. & Tech.

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Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained.

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“…Some concern has been expressed about the genetic stability of micropropagated plants (Dijkstra 1990, Graham 2005. Therefore, the technique declined in popularity (Zimmerman 1991) and was rejected or limited by law in some countries (Boxus 1999). Jemmalli et al (1992 and1994) found that adventitious (stipular) shoots occurred spontaneously, and were common in strawberry in vitro shoot cultures.…”

Section: Introductionmentioning

confidence: 99%

“…They also revealed the relationship between adventitious shoots arisen in vitro and plant hyperflowering. Boxus (1999) underlined the risks introduced by adventitious regeneration in mass propagation systems of strawberry. To avoid this, using a decreasing concentration of BA, shortening the subculture period, and limiting the number of subcultures is recommended (Jemmalli et al 1992, Graham 2005.…”

Section: Introductionmentioning

confidence: 99%

Development of in vitro shoot cultures of strawberry (Fragaria × ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

Litwińczuk

Okołotkiewicz

Matyaszek

2009

Folia Horticulturae

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K e y w o r d s: adventitious shoots, axillary shoots, propagation in vitro, runners ABSTRACT In this study, the influence of gibberellic acid (GA 3 ) on strawberry in vitro shoot culture growth and development was investigated. 'Senga Sengana' and 'Elsanta' clones were grown on the medium recommended by Boxus (1999), supplemented with BA (0.5 mg dm -3 ), IBA (0.1 mg dm -3 ), glucose (40.0 g dm -3 ) and GA 3 (0.1 -control, 1.0, 2.0, 5.0, 10.0 mg dm -3 ). In general, gibberellic acid improved axillary shoot elongation and reduced the growth of callus as well as the formation of roots and the development of adventitious shoots. GA 3 applied at a concentration of 1.0-2.0 mg dm -3 significantly increased the number of axillary shoots (mainly crown shoots), whereas under higher (5.0-10.0 mg dm -3 ) doses it stimulated the development of runners. It seems that a new method of strawberry micropropagation based on the multiplication of axillary crowns and runners,

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Micropropagation of Strawberry via Axillary Shoot Proliferation

Cited by 25 publications

References 2 publications

Micropropagation of Strawberry (Fragaria ananassa) through runner culture

Micropropagation of Strawberry (Fragaria ananassa) through runner culture

Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

Development of in vitro shoot cultures of strawberry (Fragaria × ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

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