Micropropagation of Elaeocarpus robustus Roxb.

Roy, S. K.; Islam, Md. Saiful; Hadiuzzaman, Syed

doi:10.1007/s002990050488

Cited by 18 publications

(7 citation statements)

References 9 publications

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“…1c). The promoting effect of double cytokinins (BA and Kn) on shoot bud induction from shoot tip and nodal explants were reported previously for a standard-sized Indian olive (Roy et al 1998, Rahman et al 2003a). For axillary shoot regeneration from nodal explants, the supplemented suitable concentration of these cytokinins (BA and Kn) was about two times higher in the dwarf cultivar compared to that for the standard-sized Indian olive.…”

Section: Resultsmentioning

confidence: 75%

“…15% CW, where the shoot regeneration frequency, number of shoots, and length of shoots were 90%, 7.2 ± 0.3, and 5.5 ± 0.2 cm, respectively ( Table 1). The addition of 10% (Roy et al 1998) and 15% CW (Rahman et al 2003a) to the medium resulted in a significant increase in axillary shoot regeneration in the standard-sized Indian olive.…”

Section: Resultsmentioning

confidence: 97%

“…Micropropagation methods, which strictly maintain clonal fidelity, are particularly applicable to this plant. Some reports have been published on the in vitro propagation of standard-sized E. robustus: axillary shoots regenerated from nodal and shoot tip explants (Roy et al 1998;Rahman et al 2003a); and adventitious shoots induced from leaf (Rahman et al 2003b), cotyledon and epicotyl (Rahman et al 2004a, Roy andSinha 2005), and internode (Rahman et al 2004b). However, there is no report on the micropropagation of E. robustus cv.…”

Section: Introductionmentioning

confidence: 97%

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In vitro plantlet regeneration of “dwarf” Indian olive (Elaeocarpus robustus Roxb.): a fruit plant of Bangladesh

Rahman

Amin

Ishiguri

et al. 2009

Plant Biotechnol Rep

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A plantlet regeneration protocol was developed on pot-grown mature plants of Elaeocarpus robustus Roxb. cv. Dwarf from nodal and leaf explants. The best yield of adventitious shoots was achieved from the leaf-derived calli in a modified MS (MMS 1 , half strength of major salts, full strength of minor salts, and vitamins) medium containing 4.0 lM BA ? 4.0 lM Kn ? 0.5 lM NAA ? 15% coconut water (CW). The shoot multiplication rate was amplified about twofold per culture after the addition of 15% CW to the medium. The rate of shoot multiplication reached maximum at the 5th subculture, and it maintained this rate throughout the 3 subsequent subcultures. The best rooting in vitro was investigated by subculturing the microcuttings in an MMS 2 (half strength of both major salts and minor salts and full strength of vitamins) medium containing 1.0 lM IBA in the dark for one initial week at 30°C, followed by subculturing them in a plant-growth regulator (PGR)-free medium in the light. The plantlets raised in vitro were successfully established under ex vitro conditions.

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Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

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In vitro plantlet regeneration of “dwarf” Indian olive (Elaeocarpus robustus Roxb.): a fruit plant of Bangladesh

Rahman

Amin

Ishiguri

et al. 2009

Plant Biotechnol Rep

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“…Rapid increase of micro shoots were observed when the above shoot containing explant was repeatedly subcultured on the same medium. Effect of CW on shoots proliferation was reported by Roy et al (1998) and Roy (2004 and2005) for Elaeocarpus robustus, Smilax zeylania and Gloriosa superba culture, respectively. But in this investigation for further shoot multiplication and growth, addition of 5% and 10% CW in the nutrient medium was not found to be effective.…”

Section: Resultsmentioning

confidence: 77%

<i>In vitro</i> Shoot Proliferation and Plant Regeneration of <i>Physalis minima</i> L. - a Perennial Medicinal Herb

Afroz

Hassan

Bari

et al. 2010

Bangladesh J. Sci. Ind. Res.

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The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate. Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration DOI: 10.3329/bjsir.v44i4.4597 Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

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“…Very limited work has been reported on micropropagation of Elaeocarpus species in India and abroad. There has been mention of in vitro propagation of selective Elaeocarpus species in the past including E. sphaericus (Chauhan et al 2015) and few others such as E. robustus, native olive or 'Jalpai' (Roy et al 1998;Rahman et al 2003) and E. tuberculatus (Arshad and Kumar 2006) whose nuts are used as substitute of Rudraksha (Murti 1993). The present investigation was, thus, undertaken to develop a rapid and efficient in vitro propagation method using nodal explants of E. sphaericus brought from its natural habitat in North East India for large scale production at regions harbouring subtropical climatic conditions suitable for the growth of this plant species.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Rudraksha (Elaeocarpus sphaericus (Gaertn.) K. Schum): a biotechnological approach for conservation

Saklani

Singh

Purohit

et al. 2015

Physiol Mol Biol Plants

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The species Elaeocarpus sphaericus (Rudraksha) is a religious, medicinally important threatened tree of India. An efficient micropropagation protocol has been developed from nodal explants of this plant species collected from north-east India for large scale production of planting material at favourable sites within the country. Best shoot initiation occurred in MS medium supplemented with 2.2μM BA+2.2μM Kn in combination. Addition of Casein Hydrolysate (CH) (100mg/L) increased the shoot number. Microshoots excised and subcultured in 2.0μM BA further enhanced growth and multiplication. The shoot cultures were maintained in this concentration for 2years with subculturing at 6weeks interval. MS medium containing 5.0μM NAA was most effective for rooting. Successfully acclimatized plants (80%) showed normal growth under suitable habitat conditions.

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Micropropagation of Elaeocarpus robustus Roxb.

Cited by 18 publications

References 9 publications

In vitro plantlet regeneration of “dwarf” Indian olive (Elaeocarpus robustus Roxb.): a fruit plant of Bangladesh

In vitro plantlet regeneration of “dwarf” Indian olive (Elaeocarpus robustus Roxb.): a fruit plant of Bangladesh

<i>In vitro</i> Shoot Proliferation and Plant Regeneration of <i>Physalis minima</i> L. - a Perennial Medicinal Herb

In vitro propagation of Rudraksha (Elaeocarpus sphaericus (Gaertn.) K. Schum): a biotechnological approach for conservation

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