Micropropagation of Hagenia abyssinica: a multipurpose tree

Abstract

An in vitro propagation protocol has been developed for Hagenia abyssinica using original material from both juvenile and mature trees. Juvenile explants were obtained from seedlings, as well as shoots and meristems from 5 to 7-month-old greenhouse grown plants. Shoots collected from stem bases of five genotypes were used to establish cultures from mature trees. Explants of seedling origin were used to optimize the multiplication medium and growth regulators concentration. The best result was obtained from shoots subcultured on either MS or WPM medium supplemented with 4.4 lM BAP and 0.49 lM IBA. The initiated shoots from all the different explants were multiplied on these media. Rooting of shoots was achieved using MS medium containing macronutrients at one-third strength supplemented with 4.9 lM IBA. The shoots were kept in the dark for 4 days and transferred to medium of the same composition but containing 0.3% activated charcoal without growth regulators. Up to 100% rooting was achieved depending on genotype. Shoots multiplied on MS medium rooted better than those multiplied on WPM. Plantlets were transferred to pots containing a mixture of soil and perlite in a 2:1 ratio, respectively, and were maintained in the greenhouse. Increased irradiance reduced stem and leaf lengths and increased branch number of micropropagated plants.

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

“…1e). Similar results have been reported in research on rooting of other species, for example Artemisia judaica (Liu et al, 2003) and Hagenia abyssinica (Feyissa et al, 2005). The presence of callus and inhibition of root growth may be associated with low survival of plants (Tab.…”

“…3). A similar two-step rooting procedure has been used successfully for different species (Martin, 2003;Feyissa et al, 2005;Siddique and Anis, 2007;Husain et al, 2008;Conde et al, 2008). IBA at high concentration is known to suppress root elongation (Korach et al, 2002).…”

Establishment, multiplication, rooting and acclimatization of Cabralea canjerana (Vell.) Martius

“…Similar results were observed in explants of seedling from Hagenia abyssinica (Bruce J.F. Gmel), which presented either a similar in vitro growth, when cultivated in MS or WPM media (Feyissa et al, 2005). In contrast, plantlets of Punica granatum (L.) cultivated in WPM medium were apparently more vigorous and with longer shoots when compared with MS culture medium, which is an important feature in micropropagation (Valizadeh et al, 2013).…”

An efficient in vitro process for recurrent production of cloned plants of Vitex negundo L

“…In contrast, deleterious effect of combination of NAA with BA was also reported in Artemisia judaica (Liu et al 2003) and Garcinia indica (Malik et al 2005). However, in Hagenia abyssinica (Feyissa et al 2005), the best combination was observed on a medium containing BA and IBA, whereas IAA and BA combination was the optimum in Chonemorpha grandiflora (Nishitha et al 2006). Almost similar response was observed with BA and IBA or NAA combination in case of Pogostemon heyneanus (Hembrom et al 2006).…”