Micropropagation of mature Crataegus aronia L., a medicinal and ornamental plant with rootstock potential for pome fruit

Abstract

The effects of culture media and cytokinin types on micropropagation of mature Crataegus aronia L. were investigated. Using single-axillary bud explants, the growth of cultures on MS, WPM, DKW and NRM containing 4.44 lM benzyladenine (BA) plus 0.05 lM indole-3-butyric acid (IBA), and on NRM containing thidiazuron, metaTopolin (mT) or BA at 1.25, 2.5, 5.0 or 7.5 lM plus 0.05 lM IBA were compared. The culture medium had significant effects on shoot number and length. In comparison with MS, DKW and WPM, shoot production was greater on NRM (5.7 shoots per explant). Shoot production on MS, DKW and WPM (4.2, 4.2 and 4.1, respectively) were statistically similar to each other. Thidiazuron was detrimental to shoot formation and caused formation of rosette shoots and/or large callus to form on explants. In the presence of mT, only some of the explants developed into shoots. Benzyladenine was the only cytokinin that promoted both shoot proliferation and shoot elongation. Higher shoot numbers were obtained at 5.0 and 7.5 lM BA compared to lower concentrations of BA. Over 80% of microshoots rooted and rooted shoots were successfully acclimatized to ex vitro conditions.

Microclonal Propagation of Crataegus Monogyna Jacq. In Vitro

“…Most of them prefer Murashige and Skoog Medium (Dai et al, 2007;Maharic et al, 2009;Iapichino and Airo, 2009). Some of them use WPM, DKW and NRM mediums (Nas et al, 2012). Crataegus Azarolus L. is propagated on the modified LP nutrient medium (Caboni et al, 2010).…”

Effects of charcoal and physical state of medium on micropropagation of black mulbery (Morus nigra L.)

“…18 o C -max. 27 o C) and relative humidity conditions (Nas et al, 2012). For each treatment and genotype there were three replicate jars with 5 shoots per jar, and all experiments were repeated three times.…”

Shortcut to long-distance developing of a tissue culture medium: micropropagation of mature almond cultivars as a case study

“…The rooting experiment was conducted 4 times, and each time at least 2 replications (Magenta GA7 vessels containing 4-9 shoots) of each genotype were used. The rooting and acclimatization procedures reported by Nas et al (2012) were applied.…”