Micropropagation of Anthurium spp.

Atak, Çimen; Çelik, Özge

doi:10.5772/51426

Cited by 9 publications

(9 citation statements)

References 23 publications

(12 reference statements)

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“…cultivars on SMRM Nhut et al, (2006). Our results are in line with earlier report (Atak and Celik, 2009). The reason may be that, many genotype dependent effects are caused by interactions between the plant's genotype and the cultural environment (George, et al, 2008).…”

Section: Resultssupporting

confidence: 83%

“…The average callus multiplication ratio ranged from 2.61-4.40 times in different cultivars (Table 1). In earlier studies number of sub cultures was limited to four (Atak and Celik, 2009;Gantait et al, 2008). In this study, subsequently, shoot bud formation and elongation was observed in callus cultures incubated in dark for 40-45 days or more in CISIM.…”

Section: Resultsmentioning

confidence: 99%

“…Tissue culture offers an alternative tool for rapid multiplication quality planting material in short period. In vitro plant propagation in Anthurium has been achieved by using various tissues including leaf, petiole, spadix, spathe, seed, lateral bud and shoot tips through direct and indirect organogenesis (Atak and Celik, 2009) and this technique is commercially used (Martin et al, 2003) for production of planting material. Further, various physical and biological factors including media play role during Anthurium micro propagation (Silva et al, 2005;Bejoy et al, 2008) and one of the important factors among them was genotype.…”

Section: Issn: 2319-7706 Volume 6 Number 9 (2017) Pp 2579-2584mentioning

confidence: 99%

See 2 more Smart Citations

In Vitro Mass Multiplication of Anthurium andreanum (HORT) Cultivars

Prakasha¹,

Ramya²,

Srinivasalu³

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Issn: 2319-7706 Volume 6 Number 9 (2017) Pp 2579-2584mentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Mass Multiplication of Anthurium andreanum (HORT) Cultivars

Prakasha¹,

Ramya²,

Srinivasalu³

2017

Int.J.Curr.Microbiol.App.Sci

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“…The propagation aspects of tissue culture methods were previously covered by Gantait and Mandal (2010) and Atak and Ç elik (2012). However, these reviews failed to report details of important in vitro conditions essential for successful growth and development and did not discuss the developed tissue culture methods of importance to breeding, such as anther culture or polyploidy production.…”

Section: Why Are In Vitro Methods Important In Anthurium Breeding Andmentioning

confidence: 95%

Anthurium in vitro: A review

Silva¹,

Dobránszki

Winarto³

et al. 2015

Scientia Horticulturae

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“…Micropropagation of Anthurium cultivars through leaf explant culture and callus induction and subsequent plantlet regeneration is widely adopted for mass production worldwide (Kuehnle and Sugii, 1991;Martin et al, 2003;Teng, 1997). The first important step in micropropagation is to establish an aseptic plant material culture in order to eliminate bacterial, fungal, and insect contaminants effectively (Atak and Özae, 2012;Chen and Yeh, 2007;Fang and Hsu, 2012;Gantait and Mandal, 2010). However, surface disinfection to establish clean, aseptic…”

Section: Introductionmentioning

confidence: 99%

Establishment of an Efficient Micropropagation System in <i>Anthurium</i> Hybrids Through <i>In Vitro</i> Callogenesis and Suspension Culture

2020

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The development of new Anthurium cultivars relies on efficient micropropagation, which is highly dependent on genotypes, to provide enough young plants for cultivation. Microbial contamination is the critical factor for successful initiation of aseptic culture of Anthurium cultivars. The aims of this study were to investigate surface disinfection with either sodium hypochlorite or a fungicide Plant Preservative Mixture TM (PPM), and supplemental PPM in the medium to evaluate their effect on reducing contamination and tissue browning. Next, a liquid suspension culture system was developed to regenerate the adventitious shoots efficiently in a short time for subsequent establishment of young plantlets for transplanting. Disinfecting newly developed leaves of 'Kaohsiung No. 1' with 0.6% sodium hypochlorite was more effective than other disinfection treatments even though only 5% of leaf explants produced callus. However, disinfecting the leaves of 'Kaohsiung No. 2' with 0.15% sodium hypochlorite was more effective than other treatments, with a callus induction rate up to 100%. No callus was induced on 'Orange Hot' at any hypochlorite concentration. Disinfection using PPM could reduce the contamination rate. By supplementing 0.01% PPM to the culture medium, the callus induction rate of 'Kaohsiung No. 2' was up to 55%, and the browning rate was lower than the control with hypochlorite disinfection. However, surface disinfection using 25% and 50% PPM did not lead to any callus formation in any of the three cultivars. Calluses of A. 'Kaohsiung No. 2' induced after 60 days of culture were transferred onto liquid suspension after 60 days for further proliferation of adventitious shoots and subsequent plantlet regeneration. Young plantlets could be successfully transplanted in peat moss and later flowered within 16 months.

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Micropropagation of Anthurium spp.

Cited by 9 publications

References 23 publications

In Vitro Mass Multiplication of Anthurium andreanum (HORT) Cultivars

In Vitro Mass Multiplication of Anthurium andreanum (HORT) Cultivars

Anthurium in vitro: A review

Establishment of an Efficient Micropropagation System in <i>Anthurium</i> Hybrids Through <i>In Vitro</i> Callogenesis and Suspension Culture

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