Micropropagation of Plumbago rosea Linn.

Abstract

Multiple shoot formation from the medicinal plant Plumbago rosea Linn. was induced on callus from stem segments on Murashige & Skoog media containing auxin and cytokinin. 2,4-D (2.5 mgl ~) and kinetin (1.5 mgl -t) added to the media gave best callus production, while BAP (2 mg 1 -j) plus NAA (1.0 mg 1 -~) induced shoot formation from that callus. Numerous shoots with roots could be produced by transferring shoots to media containing I BA (1.5 mg 1-~). Regenerated plantlets were transferred to pots and 60% survived.

In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh

“…Bhadra et al (2009) reported that leaf and nodal segments of two months old field grown seedlings of Plumbago indica L. were cultured on agar solidified MS supplemented with different concentrations and combinations of NAA, IAA, 2,4-D and picloram and BAP and Kn; the nodal segments produced either multiple shoot buds or callus of different nature depending on the combinations of plant growth regulators. A similar phenomenon was observed in Plumbago indica L. by other researchers (Gopalakrishnan et al, 2009;Kumar and Bhavanandan, 1988;Yogananth and Basu, 2009). …”

“…It is important, therefore to develop an efficient micropropagation technique for Plumbago indica L. for rapidly disseminate superior clones. Many important medicinal herbs throughout the world have been successfully propagated in vitro by organogenesis Chueh et al, 2001;Erdei et al, 1981;Hatano et al,1986;Hiraoka and Oyanagi, 1988;Huang et al, 2000;Matsumoto et al,1986;Nishioka, 1988;Shoyama et al, 1983;Tsay et al, 1989 explants (Bhadra et al, 2009;Gopalakrishnan et al, 2009;Kumar and Bhavanandan, 1988;Yogananth and Basu, 2009). Bhadra et al (2009) reported that leaf and nodal segments of two months old field grown seedlings of Plumbago indica L. were cultured on agar solidified MS supplemented with different concentrations and combinations of NAA, IAA, 2,4-D and picloram and BAP and Kn; the nodal segments produced either multiple shoot buds or callus of different nature depending on the combinations of plant growth regulators.…”

“…Bhadra et al (2009) reported that leaf and nodal segments of two months old field grown seedlings of Plumbago indica L. were cultured on agar solidified MS supplemented with different concentrations and combinations of NAA, IAA, 2,4-D and picloram and BAP and Kn; the nodal segments produced either multiple shoot buds or callus of different nature depending on the combinations of plant growth regulators. Gopalakrishnan et al (2009) and Kumar and Bhavanandan (1988) observed that multiple shoots were found by using different concentration of cytokinin with auxins through indirect organogenesis using leaf explants and nodal segments and Yogananth and Basu (2009) observed multiple shoots by using different culture media through direct organogenesis from nodal segments. The present study was therefore undertaken to develop a protocol for in vitro shoot proliferation and plant regeneration of rare medicinal herb of Bangladesh, Plumbago indica L. using shoot tip and nodal explants.…”

In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

“…Use of auxins singly or in combination for rooting was also reported by different authors (Bhadra et al, 2009;Gopalakrishnan et al, 2009;Hassan and Khatun, 2010;Kumar and Bhavanandan, 1988;Sivakumar and Krishnamurthy 2000;Yogananth and Basu, 2009). …”

Physiological, Biochemical and Modern Biotechnological Approach to Improvement of Rauwolfia serpentina

“…There are many reports on the regeneration of various medicinal plants via callus culture. Satheesh and Bhavanandan [12] have reported the regeneration of shoots from callus of Plumbagorosea using appropriate concentrations of auxins and cytokinins. Mantell and Hugo [13] have also reported a high frequency of shoot, root, and micro tuber production from Dioscoreaalatadepending on the culture medium used, the type of explant from which the calli originated, and the photoperiod.…”