Micropropagation and production of camptothecin form in vitro plants of Ophiorrhiza mungos

Namdeo, Ajay G.; Priya, Tanu; Bhosale, B.B.

doi:10.1016/s2221-1691(12)60292-5

Cited by 29 publications

(11 citation statements)

References 4 publications

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“…High concentration (> 2.5 mg/L) of NAA, IAA and IBA produced simultaneous rooting and callusing at the thick end root base. Similar observation has been reported in Ophiorrhiza mungos (Namdeo et al, 2012). The callus was highly friable, it could be easily removed from shoots before transferring to field.…”

Section: In Vitro Rooting Of Shootssupporting

confidence: 86%

“…The 90% seeds germination was noted after 7 days on sterile moistened cotton. A high percentage of seed germination (90%) has been reported on sterile moistened filter paper or cotton in Petri dishes or test tubes in various crop species, including snake gourd (Pillai et al, 2008;Devendra et al, 2008 and; Brassica oleracea (Finch-Savage et al, 2006) and Triticum aestivum (Lehner et al, 2006) Ophiorrhiza mungos (Namdeo et al, 2012), Ipomoea sepiaria (Cheruvathur et al, 2015). In the present study, the higher regeneration efficiency was observed in leaf explants excised from 7-10 day-old seedlings (Gogoi et al, 2017), whereas in earlier reports cotyledon explants used were older than 3-4 days (Prem Kumar et al, 2015).…”

Section: In Vitro Seedlingsmentioning

confidence: 99%

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In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

Muthuvel¹,

Vigneswaran²,

Jayabalan³

2017

Int. J. Curr. Res. Biosci. Plant Biol.

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Section: In Vitro Rooting Of Shootssupporting

confidence: 86%

Section: In Vitro Seedlingsmentioning

confidence: 99%

In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

Muthuvel¹,

Vigneswaran²,

Jayabalan³

2017

Int. J. Curr. Res. Biosci. Plant Biol.

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“…Though reports on CPT production through in vitro cultures are available (5,35), the yield was 100 -to 1000 -fold lower than from the field grown plants. Induction of multiple shoots and regeneration of plants have been reported in many species of Ophiorrhiza such as O. mungos (36), O. pumila (14) and O. decumbens (37). The study of Namdeo et al in 2012 (36) in O. mungos exhibited a higher level of CPT in in vitro plants (0.0768 % w/w) than in adventitious buds (0.0026 % w/w).…”

Section: Issn: 2348-1900mentioning

confidence: 96%

Untitled

2018

Plant Sci. Today

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Camptothecin (CPT), a cytotoxic quinoline indole alkaloid, is an anticancer compound. Its two major semi synthetic derivatives, topotecan and irinotecan, are Food and Drug Administration (FDA) approved drugs effectively used for treating different cancer types and sold under the trade names Hycamtin and Camptosar. Among the Ophiorrhiza species, Ophiorrhiza mungos contains the highest CPT level (0.02 % g dw). CPT level was determined in plants before flowering (0.074 ± 0.003 % g dw) and at flowering (0.052 ± 0.002 % g dw). Multiple shoot cultures were induced on seedling-derived explants of O. mungos in half strength MS solid media supplemented with 1.0 mg/L BAP to obtain 12.00 ± 1.22 shoots in 20 days. Repeated subcultures at 20 days interval yielded 20.00 ± 3.71 shoots/subculture of shoot clusters. After elongation, rooting and transplanting, the growth of shoot clusters were studied in both in vitro and same aged naturally grown seedlings. Highest biomass (4.62 ± 0.158 g fw) was obtained in in vitro-derived shoot clusters. CPT increased according to biomass and the maximum CPT was recorded in in vitro rooted shoot clusters after 15 days (0.031 ± 0.001 % g dw). Hence, in vitro-derived rooted shoot clusters of O. mungos cultivated in net-pots for 60 days under shade net house conditions found to be a sustainable source for CPT.

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“…Mhaveer et al (2014) reported that the MS basal medium supplemented with 6-BA (4.0 ppm) + IAA (0.5 ppm) was found to be best for the development of shoot in Acorus calamus Linn. Namdeo et al (2012) consummate a maximum mean of 25 shoots per explants of O. mungos grown on MS medium fortified with Picloram + Thidiazoran + Gibberilicacid (1:2:1) after 60 days of incubation.…”

Section: Establishment Of Multiple Shoots By Pgrsmentioning

confidence: 99%

Original article Elicitors act as a signal transducer in the enhancement of camptothecine production from in vitro cultures of Ophiorrhizamungos L

Nagesha¹,

Ugraiah²,

Ravikanth³

et al. 2018

Ann. Phytomed

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Ophiorrhiza mungos L. is a member of Rubiaceae family, can be a gold standard for the in vitro production of high-value antica ncer compound, camptothecine (CPT) to meet the increasing market demand for medicine. The axillary bud and leaf explants were inoculated on Murashige and Skoog (MS) medium, fortified with different concentrations and combinations of plant growth regulators (PGRs) like N 6 -benzyladenine (BA), linetin (Kn) and indole-3 acetic acid (IAA). The best morphogenic response was observed on MS medium fortified with 8.87 µM l -1 BA, 4.65 µM l -1 Kn and 2.69 µM l -1 IAA, which exhibited the hundred percent regeneration frequency and maximum number of shoots from nodal and leaf explants (63.56 ± 0.28 and 45.03 ± 0.38, respectively) within four weeks. Fortification of 0.87 µM l -1 of gibberellic acid (GA 3 ) along with 4.44 µM l -1 BA enhanced shoots elongation by 7.06 ± 0.19 in 100% of shoot cluster cultures within four weeks. A high percent frequency of rooting (75%) was achieved within 15 days of shoot implantation on half strength MS medium fortified with 4.9 µM l -1 , indole-3-butyric acid (IBA). The rooted plantlets were successfully acclimatized with 100% survival rate in a growth chamber at 25°C, 60 % relative humidity, with 16 h photoperiod. The content of CPT (0.23% w/w) was significantly increased in regenerated plants treated with elicitors compare to the naturally grown plants. The present results evidently showed comparable chemical profile, suggesting that in vitro regenerated plants could be an alternative approach to obtain CPT. These in vitro regenerated flowering plants of O. mungos will be used by floral dip method for over expression of key genes involved in regulating terpenoidindole alkaloid (CPT) biosynthesis.

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Micropropagation and production of camptothecin form in vitro plants of Ophiorrhiza mungos

Cited by 29 publications

References 4 publications

In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

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Original article Elicitors act as a signal transducer in the enhancement of camptothecine production from in vitro cultures of Ophiorrhizamungos L

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