Micropropagation of Helianthemum lippii L.var sessifolium

Salih, Aya Mohui Aldeen; Omran, Zainab Sabeeh; Al-Ani, Nabeel K.

doi:10.5296/jbls.v10i1.13754

Cited by 1 publication

(3 citation statements)

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“…The shoot, node and apical parts of the plant were successfully used as an initial explant in tissue culture of Helianthemum sp. genera, as well as in many plants [24,25,26,27,28,29]. In the present study, depending on the development of explants, the media in which they were planted and the PGR combinations they contain, callus, shoot and root formations were observed.…”

Section: Resultsmentioning

confidence: 53%

“…Serrano-Martinez and coauthors in H. marminorense, Hamza and coauthors and Salih and coauthors in H. lippii L. var. sessiliforium, [26,27,29] used only MS and DKW basal media. In our study, an optimization method was applied for in vitro propagation of H. germanicopolitanum using three different basal media together with PGR combinations.…”

Section: Initial Shoot Lengthmentioning

confidence: 99%

“…Serrano-Martinez and coauthors (2012) reported that 0.5 mg/L 2iP containing the WPM medium provided the best shoot number in H. marminorense [26], Hamza and coauthors (2013) found maximum shoot number and shoot development for H. lippii L. var. sessiliforium in the MS medium containing 0.5 and 1.0 mg/L BA [27], and Salih and coauthors (2019) studied the same species reporting that the highest shoot development occurred in different doses (0.5-1.0-1.5-2.0 mg/L) of BA, which was also cultured in the MS medium [29].…”

Section: Initial Shoot Numbermentioning

confidence: 99%

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In vitro Propagation to Conserve the Local Endemic and Endangered Medicinal Plant Helianthemum germanicopolitanum Bornm.

Kapdan

Sezgin

2021

Braz. arch. biol. technol.

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In this study, in vitro propagation and acclimatization of Helianthemum germanicopolitanum Bornm. plant, a local endemic in Çankırı Province (Turkey) with arid and semi-arid lands, and an endangered species taking part among medicinal and aromatic plants were accomplished, which is under-researched. In this study, three basal media [a) Murashige and Skoog b) Gamborg's B5, and c) Nitsch & Nitsch], two gelling agents (agar 7 g/L, and gelrite 2.1 g/L), eight cytokinins and eight auxin doses of plant growth regulators [a) 6-benzyladenin, b) Kinetin-(0, 0.5, 1, and 2 mg/L), c) Indole-3-butyric acid, d) α-napthaleneacetic acid-(0, 0.25, 0.5, and 1 mg/L)] prepared in 64 different combinations with 30 g/L sucrose was added to the basal media and adjusted to pH 5.7 for in vitro propagation of H. germanicopolitanum. During in vitro propagation of the plant, external and internal infections were frequently encountered and this was solved by the developed protocol. The best shoot growth (1.141 cm) and shoot length (0.572 cm) were obtained in the Gamborg's B5 medium in combination with Kinetin (0.5 mg/L)+Indole-3-butyric acid (0.5 mg/L)+gelrite. The maximum number of shoots (19.50) and the best multiplication rate (94%) were obtained in the media containing benzyladenin (1 mg/L)+Indole-3-butyric acid (0.5 mg/L) plant growth regulator in Murashige and Skoog medium solidified with agar. At the rooting stage, the maximum number of roots (30) was reached in the Murashige and Skoog medium containing gelrite and the best rooting rate (92%) with agar. A hundred plants representing the best shoot and root growth were taken to acclimatization stage, and 32 of these plants adapted to external conditions.

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