Micropropagation of Cucumis spp.

Ziv, Meira

doi:10.1007/978-3-662-07770-2_5

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…Cross-incompatibility and narrow genetic base in cucumber pose limitations to improvement by classical breeding (Ziv 1992). Tissue culture method for production of multiple shoots in cucumber using cotyledon explants was described by Gambley and Dodd (1990).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Direct plant regeneration from cucumber embryonal axis

Ayyappan¹,

Selvaraj²,

Ganapathi³

et al. 2007

Biologia plant.

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Embryonal axis explants from 2-d-old in vitro germinated seeds were used to induce multiple shoot production. The combination of 4.44 µM BA and 1.59 µM NAA in MS medium triggered the initiation of adventitious shoot buds. The explants with shoot buds produced maximum number of shoots (10.6 per explant) in MS medium supplemented with 4.44 µM BA and 0.065 mM L-glutamine in three successive transfers. The elongated shoots were rooted on MS medium with 4.92 µM IBA. Rooted plants were transferred to soil with a survival rate of 65 %.Additional key words: adventitious shoot bud, benzyladenine, Cucumis sativus L., L-glutamine, naphthalene acetic acid. ⎯⎯⎯⎯Cucumber (Cucumis sativus L.) is an important vegetable crop. Cross-incompatibility and narrow genetic base in cucumber pose limitations to improvement by classical breeding (Ziv 1992). Tissue culture method for production of multiple shoots in cucumber using cotyledon explants was described by Gambley and Dodd (1990). A high frequency of variation in regenerated cucumber plants occurs at the callus or somatic embryo stage (Malepszy and Nadolska-Orezyk 1989). In contrast, in the shoots produced by direct regeneration from explants the morphological or physiological variation was much less (Burza and Malepszy 1995, Plader et al. 1998). Therefore, the present protocol was aimed to develop a technique which allows the production of multiple shoots directly from the embryonal axis of cucumber.Seeds of cucumber (Cucumis sativus L.) cv. Poinsett 76 (Indo-American hybrid seeds, Pvt Ltd, Bangalore, India) were soaked in tap water for 15 min disinfected with 70 % alcohol for 1 min and 2.5 % (v/v) commercial bleach Teepol (5.25 % sodium hypochlorite; ) for 15 min followed by three rinses with sterile distilled water. Seeds were further disinfected by soaking in 0.5 % mercuric chloride (m/v) for 3 min and rinsed four times with sterile distilled water. The axenic seeds were inoculated in sterile culture tubes (25 × 150 mm) containing moist cotton and kept in the culture room. The embryonal axis was removed from germinating seeds at different growth periods under sterile conditions and was used as the explant. Intact embryonal axes without the radical region, embryonal axes without apical and radical regions, and embryonal axes in which a cut was made at the apical region without the radical were cultured on Murashige and Skoog's (1962) (MS) medium supplemented with N 6 -benzyladenine (BA; 0 -8.88 µM), kinetin (KIN; 0 -9.28 µM) individually and in combination with -naphthaleneacetic acid (NAA) at different concentrations (0.53 -2.65 µM) (shoot induction medium). All the media contained 3 % sucrose and were gelled with 0.8 % Phytagel (m/v) (Himedia Laboratories, Mumbai, India). Plant growth regulators were added to the media under sterile conditions and the pH was adjusted to 5.8 before autoclaving at 1.06 kg cm -2 (121 ºC) for 20 min. MS medium without growth regulators served as control. Each culture tube containing 20 cm 3 of one of the above concentrations or combinations was ...

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Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Direct plant regeneration from cucumber embryonal axis

Ayyappan¹,

Selvaraj²,

Ganapathi³

et al. 2007

Biologia plant.

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“…Conventional breeding of cucumber has produced many cultivars, which can be grown under various environmental conditions (Tabei 1997). Conventional breeding to transfer genes between different species within the Cucurbitaceae is difficult due to strong sexual incompatibility barriers at the interspecific and intergeneric levels (Deakin et al 1971;Esquinas-Alcazer and Gulick 1983;Ziv 1992). Conventional breeding to transfer genes between different species within the Cucurbitaceae is difficult due to strong sexual incompatibility barriers at the interspecific and intergeneric levels (Deakin et al 1971;Esquinas-Alcazer and Gulick 1983;Ziv 1992).…”

Section: Introductionmentioning

confidence: 99%

High-frequency in vitro shoot regeneration in Cucumis sativus by inhibition of endogenous auxin

Shukla

Das

Jha

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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Direct in vitro plantlet regeneration was studied in cucumber (Cucumis sativus), an important vegetable crop. The endogenous auxin concentration was quantified by HPLC in young cotyledon explants, and we observed that higher auxin content promotes callusing and inhibits direct shoot regeneration. Lowering the endogenous auxin concentration by the application of the auxin inhibitor 2,3,5triiodobenzoic acid (TIBA), as confirmed by HPLC, increased the frequency of shoot regeneration. The auxin content was reduced by increasing the concentration of TIBA in cvs. Pant Kheera 1 and Pusa Uday. Regeneration efficiencies were dependent on explant age and cultivar. The 2-d-old explants gave the highest regeneration efficiencies, with Pusa Uday showing higher regeneration potential than PK-1. Shoot buds/small shoots transferred to Murashige and Skoog (MS) medium supplemented with 1 μM 6-benzylaminopurine grew vigorously and attained 1-to 2-cm length in 4 wk. These shoots rooted with 100% efficiency on MS basal medium or MS basal medium supplemented with 0.5 μM indole-3-acetic acid. The hardened plants showed normal growth and flowering under greenhouse conditions.

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“…The regeneration of cu-cumber plants has been reported either directly on various explants or via callus that developed on the explant (Ziv 1992). The regeneration of cu-cumber plants has been reported either directly on various explants or via callus that developed on the explant (Ziv 1992).…”

Section: Introductionmentioning

confidence: 99%

“…The regeneration of cu-cumber plants has been reported either directly on various explants or via callus that developed on the explant (Ziv 1992). Upon their transfer to soil, regenerated plants have been successfully acclimatized with a success rate of around 80% (Ziv 1992). Micropropagation is usually via embryogenesis, although organogenesis is also quite common.…”

Section: Introductionmentioning

confidence: 99%

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Developing a scale-up system for the micropropagation of cucumber (Cucumis sativus L.): the effect of growth retardants, liquid culture and vessel size

Konstas

Kintzios

2003

Plant Cell Rep

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We investigated the effect of the growth retardant flurprimidol, the phase of the culture medium (solid versus liquid) and the size of the liquid culture vessel (250-ml flask versus 2.5-l airlift bioreactor) on the micropropagation of cucumber (Cucumis sativus L.) from nodal explants. Flurprimidol at concentrations of 0.1-2 mg l -1 caused considerable growth retardation but increased, albeit slightly, the number of branches and buds and stimulated (solid medium) or reduced (liquid medium) the accumulation of NO 3 −and PO 4 3-. Flurprimidol had varying effects on the accumulation of soluble sugars and antioxidant compounds. Bioreactor-derived plants showed an increased fresh weight and size but a decreased content of macronutrients, solid sugars, ascorbic acid and free antioxidant phenolics. The majority (95%) of the plants were successfully acclimatized after being graft on squash. The perspective for an efficient, commercial-level use of bioreactors in combination with growth retardants of this commercially important vegetable species is discussed.

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Micropropagation of Cucumis spp.

Cited by 5 publications

References 21 publications

Direct plant regeneration from cucumber embryonal axis

Direct plant regeneration from cucumber embryonal axis

High-frequency in vitro shoot regeneration in Cucumis sativus by inhibition of endogenous auxin

Developing a scale-up system for the micropropagation of cucumber (Cucumis sativus L.): the effect of growth retardants, liquid culture and vessel size

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