Micropropagation of Eurycoma longifolia Jack via Formation of Somatic
Embryogenesis

Sobri, H; Rusli, Iffah Syazana; L.P.K., Anna; Fadzillah, Nor'Aini Mohd; Daud, Siti Khalijah

doi:10.3923/ajps.2005.472.485

Cited by 15 publications

(5 citation statements)

References 27 publications

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“…Aziz et al [ 71 ] induced direct somatic embryos (SEs) and indirect ones (via embryonic calli (EC)) from immature cotyledons on NAA and 2,4-D, respectively. Similarly, Hussein et al [ 72 ], using different explants, concluded that cotyledonary tissues produced EC on 1.0 mg/L 2,4-D, and EC subculture on 0.5 mg/L kinetin plus 1 mg/L 2,4-D resulted in a higher yield. Furthermore, EC were used to induce SEs on liquid media containing 1–2.5 mg/L 2,4-D, 2.0 mg/L BAP, and kinetin [ 73 ].…”

Section: Tissue Culture Techniques For Mass Propagation In ...mentioning

confidence: 97%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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Over the last two decades, there has been a concerted effort by researchers to mass propagate Eurycoma longifolia and improve the yield of its very important and sought-after anti-cancer and aphrodisiac bioactive compounds. To achieve this, various techniques have been used to mass propagate and improve the yield of these bioactive compounds in tissue cultures. These techniques include the optimization of media conditions and application of various types and combinations of plant growth regulators (PGRs). In addition, some elicitation techniques have been used to improve the synthesis of these bioactive compounds. However, in comparison with other herbal species with similar economic importance, many techniques have not been applied to E. longifolia. Adopting the most recent methodologies would ensure efficiency and sustainability in the in vitro production of bioactive compounds in E. longifolia. Therefore, in this review, we present an up-to-date record on the success stories in the tissue culture techniques and synthesis of bioactive compounds. In addition, we attempted to identify some of the missing links on the road to the effective and sustainable biotechnological utilization of this super important biological resource.

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Section: Tissue Culture Techniques For Mass Propagation In ...mentioning

confidence: 97%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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“…The plant usualy found in the tropical forest of Asean countries. All parts of the plant (root, stem, root bark, and leaf) are proved to be medicinal [6]. Due to its benefits, a lot of Tongkat Ali based products are sold in the market.…”

Section: Introductionmentioning

confidence: 99%

“…The most common method of E. longifolia propagation is through seeds. However, like other woody species, propagation of Tongkat Ali through seed is difficult due to the unreliable flowering habit and quick loss of viability as well as low seed germination rate and slow growth [6]. Tissue culture technique producing Tongkat Ali plantlets will provide the solution to encounter the shortage of planting material.…”

Section: Introductionmentioning

confidence: 99%

Acclimatization of Eurycoma longifolia (Tongkat Ali) Plantlets to Ex Vitro Conditions

Yahya

Hassan

Abdullah

et al. 2021

JTRSS

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Eurycoma longifolia is one of the famous herbal plants with great medicinal benefits. The plant which also known as Tongkat Ali is well-known for treating erectile dysfunction and as energy booster. Tongkat Ali plantlets were produced using in vitro propagation technique to cater the demand for planting material. The development of successful acclimatization technique is prerequisite for in vitro propagation method. Acclimatization is a step to prepare the plantlets to survive and grow in different environment compare to laboratory. In this study, an attempt was made to identify the best potting media to acclimatize Tongkat Ali using glass chamber. Rooted plantlets about 2-4 cm height were used and subsequently transferred to different potting media for acclimatization i.e jiffy 7, sand, baked soil, mixture of cocoa peat and sand, sand and top soil and lastly cocoa peat and baked soil. Plantlets grown in jiffy 7 showed 100% survival followed by the mixture of cocoa peat and baked soil with 94.45% of survival. The acclimatized plantlets were transplanted into polybag and maintained in the shadehouse condition.

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“…Although a great number of researches have been done on E. longifolia, micropropagation studies are still scarce and insufficient. Hussein et al (2005a) successfully regenerated shoots from cotyledon explant of E. longifolia through indirect somatic embryogenesis. Mahmood et al (2010) then reported optimizing suitable auxin types and concentrations for induction of callus from different explants of E. longifolia.…”

Section: Introductionmentioning

confidence: 99%

Direct Shoot Organogenesis and Clonal Fidelity Confirmation of Tongkat Ali (Eurycoma longifolia) using Molecular Markers

Alttaher¹

2021

IJAB

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Eurycoma longifolia is a medicinally potent plant found in the tropical forest of South-East Asia. Every part of the plant, especially the root is traditionally used as an aphrodisiac, anticancer and anti-inflammatory. E. longifolia is conventionally propagated by seeds but with low germination rate and efficiency. This has made an in vitro propagation of E. longifolia a desirable alternative. Hence, this study reports an effective method of direct organogenesis of shoot. In vitro seedling’s leaves were cultured on Murashige and Skoog (MS) medium containing 1.0 mg L-1 6-benzylaminopurine (BAP), producing 1.8 ± 0.5 shoots per leaf with a regeneration frequency of 68.2%. The shoot buds were directly formed from leaves without intermediate callus formation. To obtain complete plantlets, the shoots were in vitro rooted with an average number of 4.2 ± 0.4 roots per shoot in half-strength MS (½MS) medium supplemented with 0.5 mg L-1 indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized to field conditions with an 85% survival rate. Genetic fidelity of the micropropagated plantlets was evaluated using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) analysis. The results showed that the monomorphic banding patterns of in vitro raised plantlets and their mother plant were similar, confirming its homogeneity and the reliability of the multiplication system. © 2021 Friends Science Publishers

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Micropropagation of Eurycoma longifolia Jack via Formation of Somatic Embryogenesis

Cited by 15 publications

References 27 publications

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Acclimatization of Eurycoma longifolia (Tongkat Ali) Plantlets to Ex Vitro Conditions

Direct Shoot Organogenesis and Clonal Fidelity Confirmation of Tongkat Ali (Eurycoma longifolia) using Molecular Markers

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