Micropropagation of red sanders (Pterocarpus santalinusL.) using mature nodal explants

Abstract

In vitro clonal multiplication of Pterocarpus santalinus L. was achieved using mature nodal explants of a 10-year-old elite quality tree. Combinations of serial transfer technique and incorporation of antioxidants (250 mg/l l-ascorbic acid and 50 mg/l citric acid) into the culture medium helped to minimize medium browning and improve explant survival during shoot sprouting. About 70% of explants were sprouted on Murashige and Skoog (MS) liquid medium containing 4.4 mM 6-benzyladenine (BA). The explant harvest period also influenced the bud break and shoot sprouting in nodal explants. The combination of 4.4 mM BA and 2.2 mM thidiazuron (TDZ) was found to be the most suitable growth regulator for obtaining the highest percentage of nodal segment sprouting (74%-75%), the number of secondary shoots per primary shoot (two or three), the shoot length (5-6 cm), the number of new nodal segments generated per active explant (four or five), and the multiplication coefficient (3.5) within 6 weeks. Repeated subculturing of nodal explants obtained from shoot cultures enabled continuous production of healthy axillary shoots. At the end of the sixth passage, about 90% of nodal explants produced five or six healthy green shoots, each being about 6.6 cm long with six or seven nodes. Multiplication coefficient was also increased from the first subculture (5.4) to the sixth subculture (8.3). The best rooting response was achieved on solidified half-strength MS medium supplemented with 4.9 mM indole-3-butyric acid (IBA). About 70% of the micropropagated plantlets were established successfully in 20-cm pots containing a mixture of soil and farmyard manure (4 : 1 ratio) and formed new leaflets.

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

“…4c.). The serial transfer technique have been found to be beneficial in reducing phenolic leaching and improving explants survival in Teak (Tiwari et al 2002) and Pterocarpus santalinus (Prakash et al 2006). Antioxidant mixture containing PVP, ascorbic acid and citric acid has been previously used to minimize phenolic exudation and for healthy growth of cultures in Boswellia serrata (Purohit et al 1995).…”

In vitro plant regeneration system for Cassia siamea Lam., a leguminous tree of economic importance

“…Comparatively lesser number of roots were produced when IBA was replaced with other auxins, NAA and IAA. However, among all the three auxins tested the lower concentrations of auxins were more satisfactory than the higher concentrations because the higher concentration favored basal callusing before root induction, which resulted into poor vascular (Prakash et al 2006) and Clitoria ternatea (Shahzad et al 2007) where lower concentration of IBA was proved to be the best for rhizogenesis.…”

Review of Literature

“…This approach of increasing the yield of shoots at an enhanced pace was adopted earlier for other woody taxa (Kaveriappa et al 1997;Jain and Babbar 2000;Hiregoudar et al 2005;Prakash et al 2006;Tripathi and Kumar 2010;Shekhawat and Shekhawat 2011).…”