Micropropagation of Black Turmeric (Curcuma Caesia Roxb.) Through in Vitro Culture of Rhizome Bud Explants

Abstract

In the present study, preliminary in vitro shoots propagation of Curcuma caesia Roxb. was investigated. Rhizome buds were used as explants and were cultured on Murashige and Skoog (MS) medium containing 6-Benzyl adenine (BA) alone or in combination with α-Naphthalene acetic acid (NAA). The results showed that the optimum shoot proliferation was obtained from MS medium containing 3.0 µM BA + 0.5 µM NAA. In this growth regulator combination, maximum 99.97 % explants produced 10.38 shoots with 4.53 cm length after 8 weeks of culture. Although spontaneous rooting was observed after 4 weeks of cultivation with all treatments using half strength MS medium containing IBA and NAA at different concentrations, high frequency of rooting (89.76 %) was obtained in 3.0 µM IBA (Indole-3-butyric acid) containing medium. The plantlets, thus developed, were hardened and successfully established in natural soil.

Conservation and In Vitro Propagation of an Endangered Wild Turmeric (Curcuma caesia Roxb.) Species from Sub - Himalayan Terai Region of West Bengal

“…According to Shahinozzaman et al, (2013) the optimum shoot proliferation was found in case of black turmeric from MS medium containing 3.0 μM BA + 0.5 μM NAA and produced highest shoots with 4.53 cm length after 8 weeks of culture.…”

Micropropagation of <i>Curcuma Sp</i>., a Threatened Medicinal Plant

“…Used the result of micropropagation of the species from the same Curcuma genus: C. longa [4] [5], C. zedoaria [6], C. amarrotica [7], C. caesia [8] [9], C. mangga [10]- [12], use medium MS (compele-100%) after 4, 6, 8 weeks, the result is not good: samples not division cell, could not form callus. Therefore, we have to test with 5 mediums as Table 1 in order to choose a suitable medium to cell of Curcuma sp.…”

Abiotic stresses increase plant regeneration ability of rhizome explants of Curcuma caesia Roxb.

“…About 20 % of the yield has to be utilized for raising the next generation and the seed rhizome needs careful preservation from pathogenic organisms especially bacteria, fungi and insects (Balachandran et al 1990;Shirgurkar et al 2001). Micropropagation of C. caesia using rhizome bud and rhizome piece explants has been investigated (Bharalee et al 2005;Shahinozzaman et al 2013). However, the efficiency of shoot induction was comparatively low (1.4-10.38 shoots per explant).…”