Micropropagation, encapsulation, and conservation of Decalepis salicifolia, a vanillin isomer containing medicinal and aromatic plant

In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

“…This technique has been used in rare plant conservation strategies and has been studied for ex situ conservation of threatened species in many plant families (Sheelavantmath et al 2000;Ayuso et al 2019;Rodrigues et al 2020). Passi ora xishuangbannaensis growth was shown to be enhanced by grafting on P. edulis Sims.…”

“…These alterations might be due to mutagenesis during the breakdown of normal cellular controls (Phillips et al1994), or accumulation of somatic mutations induced by several factors including the various concentrations and types of PGRs used during the tissue culture period (Martin et al 2006;Bairu et al 2011). Molecular markers, such as Simple Sequence Repeat (SSR), Inter Simple Sequence Repeat (ISSR), Random Ampli ed Polymorphic DNA (RAPD), Start Codon Targeted (SCoT) polymorphism, Inter-retrotransposon Ampli ed Polymorphism (IRAP), and Ampli ed Fragment Length Polymorphism (AFLP), have been con rmed to be informative in studies on genetic diversity and activities related to the conservation of genetic resources (Bornet and Branchard 2001;Campbell et al 2011;Wang et al 2013;Rahmani et al 2015;Al-Qurainy et al 2018;Rodrigues et al 2020). Among these molecular markers, ISSR was emphasized because it has good reproducibility and does not require prior knowledge of the primer sequence nor high amounts of DNA (Kumar et al 2009;Saoza et al 2015).…”