Micropropagation of clones from controlled crosses of Gmelina arborea in Costa Rica

Valverde-Cerdas, Lisette; Alvarado, Laura; Gómez, A.

doi:10.1023/b:nefo.0000040945.75019.38

Cited by 5 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Sul and Korban 14 , Meneguzzi et al 18 , Valverde et al 25 and Montalbán et al 26 in forestry species, BAP stimulated the axillary bud breakage and shoot elongation. In our study, a similar result was obtained in the BAP treatment.…”

Section: Discussionmentioning

confidence: 93%

Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Rojas-Vargas

Castander-Olarieta²,

Moncaleán³

et al. 2021

View full text Add to dashboard Cite

Forest trees are renewable sources of timber and other valuable non-timber products. Nowadays, the increase in population and demand for forestry products results in overexploitation of forestry. Therefore, there is an urgent need to produce elite plants with higher productivity under stress derived from climate change to have available to afforestation. For this reason, propagation methods should be improved to be more efficient in terms of quality and productivity. The main species planted in the Basque Country is Pinus radiata; during the last three years, Pinus radiata plantations have suffered a fungus attack affecting mainly needles until the tree's death. This crisis is caused by the combined action of two fungi of the genus Dothistroma and Lecanosticta acicola, whose expansion seems to have been enhanced by weather-related factors, such as humid and hot summers. Although we have evidence of this disease's presence in our mountains since 1942, the disease has had a speedy expansion with an aggressive effect for reasons that are not scientifically known today. For the above, Basque Country forestry sector is looking for alternative species to be used in its plantations. Part of the forestry sector considers that Sequoia sempervirens could be a good choice for plantations. Besides, its high-quality wood and its tolerance to the attack of several pathogens and other diseases derived from climate change are characteristics that could confer some advantage over other forest species. The main goal of this study was to optimize the micropropagation of adult elite trees of Sequoia sempervirens. The effect of 6-benzylaminopurine, meta-Topolin and Kinetin, and 4 types of explant in the multiplication stage were analyzed to carry out this objective. Furthermore, the effect of two types of auxins: 1-naphthalene acetic acid, indole-3-butyric acid, and a mixture of both, were evaluated on the induction of roots and their subsequent effect on the acclimatization process. The best multiplication index was obtained when 4.4 µM 6-benzylaminopurine and apical explants longer than 1.5 cm of length were used. The root induction percentage was 75% in the most responsive genotype analyzed when 4.4 µM 6-benzylaminopurine was used on the induction stage, and 50 µM of 1-naphthalene acetic acid was used for rooting. Finally, after 3 months in the greenhouse, the explants cultured with Kinetin and rooted in a culture medium with indole-3-butyric acid showed the highest acclimatization success (94%).

show abstract

Section: Discussionmentioning

confidence: 93%

Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Rojas-Vargas

Castander-Olarieta²,

Moncaleán³

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Twenty plantlets with a taproot were used with an average height of 2.0 cm. In the greenhouse, in vitro plants were removed from the culture dish using the protocol established by Valverde et al ( 2004) [7]. The plant length (cm) and percentage survival was evaluated.…”

Section: Greenhouse Acclimatization and Field Growthmentioning

confidence: 99%

In Vitro Culture of the New Anxiolytic Plant, Souroubea Sympetala

Rojas-Vargas

Gómez

et al. 2020

J NHP Res

View full text Add to dashboard Cite

INTRODUCTION:A protocol for the in vitro culture of the anxiolytic medicinal plant Souroubea sympetala (Marcgraviaceae) was developed, representing one of the first in vitro cultures for the family. This species was previously very difficult to cultivate from seed or cuttings. METHODS:Methods included (1) the improvement of seed germination by axenic culture (2) development of regenerative cultures in vitro, then cultivation under greenhouse and finally field conditions and (3) creation of cell suspensions. Phytochemical analysis was undertaken by liquid chromatography coupled to mass spectrometry (HPLC-MS). RESULTS:The percentage of seed germination was improved from 2% to 59% in axenic culture and the full development of the seedling with its apical shoot and root took twenty-four days. The best seedling development was obtained in Gamborg B5 culture medium. Most friable callus formation, (66.7%) was obtained in the Murashige and Skoog medium supplemented with naphthalene acetic acid (1 mg • L -1 ) and kinetin (0.5 mg • L -1 ) from which viable cell cultures were developed. Analysis identified 4 main triterpenes with both in vitro plants and greenhouse grown plants derived from them. The triterpenes were betulinic acid, ursolic acid, alpha-amyrin and beta-amyrin. The betulinic acid found in greenhouse plants was comparable to wild plants. The cell suspension cultures had much lower levels of betulinic acid than plants and are not at present a viable source of this anxiolytic triterpene. DISCUSSION: The improvement in seed germination of this recalcitrant tropical species was highly successful. The subsequent in vitro propagation and progression of plants through greenhouse and field conditions to provide mature plants with active principle concentrations comparable to wild plants was promising. Friable callus was achieved but phytochemical analysis showed that the level of betulinic acid in callus was much lower than that found in mature plant tissue. CONCLUSION:The method provides healthy plants for cultivation of this new medicinal plant and consequently harvesting of wild plants is not required.

show abstract