2018
DOI: 10.5005/jp-journals-10059-0038
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Micropropagation of Operculina turpethum (L.) Silva Manso through Nodal Segment, Apical Bud and Cotyledon

Abstract: Aim: To develop in vitro propagation protocol of a rare, vulnerable and endangered important medicinal plant Operculina turpethum (L.) Silva Manso (Trivrit) through organogenesis. Materials and methods: Seeds collected from Institute's garden were pretreated and inoculated on Murashige and Skoog (MS)medium. Cotyledon, axillary bud and nodal segments of in vitro grown plants were used as explants. Explants were cultured on half MS, MS, whites plain medium and MS supplemented with different concentrations and co… Show more

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