Micropropagation of Japanese persimmon (Diospyros kakiThun.) cv. ‘Hiratanenashi’

Abstract

Axillary buds of Japanese persimmon (Diospyros kaki Thun. cv. 'Hiratanenashi') were decontaminated, their budscales removed aseptically and placed in modified Murashige and Skoog (MS) medium containing 4.5~zeatin. Over 60% of the buds initiated shoots in 6 weeks. A multiplication rate of over lO-fold could be achieved in 6-8 weeks by two transfers (shoot proliferation and shoot elongation) in the same medium. Attempts to initiate shoots from meristem tips were unsuccessful. Using shoots that are > 25 mm long, c. 85% rooting was accomplished over a 6-week period in diluted (nitrogen content, 1/4 strength; other nutrients, 1/2 strength) MS medium containing 2% activated charcoal and 4.9~indole-3-butyric acid (IBA) with a liquid overlay of the undiluted medium containing 4.5~trans-zeatin (t-Z). Techniques are also described for the regeneration of whole plants from leaf segments (from in vitro propagated plants) using a combination of t-Z (10~and anyone of three auxins tested-indole-3-acetic acid, IBA or (Xnaphthalene acetic acid (0.001-0.01 JlM). The presence of auxins was not essential but slightly accelerated the organogenic callus formation and organogenesis. Rooted plantlets were planted into a mixture of peat, perlite, and CaC0 3 and acclimatised in a fog tent for 4-8 weeks.

Callus induction and Cytology of Vulnerable Tree Species Diospyros candolleana Wight

“…Tao and Sugiura, (1992) reported that, hard globular calli proliferated from leaf primordial of D. kaki. In the earlier stage callus showing yellowish white colour later it became brown to black, similarly leaf explants also turns to brown or black colour gradually after inoculation because of the reason of exudation of polyphenol like substances was reported by Sugiura, 2008;Choi et al, 2001;Sarathchandra and Burch, 1991.…”