Micropropagation of Orchid Dendrobium barbatum Lindl. (Orchidaceae) from Fleshy Stem Segments

Abstract

An efficient protocol for micropropagation of orchid Dendrobium barbatum Lindl. (Orchidaceae) using the fleshy stem segments was developed. Murashige Skoog (MS) basal medium supplemented with 1.0 mg L of Naphthalene Acetic Acid (NAA) + 2 .0mg L of Benzyl Amino Purine (BAP) for multiple shoot induction. The multiplied shoots were then transferred to half-strength MS medium supplemented with 1 mg L of Indole-3 acetic acid (IAA) or Indole-3 butyric acid (IBA) or NAA. Best response for shoot multiplication was achieved when the medium was supplemented with 1 mg L-1 NAA and 2mg L-1 BAP. While that for rooting with 1 mg l IBA. The plantlets were hardened off and survived plants were released to the shade house then to their epiphytic environment.