Micropropagation of Pepper (Capsicum annuum L.) Through in vitro Direct Organogenesis Organogenesis

Otroshy, Mahmoud; Moradi, Kosar; Nekouei, Mojtaba Khayam; Struik, P.C.

doi:10.3923/ajbkr.2011.38.45

Cited by 15 publications

(8 citation statements)

References 13 publications

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“…An overview of all the results in the present study, noticed that maximum induction of multiple shoots took place on MS medium fortified with various concentrations of BAP with low concentration of IAA. Similar findings also observed by a few researchers [33][34][35][36]. This may be due to the synergistic effect of cytokinin (BAP) and auxin (IAA) has been demonstrated in many medicinal plants, for example Santolina canescens [37], Bupleurum fruticosum [38] and turmeric [39] who noticed that the low concentrations of an auxin in combination with a cytokinin influence the frequency of shoot induction and growth effectively on the other hand the high concentration of cytokinin induced the formation of stunted shoots has been observed [40,41].…”

Section: Resultssupporting

confidence: 92%

Development of a profused In vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell

Tata

2020

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Bacopa monnieri (L.) Pennell is an important medicinal plant used for the preparation of medhyarasayan (rasayana). Leaf explants of field grown young plants of B. monnieri was used to establish an efficient regeneration protocol with cytokinin (BAP) and auxin (IAA). The highest multiplication, i.e. (220 shoots/leaf, a cumulative of 2200 shoots from 10 explants) were noticed after 45 days of culture in MS medium supplemented with BAP(1.5mg/L) and IAA(0.5mg/L). The optimum concentration of growth regulator for shoot elongation and rooting was recorded in MS+GA3(0.25mg/L) and MS+IBA(1.5mg/L) respectively. The rooted plantlets were successfully established in green house conditions.

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Section: Resultssupporting

confidence: 92%

Development of a profused In vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell

Tata

2020

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“…Adventitious leaves developed mostly on the cotyledon petioles, where there were cuts in plant tissue or accidental injury of the initial explant while placing it in the medium (Figure 1). The organogenesis on the surface of the cuts is observed in pepper irrespective of the growth regulators applied (Dabauza and Peña, 2001;Gatz, 2002a;Joshi and Kothari, 2007;Otroshy et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…These 2 factors, therefore, showed no significant effect on the reaction of the initial explants. A simultaneous formation of adventitious leaves and callus tissue during pepper in vitro cultures was described by Phillips and Hubstenberger (1985), Rodeva et al (2006), Otroshy et al (2011), andSong et al (2010). Phillips and Hubstenberger (1985) as well as Qin et al (2005) proved that callus cells do not differentiate as a response to various organogenesisinducing factors, including the effects of BA with IAA, analyzed in this paper.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, certain genetic variations, characteristic of the biology of the plant, can occur during the reproduction of the sowing material and may cause some deviations from the cultivar standard. The possibility of vegetative in vitro mass propagation of pepper can overcome the difficulties listed above, providing an unlimited number of entirely homogeneous, healthy in vitro plantlets, independently of season (Song et al, 2010;Otroshy et al, 2011). According to Dabauza and Peña (2001), pepper micropropagation is a unique tool that is very convenient for maintaining the genetic homogeneity of the elite plant materials, as well as for preserving heterozygous genotypes and genetically transformed forms.…”

Section: Introductionmentioning

confidence: 99%

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In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

Orlinska¹,

Nowaczyk²

2015

Turk J Biol

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This study evaluates the effectiveness of in vitro regeneration of 4 genotypes of pepper (Capsicum spp.) that differ in origin and functional properties: California Wonder, (ATZ × Sono)F 1 , Jalapeno, and SF-9. In order to investigate the effect of the initial explant type, photoperiod, and presence of 2.0 mg L -1 of glycine in the medium, organogenesis was induced on the MS medium with 2.0 g L -1 2-(N-morpholine) ethanesulfonic acid, 1.7 mg L -1 AgNO 3 , 0.4 mg L -1 indole-3-acetic acid (IAA), and 6.0 mg L -1 6-benzyladenine. The effectiveness of organogenesis in the 3 genotypes representing C. annuum L. was similar and considerably lower for line SF9 derived from an interspecific hybrid (C. frutescens L. × C. annuum L.). The highest number of adventitious leaves was recorded in the decapitated seedlings exposed to continuous light. The lack of glycine in the medium favored direct organogenesis. A simultaneous formation of adventitious leaves with a stable number of chromosomes and mixoploid callus tissue was determined by flow cytometry. The successive stage of foliaged shoots and roots formation was most effective in the MS medium with 1.1 mg L -1 IAA. The use of these optimal conditions resulted in developing 42%-56% of properly formed diploid plantlets, successfully acclimatized in 86%-97%.

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“…This highly supports the fact that its extinguishing germplasm should be conserved urgently Commensurate with this, intervention of biotechnology or to be more precise, plant tissue culture for accelerating clonal multiplication of desired clones and strains of medicinal plants through micropropagation and their conservation through establishing Tissue Banks or Gene Banks are warranted in the right earnest. Even though there are several reports of In vitro regeneration on different domesticated species of Capsicum [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15], there is no such report on indirect organogenesis of C. chinense cv. Lota Bhot however.…”

Section: Introductionmentioning

confidence: 99%

MICROPROPAGATION OF Capsicum chinense Jacq. cv. LOTA BHOT VIA INDIRECT ORGANOGENESIS

Bora¹,

HK²,

PJ³

2014

Int J of Agri Scie

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Capsicum chinense Jacq. is well known for its pungency factor, "capsaicin". An attempt has been made to maintain the desired pungency of Capsicum chinense Jacq. cv. Lota Bhot through in vitro propagation via callusogenesis from leaf explants under the influence of different plant growth hormones and an additional component, Silver nitrate (AgNO3). The combination of Kinetin (45µML -1 ) along with 2,4-Dichlorophenoxy Acetic Acid (2,4-D) and AgNO3 at 3.5µML -1 and 35µML -1 respectively was found optimum for callus induction as well as multiple shoot induction whereas maximum shoot length was recorded at Kin (40µML -1 ), 2,4-D (3.0 µML -1 ) and AgNO3 (30µML -1 ). In vitro flower induction and fruit development was most observed under the influence of Gibberellic Acid (GA3) at 30µML -1 . Murashige and Skoog (MS) medium fortified with Benzyl Amino Purine (BAP) 1 and 2,4-D at 4.0µML -1 and 5.5 µML -1 correspondingly was standardized for maximum induction of roots in vitro. Healthy in vitro regenerated plantlets were acclimatized in the potting substrate containing half strength MS medium enriched with soil, sand, vermicompost and vermiculite in all equal proportion and 78.56% of total acclimatized plants were successfully transferred to the main field.

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Micropropagation of Pepper (Capsicum annuum L.) Through in vitro Direct Organogenesis Organogenesis

Cited by 15 publications

References 13 publications

Development of a profused In vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell

Development of a profused In vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell

In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

MICROPROPAGATION OF Capsicum chinense Jacq. cv. LOTA BHOT VIA INDIRECT ORGANOGENESIS

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