Micropropagation of chestnut rose (<i>Rosa roxburghii</i>Tratt) and assessment of genetic stability in<i>in vitro</i>plants using RAPD and AFLP markers

Wen, Xiaopeng; Deng, Xiuxin

doi:10.1080/14620316.2005.11511891

Cited by 19 publications

(8 citation statements)

References 27 publications

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“…Based on published reports on occurrence of somaclonal variation (Zucchi et al, 2002;Wen et al, 2005;Ngezahayo et al, 2006) among plants grown through enhanced axillary branching cultures, we estimated genetic stability of plum shoots before and after manipulations in vitro. AFLP analysis showed that genome instability in vitro can be detected in P. domestica cultivars (Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Influence of Ribavirin on Prunus Domestica L. Regeneration, Genome Stability and Virus Eradication In Vitro

Mažeikienė

Kviklys

Šikšnianienė

et al. 2019

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Prunus necrotic ring spot ilarvirus (PNRSV) and Apple chlorotic leaf spot trichovirus (ACLSV) are common in plum orchards. The aim of the study was to obtain virus-free planting material of Prunus domestica L. by chemotherapy in vitro. Ribavirin at concentrations of 10 to 50 mg·l−1 was added to Murashige–Skoog (MS) nutrition medium for virus eradication from microshoots. After a two-week period of chemotherapy, meristems were subcultured monthly on MS medium and proliferation index of shoots was estimated. Microshoots were retested by reverse transcription polymerase chain reaction for presence of virus. At lowest concentrations of 10 mg·l−1 ribavirin was entirely ineffective for ACLSV and 10 to 30 mg·l−1 was ineffective for PNRSV elimination. Ribavirin concentrations of 40 and 50 mg·l−1 destroyed both pathogens. However, at higher concentrations of 40 and 50 mg·l−1 ribavirin exhibited some signs of phytotoxicity on microshoots in the first sub-cultivation period. In order to test the genetic stability of the microplants after chemotherapy the amplified fragment length polymorphism (AFLP) method was applied. Plant genome stability in ‘Magna Glauca’ at concentrations of 40 mg·l−1 was damaged, as the presence of polymorphic AFLP markers were observed.

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Section: Discussionmentioning

confidence: 99%

“…AFLP markers have the potential to resolve genetic differences of DNA fingerprints (Ulrich et al, 1999). AFLP studies have shown an insufficient genetic stability in some plant populations using AFLP (Wen et al 2005;Tiwari et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Influence of Ribavirin on Prunus Domestica L. Regeneration, Genome Stability and Virus Eradication In Vitro

Mažeikienė

Kviklys

Šikšnianienė

et al. 2019

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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“…Low survival and shoot formation in M. laevigata Wall, M. serrata Roxb, M. tiliaefolia Makino, M. rubra L, M. rotundiloba Koidz, and Morus cathayana Hemsl indicate a need for modification of culturing conditions (Pattnaik and Chand 1997;Wen and Deng 2005). Inclusion of low concentration of auxins (0.2 to 0.5 mg l −1 Naphthalene acetic acid (NAA) or GA 3 ) along with cytokinin BA (1 mg l −1 ) in the MS medium enhanced in vitro shoot formation after cryopreservation in many of the accessions (Khurana et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Mulberry biodiversity conservation through cryopreservation

Atmakuri

Chaudhury

Malik

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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A 238 mulberry germplasm accession collection from diverse regions maintained under tropical conditions was identified from an ex situ field gene bank. The purpose was to prioritize the in vitro conservation and cryopreservation to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Reliable cryo techniques using desiccation and slow freezing of winter-dormant buds were used. Storage potential of bud grafts of different Morus species at −1.5°C for 90 d indicated species-specific variation, and most of the wild species were found sensitive. In vitro regeneration and cryopreservation (−196°C) protocols using differentiated bud meristems, like axillary winter-dormant buds, were worked out for a wide range of landraces, wild, and cultivated varieties of Morus. Buds maintained under subtropical location are also amenable for cryopreservation. Successful cryopreservation of winter-dormant buds belonging to Morus indica, Morus alba, Morus latifolia, Morus cathayana, Morus laevigata, Morus nigra, Morus australis, Morus bombycis, Morus sinensis, Morus multicaulis, and Morus rotundiloba was achieved. Among wild species, Morus tiliaefolia and Morus serrata showed moderate recovery after cryopreservation. Survival rates did not alter after 3 yr of cryopreservation. Inter-simple sequence repeat markers were used to ascertain the genetic stability of cryopreserved mulberry germplasm accessions, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the nonfrozen material.

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“…Plant breeders have shown interest in chestnut rose as a new fruit crop over the past two decades (Fan et al, 1997;Ma et al, 1997;Wen and Deng, 2005) because of its beneficial nutritional and medicinal properties. R. roxburghii fruits are also of economic importance for making beverages and wine (Fan et al, 1997).…”

mentioning

confidence: 98%

“…Chestnut rose has been the subject of few in vitro studies compared with other species (Wen and Deng, 2005). Among the documented studies of this genus, little information exists concerning regeneration from immature zygotic embryos (Rout et al, 1999).…”

mentioning

confidence: 99%

Plant regeneration from immature zygotic embryos of chestnut rose (Rosa roxburghiiTratt) through organogenesis

Wen

Deng

2005

The Journal of Horticultural Science and Biotechnology

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Micropropagation of chestnut rose (Rosa roxburghiiTratt) and assessment of genetic stability inin vitroplants using RAPD and AFLP markers

Cited by 19 publications

References 27 publications

Influence of Ribavirin on Prunus Domestica L. Regeneration, Genome Stability and Virus Eradication In Vitro

Influence of Ribavirin on Prunus Domestica L. Regeneration, Genome Stability and Virus Eradication In Vitro

Mulberry biodiversity conservation through cryopreservation

Plant regeneration from immature zygotic embryos of chestnut rose (Rosa roxburghiiTratt) through organogenesis

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