Micropropagation of flowering dogwood (Cornus florida) from seedlings

Kaveriappa, K. M.; Phillips, Lorelle; Trigiano, Robert N.

doi:10.1007/bf01092771

Cited by 25 publications

(24 citation statements)

References 7 publications

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“…After using TDZ, either alone or in combination with BAP or NAA, shoots often exhibited fasciated growth patterns. Similar situation was observed in C. florida when TDZ inhibited shoot elongation and produced numerous hyperhydric shoots (Kaveriappa et al 1997).…”

Section: Discussionsupporting

confidence: 54%

“…For this reason, modifications in the composition of basal WPM were undertaken which greatly improved the health status of shoots and supported a long-term proliferation growth of multiple shoot cultures. In tissue culture studies with C. florida, WPM proved superior to Murashige and Skoog as well as Schenk and Hildebrandt basal media (Kaveriappa et al 1997). Similarly, WPM macronutrients were superior to Anderson's, N6, Murashige and Skoog, Nitsch and Nitsch, and Quoirin and Lepoivre's macronutrients (Declerck and Korban 1994).…”

Section: Discussionmentioning

confidence: 93%

“…However, as C. florida does not prefer calcareous soils there was no need to modify concentrations of basal calcium salts in the above experiments. With regard to shoot multiplication rates in C. florida, Kaveriappa et al (1997) reported the highest mean number of shoots per explant between 5.5 and 6.0 which was achieved with 4.4 lM (i.e. 1.0 mg l -1 ) BAP.…”

Section: Discussionmentioning

confidence: 94%

“…Promising results from tissue culture studies were achieved mainly in flowering dogwood (C. florida). Regeneration of plantlets was based either on axillary shoot proliferation and adventitious rooting of elongated microshoots (Kaveriappa et al 1997) or on somatic embryogenesis and germination of somatic embryos into somatic seedlings (Trigiano et al 1989). Main drawbacks of the above studies were pertinent to the juvenile stage of mother explants because those experiments employed several week old seedlings or zygotic embryos.…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of mature Cornus mas ‘Macrocarpa’

Ďurkovič

2008

Trees

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Sprouting axillary buds sampled from a mature 27-year-old shrub of Cornus mas 'Macrocarpa' were used as starting material for in vitro culture establishment. Multiple shoot cultures, grown on basal woody plant medium with the pH adjusted to 5.6-5.7 and supplemented with 6-benzylaminopurine in combination with 1-naphthaleneacetic acid, were capable of continuous axillary and adventitious shoot proliferation up to 1 year. Later on, growth ceased, shoot tip necrosis appeared and shoot cultures died. Transfer of living shoots onto modified woody plant medium with the pH adjusted to 6.8-7.0 led to vigorous growth of multiple shoot cultures without any loss of multiplication rates or decreased vitality for several years. The use of 6-benzylaminopurine in combination with 1-naphthaleneacetic acid proved superior to the application of thidiazuron which induced a frequent formation of short and fasciated shoots. 1-naphthaleneacetic acid promoted in vitro adventitious rooting frequency up to 73.3%, whereas indole-3-butyric acid was not effective. Ex vitro acclimatized plants did not show any visually detectable morphological variation.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Micropropagation of mature Cornus mas ‘Macrocarpa’

Ďurkovič

2008

Trees

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“…This approach of increasing the yield of shoots at an enhanced pace was adopted earlier for other woody taxa (Kaveriappa et al 1997;Jain and Babbar 2000;Hiregoudar et al 2005;Prakash et al 2006;Tripathi and Kumar 2010;Shekhawat and Shekhawat 2011).…”

Section: Subculture Passagesmentioning

confidence: 95%

Review of Literature

Varshney¹,

Anis²

2014

Trees: Propagation and Conservation

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Gottlieb Haberlandt (Math Naturwiss 111:69-92), a German plant physiologist, for the first time initiated the work on tissue culture. His work arose as a research tool and attempts were made to culture the isolated, fully differentiated cells in nutrient medium in vitro as early as 1898. The theoretical basis of tissue culture lies in the cell theory given by Schleiden and Schwann (1838-1839). Practically, this technique stands on the concept of "totipotency," i.e., each cell has the ability to regenerate into a new plant. The field finds a wide range of applications starting from mass clonal propagation to plant improvement, molecular biology, bio-processing as well as a basic research tool. It has advanced the production in forestry and agriculture to many folds. There are a number of reviews published on tissue culture of woody and tree species which provide the wide-ranging micropropagation reports of various plant species. In this way, this chapter highlights the current review in tree tissue culture in vitro and micropropagation of other valuable plants, their significance, and the wide scope existing for investigations on mass multiplication and conservation of these plants.

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Micropropagation ofSchizandra chinensis BAILLON using glucose from cotyledonary nodes

et al. 2004

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A protocol for the micropropagation of Schizandra chinensis has been developed using regenerated shoots from axillary bud explants. In preparing to do so, we found that seed type (i.e., mature vs. pre-mature) significantly influenced the rate of germination. The Woody Plant (WP) medium proved to be superior to the Murashige and Skoog (MS) medium for germination purposes. Multiple shoots were induced from cotyledonary nodes of axenic seedlings on WP media containing 6-benzylaminopurine (BA) alone or in combination with 1-napthaleneacetic acid (NAA). High frequencies of shoot proliferation and the greatest number of shoots per explant (11.6) were observed with the use of 1.0 mg L 1 BA. We also established a culture method for proliferating shoots by repeatedly subculturing the original cotyledonary nodes on a shoot multiplication medium each time newly formed shoots were harvested. To induce root formation, glucose was supplied as a carbon-source substitution for sucrose. The best rooting rate was obtained from a WP medium supplemented with 3% glucose and 0.5 mg L -1 NAA. Following transplantation in the field, 82% of the plantlets survived.

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Micropropagation of flowering dogwood (Cornus florida) from seedlings

Cited by 25 publications

References 7 publications

Micropropagation of mature Cornus mas ‘Macrocarpa’

Micropropagation of mature Cornus mas ‘Macrocarpa’

Review of Literature

Micropropagation ofSchizandra chinensis BAILLON using glucose from cotyledonary nodes

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