Micropropagation of a tropical fruit tree Spondias mangifera Willd. through direct organogenesis

Tripathi, M. K.; Kumari, Nishi

doi:10.1007/s11738-010-0484-z

Cited by 34 publications

(18 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was observed that repeated or successive transfer of explants on this medium increased the number of shoots to 17.2 from 4.2 after 2-3 transfers ( Table 1). The increase in shoot number may be due to suppression of apical dominance during subculture that induced basal dormant meristametic cells to form new shoots (Tripathi and Kumari 2010).…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture

Shekhawat

2010

Acta Physiol Plant

View full text Add to dashboard Cite

Alkannin, a red-purple dye and bioactive compound found in the roots of Arnebia hispidissima has antibiotic and anti-inflammatory properties and is also used in cosmetic and textile industries at a large-scale. In the present communication, we demonstrate the establishment of callus and cell suspension culture of A. hispidissima with the aim of optimizing the production of alkannin. Highest alkannin content was recorded in cell suspension and callus culture established on M-9 medium. Production of alkannin was influenced by the different culture medium. Evaluation of alkannin content of roots of field-grown plants and in vitro grown cell, tissue and organ showed that alkannin production was higher in all in vitro grown culture systems (cell suspension, callus and roots) than the roots of fieldgrown plants. The present investigation may be applicable in designing systems for the large-scale cultivation of A. hispidissima cell suspensions for the production of alkannin.

show abstract

Section: Resultsmentioning

confidence: 99%

Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture

Shekhawat

2010

Acta Physiol Plant

View full text Add to dashboard Cite

show abstract

“…1b). The increase in shoot number due to repeated transfer of mother explants may be owing to suppression of apical dominance during subculture which induced basal dominant meristematic cells to form new shoots (Tripathi and Kumari 2010;Shekhawat and Shekhawat 2011). This approach has also been reported in the micropropagation of a number of woody trees (Tripathi and Kumari 2010;Phulwaria et al 2011).…”

Section: Repeated Transfer Of the Mother Explantmentioning

confidence: 89%

Micropropagation of mature Terminalia catappa (Indian Almond), a medicinally important forest tree

Phulwaria

Ram

Harish

et al. 2012

Journal of Forest Research

View full text Add to dashboard Cite

We report an efficient in vitro propagation method for Terminalia catappa using nodal segments of a 15-year-old mature tree. The nodal segments were cultured on MS medium supplemented with 6-benzyladenine (BA; 0.5-3.0 mg l -1 ) or Kinetin (Kn; 0.5-3.0 mg l -1 ) for bud breaking and multiple shoot induction. About 85% of the explant responded (2.8 ± 0.41 shoots per node with 2.7 ± 0.14 cm length) within 15 days of inoculation in Murashige and Skoog medium fortified with 2.0 mg l -1 of BA. Further shoot multiplication was achieved by repeated transfer of mother explants and subculturing of in vitroproduced shoots on medium supplemented with various concentrations of BA (0.25-1.5 mg l -1 ) or Kn (0.25-1.5 mg l -1 ) or on their combinations. Optimal number of shoots and shoot length were recorded on MS medium supplemented with 0.25 mg l -1 of BA and 0.25 mg l -1 of Kn. The multiplied shoots were used for ex vitro rooting after treatment for 4 min with indole-3-butyric acid (IBA; 50-500 mg l -1 ) or a-naphthalene acetic acid (NAA; 50-500 mg l -1 ). About 80% of the shoots treated with 200 mg l -1 of IBA produced ex vitro roots with an average of 2.8 roots per shoot. Nearly 75% of these plantlets could be acclimatized within 5 weeks and successfully established in the field. This is the first report on micropropagation of T. catappa, which can be applied for further genetic transformation assays and pharmaceutical purposes.

show abstract

“…The number of shoots increased on every transfer, reaching the maximum on fourth passage. Tripathi and Kumari (2010) also suggested that the repeated transfer of mother explants suppresses apical dominance allowing induction of basal meristematic cells to form multiple shoots. MS medium supplemented with the (Fig.…”

Section: Effect Of Plant Growth Regulatorsmentioning

confidence: 99%

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Vibha

Shekhawat

Mehandru

et al. 2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

An efficient and improved method for in vitro propagation of mature tree of Dalbergia sissoo, an ecologically and commercially important timber yielding species, has been developed through axillary shoot proliferation. Bud breaking occurred from nodal shoot segments derived from rejuvenated shoots produced during early spring from a 20-25-year-old lopped tree, on MS medium containing 8.88 μM benzylaminopurine (BAP). Multiple shoots differentiated (20-21shoots/node) on re-culture of explants on halfstrength agar gelled amended MS medium with a combination of 2.22 μM of BAP and 0.002 μM of thidiazuron (TDZ) with 1.0 mM each of Ca(NO 3 ) 2 , K 2 SO 4 , KCl, and NH 4 (SO 4 ) 2 . The maximum shoot multiplication (29-30 shoots/node) was achieved on subculturing in the above mentioned but liquid medium. Furthermore, the problem of shoot tip necrosis and defoliation observed on solid medium were overcome by the use of liquid medium. Ex vitro rooting was achieved on soilrite after basal treatment of microshoots with 984 μM of indole-3-butyric acid (IBA) for 2 min. About 90 % microshoots were rooted on soilrite within 2-3 weeks under the greenhouse conditions. From 20 nodal shoot segments, about 435 hardened plants were acclimatized and transplanted. This is the first report for rapid in vitro propagation of mature trees of D. sissoo on liquid medium followed by ex vitro rooting.

show abstract

Micropropagation of a tropical fruit tree Spondias mangifera Willd. through direct organogenesis

Cited by 34 publications

References 23 publications

Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture

Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture

Micropropagation of mature Terminalia catappa (Indian Almond), a medicinally important forest tree

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Contact Info

Product

Resources

About