Micropropagation of Laburnum anagyroides Medic. through axillary shoot regeneration

Timofeeva, S. N.; Elkonin, L. A.; Tyrnov, V. S.

doi:10.1007/s11627-014-9618-0

Cited by 19 publications

(7 citation statements)

References 27 publications

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“…Addition of 2.0 mg l −1 TDZ resulted in hyperhydric shoots. A similar result has been reported in other woody species such as C. wilsonii (Sivanesan et al, 2011), Laburnum anagyroides (Timofeeva et al, 2014) and Ulmus glabra (Mirabbasi and Hosseinpour, 2014).…”

Section: Axillary Shoot Multiplicationsupporting

confidence: 87%

Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (Michx.) Elliott

Sivanesan

Saini

Kim

2016

Industrial Crops and Products

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Section: Axillary Shoot Multiplicationsupporting

confidence: 87%

Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (Michx.) Elliott

Sivanesan

Saini

Kim

2016

Industrial Crops and Products

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“…2). It is well known that higher concentrations of cytokinin promote shoot hyperhydricity in many plant species, for example Salvia przewalskii, Aloe polyphylla, Laburnum anagyroides, and Scutellaria alpina (Skała et al 2007;van Staden 2008, 2011;Timofeeva et al 2014;Grzegorczyk-Karolak et al 2015). However, as it was observed in our study, the frequency of hyperhydric shoots in T. scorodonia culture did not evidently depend on cytokinin concentrations, but occurred more frequently when nodal segments were used as the explants.…”

Section: Micropropagation Of T Scorodoniasupporting

confidence: 47%

In vitro propagation, DNA content and essential oil composition of Teucrium scorodonia L. ssp. scorodonia

Makowczyńska

Śliwińska

Kalemba

et al. 2016

Plant Cell Tiss Organ Cult

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Teucrium scorodonia ssp. scorodonia is a valuable medicinal plant. It is rare in Poland, therefore availability of raw material is limited and an effective method of producing in vitro-derived plants for pharmaceutical reasons would be desirable. However, the protocol for micropropagation of this species has not been developed yet. Here, its regeneration ability on media supplemented with different growth regulators was evaluated using shoot tip and nodal segment explants.

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“…The acclimatization of in vitro regenerated plantlets was a difficult step of the micropropagation protocol establishment of their susceptibility to fungal diseases 53 . In the present study, the healthy rooted plantlets (Fig.…”

Section: Resultsmentioning

confidence: 99%

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Sultana

Das

Chandra

et al. 2022

Sci Rep

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Thunbergia coccinea Wall. ex D. Don being a rare, ornamental and medicinal plant of India, is needed to propagate for conserving the germplasm and analyzing its phytochemical compounds in the future. A reliable protocol for direct in vitro propagation using nodal shoot meristem of T. coccinea as explant was standardized. The highest number of shoots per explant (22.17 ± 0.54) with maximum shoot length (2.36 ± 0.28) in cm was obtained in Murashige and Skoog (MS) medium supplemented with 9.70 µM of 6-furfurylaminopurine (Kinetin) and 0.053 µM of α-naphthaleneacetic acid (NAA) combination, among all the different plant growth regulators (PGR’s) and concentrations tested. The aforesaid PGR’s combination was optimum for axillary shoot bud induction and multiplication in T. coccinea. The best rooting was observed on the half-strength MS medium fortified with 2.68 µM NAA with the highest number of roots per shoot (3.75 ± 0.12) and maximum length (5.22 ± 0.32) in cm. All the in vitro raised plantlets were acclimatized in sterile sand and soil mixture (1:1) with a survival rate of 70% on earthen pots under greenhouse conditions. PCR-based RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat) molecular markers were employed to determine the genetic homogeneity amongst the plantlets. Twelve (12) RAPD and nine (9) ISSR primers developed a total of 104 and 91 scorable bands, respectively. The band profiles of micropropagated plantlets were monomorphic to the mother, donor in vivo plant, and similarity values varied from 0.9542–1.000. The dendrogram generated through UPGMA (unweighted pair group method with arithmetic mean) showed 99% similarities amongst all tested plants confirming the genetic uniformity of in vitro raised plants.

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Micropropagation of Laburnum anagyroides Medic. through axillary shoot regeneration

Cited by 19 publications

References 27 publications

Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (Michx.) Elliott

Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (Michx.) Elliott

In vitro propagation, DNA content and essential oil composition of Teucrium scorodonia L. ssp. scorodonia

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

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