Micropropagation of Flower Bulbs: Lily and Narcissus

Langens-Gerrits, Merel; Klerk, G.J.M. de

doi:10.1385/1-59259-583-9:141

Cited by 15 publications

(18 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bulblets were regenerated on scale explants, essentially as described previously (Langens‐Gerrits and De Klerk 1998). Various lily hybrids and species were used.…”

Section: Methodsmentioning

confidence: 99%

“…Unless stated otherwise, explants were cultured in the dark for 12 weeks at 25°C and subsequently for 4 weeks at 15°C. Then, regenerated bulblets were excised from the explant and kept at 5°C for 10 weeks on moist filter paper in Petri dishes to break dormancy (Langens‐Gerrits and De Klerk 1998). The bulblets were weighed individually before they were planted in soil and grown at 17°C under white light (Philips) at 70 µmol m −2 s −1 for 16 h per day.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Phase change in lily bulblets regenerated in vitro

Langens-Gerrits¹,

Klerk²,

Croes

2003

Physiologia Plantarum

View full text Add to dashboard Cite

During the development of the lily (Lilium), three phases can be distinguished: the juvenile, the vegetative adult and the flowering phase. Juvenile bulblets sprout with one or a few leaves whereas vegetative adult bulblets sprout with a stem with elongated internodes. The transition to the vegetative adult phase was studied in lily (Lilium × cv. Star Gazer) bulblets regenerating on bulb scale segments in vitro. The phase change was marked by the development of a tunica‐corpus structure in the apical meristem which leads to the formation of an actively growing stem primordium. This structure is absent in juvenile bulblets. Juvenile bulblets first developed competence for phase change during a culture period of at least 6 weeks at 25°C. Subsequent induction of the phase change occurred during a period of 2 weeks at lower temperature (15°C). A major factor influencing phase transition was bulblet weight. Small bulblets never formed a stem whereas large bulblets always formed a stem under inducing conditions. Large bulblets more often formed a stem than small ones but the relation between bulb growth and phase transition was not absolute. A high sucrose concentration, a large explant and a prolonged period for competence development stimulated bulb growth but also phase transition independently of growth. Lowering the concentration of MS‐minerals reduced bulb growth but did not affect phase transition. Under these conditions, phase change was correlated with a low phosphorus content.

show abstract

“…Bulblets were regenerated on scale explants, essentially as described previously (Langens‐Gerrits and De Klerk 1998). Various lily hybrids and species were used.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Phase change in lily bulblets regenerated in vitro

Langens-Gerrits¹,

Klerk²,

Croes

2003

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…Clean and healthy-looking scales were rinsed in 70% ethanol, sterilized in 1% (w/v) NaOCl for 30 min and rinsed three times in sterile de-ionized water. Bulblets were regenerated from scale explants essentially as described by Langens-Gerrits and De Klerk (1998). Large (3 ¿ 15 mm) and small (3 ¿ 5 mm) explants, cut as shown in Fig.…”

Section: Regeneration Of Bulbletsmentioning

confidence: 99%

Contribution of explant carbohydrate reserves and sucrose in the medium to bulb growth of lily regenerated on scale segments in vitro

Langens-Gerrits¹,

Kuijpers²,

Klerk³

et al. 2003

Physiologia Plantarum

View full text Add to dashboard Cite

Bulb size is an important factor determining phase change in Lilium: phase change only occurs in bulblets over a certain threshold weight. After phase change has occurred, bulblets sprout with a stem with many leaves. Juvenile bulblets sprout with only a few leaves. The factors contributing to bulb size were studied during in vitro regeneration of bulblets on scale segments. The larger the explants, the larger the regenerated bulblets. Explant size influenced bulb growth during the complete culture period. Bulb growth was stimulated by a high sucrose concentration. The contribution of the medium and the explant reserves to bulb growth were studied in large and small explants using labelled sucrose. Sucrose was mainly taken up through the cut surfaces. In freshly cut explants, the rate of uptake was correlated with the size of the contact area, but at later stages, when regenerating organs were present, the difference in uptake rate of small and large explants almost disappeared. Small explants had a larger sink activity than large ones. Explants with regenerating organs took up more sucrose than freshly cut explants. Sucrose uptake and bulb growth were rather constant in the later phases and doubled when the sucrose concentration was doubled. Partitioning of label from the sucrose over the various organs, was also rather constant in time: approximately 25% was accumulated in the bulblets, 35–45% in the explant and 30–35% was converted to CO2. About half of the label in the explant was recovered at the proximal side where regeneration takes place. Sucrose, once incorporated in the storage pools in the explant, either remained in the explant or was converted to CO2. Redistribution to the growing bulblets hardly occurred. The percentage of bulb growth that could be attributed to uptake of medium components was constant over the regeneration period: 45–50% for large and 65–75% for small explants.

