Micropropagation of Operculina turpethum (L.) Silva Manso through Nodal Segment, Apical Bud and Cotyledon

Abstract

Aim: To develop in vitro propagation protocol of a rare, vulnerable and endangered important medicinal plant Operculina turpethum (L.) Silva Manso (Trivrit) through organogenesis. Materials and methods: Seeds collected from Institute's garden were pretreated and inoculated on Murashige and Skoog (MS)medium. Cotyledon, axillary bud and nodal segments of in vitro grown plants were used as explants. Explants were cultured on half MS, MS, whites plain medium and MS supplemented with different concentrations and combinations of plant growth regulators viz., BAP, AS, Kn, NAA, IAA, IBA. Cultures were incubated at 22 o C + 2 o C and 8 hours photoperiod with light intensity of 3000 lux. Observations were recorded at an interval of 15 to 25 days. Result:The average maximum number of shoots 19.72 + 0.240 achieved on MS supplemented with BAP (3 mg/L). 100% root induction was obtained on half MS, MS, Whites medium alone and the combination of MS with 1 to 4 mg/L concentrations of NAA, IBA, IAA. In vitro developed plantlets were transferred in the pots; which were easily acclimatized and established in the soil. Conclusion:The developed micropropagation protocol is beneficial for the rapid proliferation of shoots and root. The protocol would be helpful for mass multiplication as well as to conserve the rare and endangered plant of Trivrit.