Micropropagation of commercial and wild genotypes of snapdragon (<i>Antirrhinum</i>spp.)

González‐Benito, M. Elena; Tapia, José Luis; Rodríguez, Novisel Veitía; Iriondo, J. M.

doi:10.1080/14620316.1996.11515377

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…Thus, at BA concentrations above 4.44 µM hypocotyls responded unfavorably. Number of shoots per hypocotyl in our study exceeded previous studies by up to 5-fold, probably because younger hypocotyls (Atkinson et al, 1989;Gonzalez-Benito et al, 1996) and a different plant growth regulator (Atkinson et al, 1989) were used.…”

Section: Resultscontrasting

confidence: 67%

“…Lack of a good adventitious shoot regeneration system has hindered such advancement. Varying success with micropropagation of A. majus has been achieved using explants from internodes, nodes, shoot tips, and roots (Atkinson et al, 1989;Gonzalez-Benito et al, 1996;Pfister and Widholm, 1984;Sangwan and Harada, 1975); best results were obtained with explants from seedling shoot tips or hypocotyls (Atkinson et al, 1989;Gonzalez-Benito et al, 1996;Okubo et al, 1991;Sangwan and Sangwan, 1990).…”

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confidence: 99%

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Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro

Schroeder¹,

Stimart²

1999

HortSci

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Hypocotyls from Antirrhinum majus L. were excised at 2 weeks of age from seedlings grown under a 16-hour photoperiod or continuous darkness. Explants were cultured on modified Murashige-Skoog (MS) medium containing 0, 0.44, 2.22, 4.44, 8.88, or 44.4 μm BA to investigate adventitious shoot formation. Excised hypocotyls from eight commercial cultivars, three inbred lines, and an F1 hybrid between two of the inbreds were cultured on MS medium containing 2.22 μm BA to assess genotypic effects on adventitious shoot formation. The influence of seedling age was assessed by excising hypocotyls from seedlings at 6, 10, 14, 18, 22, 26, or 30 days. Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment, use of hypocotyls from 10-day-old seedlings, and culture on medium containing 2.22 μm BA for 3 weeks. Under these conditions, up to a 5-fold improvement in number of shoots per hypocotyl over previous studies was achieved. Adventitious shoot formation was genotype-dependent and appeared to be a dominant trait. Chemical name used: N6-benzyladenine (BA).

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Section: Resultscontrasting

confidence: 67%

mentioning

confidence: 99%

Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro

Schroeder¹,

Stimart²

1999

HortSci

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“…In A . majus , it has been reported that the presence of cytokinins (kinetin or BAP) in the medium seems to be favorable for multiple shoot formation [ 51 , 52 ], but high concentrations of growth regulators used individually or in combination usually lead to the formation of callus with occasional abnormal shooting [ 53 ]. For A .…”

Section: Resultsmentioning

confidence: 99%

Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation

et al. 2013

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Plant phytochemicals are described as possessing considerable neuroprotective properties, due to radical scavenging capacity and acetylcholinesterase inhibitory activity, important bioactivities in neurodegeneration. Antirrhinum lopesianum is a rare endemism from the Iberian Peninsula, occurring at the northeastern border between Portugal and Spain. It is classified as Endangered, due to its highly fragmented geographical occupation, facing a high risk of extinction in the Portuguese territory, within 20 years. Here, we describe for the first time the chemical characterization of extracts of the species concerning total phenol content, flavonoid content and antioxidant properties. The profile of high performance liquid chromatography with diode array detector (HPLC-DAD) of the polyphenol-enriched fraction of plant extracts was also performed, showing the great potential of the species as a source of bioactive phytochemical compounds. A. lopesianum’s potential for neuroprotection was revealed by a significant acetylcholinesterase inhibitory activity and also by a neuroprotective effect on a human cell model of neurodegeneration. Moreover, this is the first report describing a successful procedure for the in vitro propagation of this endangered species. The comparison of phenolic content and the HPLC-DAD profile of wild and in vitro propagated plants revealed that in vitro plants maintain the ability to produce secondary metabolites, but the profiles are differentially affected by the growth regulators. The results presented here greatly contribute to the value for this species regarding its potential as a source of phytochemicals with prospective neuroprotective health benefits.

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“…This characteristic of micropropagation is of special interest when buds or Lledó et al (1996) Scrophulariaceae Antirrhinum microphyllum Roth. González-Benito et al (1996) Daphne rodriguezii Teixidor Estades and Medrano (1990) IUCN categories: CR critically endangered, EN endangered, VU vulnerable a IUCN Red List category in Bañares et al 2004. b In these references, it is mentioned that in vitro culture of the species was studied although no direct reference was found nodal segments can be obtained from individuals of perennial species, and hence, with minimal impact on the native population. In some Spanish endangered species, the use of nodal segments from field-grown plants has allowed the use of adult material.…”

Section: Uses and Futurementioning

confidence: 99%

In Vitro Preservation of Spanish Biodiversity

González‐Benito

Martı́n

2010

In Vitro Cell.Dev.Biol.-Plant

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Spanish territories contain many of the hot spots of plant biodiversity among European countries. Most of the Spanish territory is found in the Mediterranean basin and in the Canary Islands, a region of great floral singularity and diversity (Macaronesian bioregion). Therefore, an important effort must be made to contribute to its conservation. Several strategies can be considered, but seed conservation under standard conditions is the most resource-efficient method. However, the application of this methodology is not always possible for recalcitrant seeds or species for which vegetative propagation is necessary or convenient under some circumstances. Micropropagation is one of the measures suggested for preserving endangered species. During the 1990s, several in vitro culture protocols for Spanish endemics were established. The main purpose of this strategy was to obtain a considerable number of individuals to reduce the loss of natural populations. Likewise, diverse slow growth protocols were developed for this material. However, these efforts usually did not lead to the establishment of in vitro collections. The advantages and disadvantages of the in vitro conservation strategy will be reviewed for some cases. The establishment of the in vitro protocols together with the development of cryopreservation techniques created the ideal conditions to generate cryogenic collections. In this paper, we review the knowledge and experience accumulated during the last decades in micropropagation, slow growth, and cryopreservation for Spanish plant wild species. Their application in the development ex situ collections and their contribution toward an integrated system to conserve threatened species will be discussed.

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Micropropagation of commercial and wild genotypes of snapdragon (Antirrhinumspp.)

Cited by 5 publications

References 6 publications

Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro

Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro

Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation

In Vitro Preservation of Spanish Biodiversity

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