show abstract

“…In March 2016; both conventional and in vitro grown bulblets were kept at 5°C for 08 weeks on moist filter paper in petri dishes to break dormancy (Langens-Gerrits and De Klerk, 1999) and then later in April 2016 planted in pots (9 cm in diameter and 15 cm in depth) containing fertilized soil with 380, 290, and 340 mg L −1 of N:P:K (Tanekura No. 42; Sumirin Agricultural Industry Co., Ltd., Japan).…”

Section: Bulblet Grown In Soilmentioning

confidence: 99%

“…Various studies were done on techniques, to analyze the complex multivariate data sets to break down the complexity in a simpler means to a better understanding of the complex effects of the variables involved during the in vitro bulb production on the in vitro culture and the in vivo performance to an improvement of the process. In general bulb production under conventional process is done by 'scaling' (scaling-excised scales are placed in moistened vermiculite to produce small bulblets generally, 1-4 per scale) (Langens-Gerrits and De Klerk, 1999). On the other hand, the scaling process is very slow and requires a longer period of time to bulblet growth (size) and ontogenic development.…”

Section: Introductionmentioning

confidence: 99%

Factors Affecting Bulblet Growth of Lilium sp. – A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway

Islam¹,

Shimasaki²

2019

Preprint

View full text Add to dashboard Cite

Lily–belong to the genus Lilium is one of the top cut flowers worldwide. Production and propagation of bulblets in vitro is an important approach for high-volume production, but not proved satisfactory. Hence, the aim of this study was to describe and compare the performances of morphological characteristics of the lily bulb in vivo produced by in vitro and conventional culture method and compare the production timelines in vitro vs conventional culture method. In results, it seems clear that in vitro re-culture of lily bulblet was able to be sustained and maintained its growth and so, ontogenic development after their performance in soil. Our results demonstrate that in the conventional pathway, the course of bulblet growth to bulb and ontogenic development took 3–4 growing seasons to reach the adult flowering phase. On the other hand, along with the re-culture in vitro, the course of bulb growth and ontogenic development took 1–2 growing seasons to reach the adult flowering phase because of the increasing initial bulb size and advancement of ontogenic development. Other than bulblet production through bulb scale explant, this study represents the first report on the method of in vitro bulb production of lily through re-culture by in vitro pathway with a comparison of the timelines and ontogenic development obtained from in vitro versus conventional pathway.

show abstract

Micropropagation of Flower Bulbs: Lily and Narcissus

Cited by 15 publications

References 7 publications

Phase change in lily bulblets regenerated in vitro

Phase change in lily bulblets regenerated in vitro

Contribution of explant carbohydrate reserves and sucrose in the medium to bulb growth of lily regenerated on scale segments in vitro

Factors Affecting Bulblet Growth of Lilium sp. – A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway

Contact Info

Product

Resources

About

Micropropagation of Flower Bulbs: Lily and Narcissus

Cited by 15 publications

References 7 publications

Phase change in lily bulblets regenerated in vitro

Phase change in lily bulblets regenerated in vitro

Contribution of explant carbohydrate reserves and sucrose in the medium to bulb growth of lily regenerated on scale segments in vitro

Factors Affecting Bulblet Growth of Lilium sp. &ndash; A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway

Contact Info

Product

Resources

About

Factors Affecting Bulblet Growth of Lilium sp. – A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway