Micropropagation and genetic fidelity analysis in Valeriana jatamansi Jones

Purohit, Sumit; Rawat, Vivek; Jugran, Arun K.; Singh, Rahul Vikram; Bhatt, Indra D.; Nandi, Shyamal K.

doi:10.1016/j.jarmap.2015.01.001

Cited by 18 publications

(12 citation statements)

References 22 publications

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“…1.A). The same challenge was overcome by hormone supplemented MS media in tissue culture as reported by Singh et al (2015) and Purohit et al (2015). AS without supplementation showed 19.44 ± 7.98% new plant emergence (data not shown) from aerial two nodes.…”

Section: Emergence From Nodal Explantssupporting

confidence: 71%

Optimization of aerial node mediated emergence and field performance in lower altitude of a higher altitude specific endangered medicinal plant, Valeriana jatamansi Jones

Sharma¹,

Mondal²

2022

IJGPB

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A study was undertaken to develop a convenient, low cost, and an efficient technique for rapid propagation from aerial node of Valenaria jatamansi Jones directly in artificial soil formulations. The performance of aerial node mediated emergence was also evaluated in higher altitude (HA) as well as lower altitude (LA) to monitor the above and below ground growth parameters in a year in addition to active marker component analysis. The combination of supplemented artificial soil (SAS) having NPK, PSB and Tricoderma asperellum along with critical environment that initiated the high emergence as well as growth benefit at early time point (49 days). The two-node containing aerial cutting (wt.=0.580 ± 0.156g; emergence=87.383 ± 2.483%) showed maximum emergence as compared to one node containing aerial cutting (avg.=0.165 ± 0.016g; avg. emergence=63.883 ± 2.77). Concomitantly, injection of 2 μL of 6-Benzylaminopurine (6-BA; 0.25 mg.lt-1) into an aerial node also enhanced early time point growth benefits like significantly higher number of root initiation. The 4-month-old plant produced around 10 additional aerial nodes that could be explored for next round planting material production and original plant will be directly transplanted into main field with more than 90% survival rate without additional acclimatization and hardening steps. Therefore, a continuous and exponential rate of mass clonal propagation could be achieved from one-time requirement of aerial nodes. The 235 days exposure in lower altitude environment produced a significantly higher number of leaves, larger petiole length, smaller leaf length and breadth as compared to natural habitat grown plant in higher altitude. The above ground mass and root production were significantly higher in LA environment as compared to HA environment, but not in total rhizome production with variation in marker active component. The present study developed for the first time a new plant emergence from aerial node in SAS. Hence, it was breakthrough that aerial node produced Quality Planting Material (QPM) directly. Hence, it will be easy to bypass the costly process like tissue culture for clonal propagation of V. jatamansi, an endangered medicinal plant.

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Section: Emergence From Nodal Explantssupporting

confidence: 71%

Optimization of aerial node mediated emergence and field performance in lower altitude of a higher altitude specific endangered medicinal plant, Valeriana jatamansi Jones

Sharma¹,

Mondal²

2022

IJGPB

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“…Micropropagation has been extensively used for the recovery as well as conservation of endangered species (Nandi et al, 2002;Chandra et al, 2006;Purohit et al, 2015). Few micropropagation protocol have been reported for G. fragrantissima (Table 4), (Bantawa et al, 2011a;Ranyaphi et al, 2011Ranyaphi et al, , 2012 but not in other species of this genus.…”

Section: Micropropagationmentioning

confidence: 99%

Biological and phylogenetic advancements of Gaultheria fragrantissima: Economically important oil bearing medicinal plant

Mukhopadhyay

Bantawa

Mondal

et al. 2016

Industrial Crops and Products

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“…Various techniques have been studied for the propagation of V. jatamansi . For instance, micro-propagation in V. jatamansi took 5 months to develop a plant from culture initiation ( Purohit et al, 2015 ). In Valeriana officinalis (a related species of V. jatamansi ), cultured leaf explants were used for plant regeneration through direct shoot organogenesis ( Abdi and Khui, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Clonal Propagation of Valeriana jatamansi Retains the Essential Oil Profile of Mother Plants: An Approach Toward Generating Homogenous Grade of Essential Oil for Industrial Use

Gautam

Kumar

et al. 2021

Front. Plant Sci.

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Valeriana jatamansi Jones (Syn. V. wallichii DC.) is an aromatic, medicinal herb used as a tranquilizer and in treating sleep disorders. Rhizome is mainly used to extract essential oil (EO) and valepotriates. High quality and economic yield of rhizomes are available in the third year of growth. Therefore, the cultivation of V. jatamansi is not picking up, and over-exploitation of this plant from wild habitats to meet the increasing demand of the pharmaceutical industry is the cause of threat to the genetic diversity of the species. Further, collections from the wild are heterogeneous, resulting in variable produce. The development of clonal lines can ensure uniform quality and yield of rhizome biomass. An effective clonal propagation method was standardized using different hormonal concentrations of naphthalene acetic acid (NAA) on apical shoot cuttings from the selected clone CSIR-IHBT-VJ-05 for different time durations and raised over various planting media. NAA treatment of 50 ppm concentration for 30 min was found optimum for root induction in apical shoots of V. jatamansi. Variations for EO composition within the clone were non-significant, while samples of the control population were variable. The best quality EO (patchouli alcohol ∼62%) was available during the third year of plant growth. A propagation technique for large-scale quality plant material (QPM) production has been standardized to reduce the stress over natural resources and promote V. jatamansi for use in the aromatic and pharmaceutical industry.

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Micropropagation and genetic fidelity analysis in Valeriana jatamansi Jones

Cited by 18 publications

References 22 publications

Optimization of aerial node mediated emergence and field performance in lower altitude of a higher altitude specific endangered medicinal plant, Valeriana jatamansi Jones

Optimization of aerial node mediated emergence and field performance in lower altitude of a higher altitude specific endangered medicinal plant, Valeriana jatamansi Jones

Biological and phylogenetic advancements of Gaultheria fragrantissima: Economically important oil bearing medicinal plant

Clonal Propagation of Valeriana jatamansi Retains the Essential Oil Profile of Mother Plants: An Approach Toward Generating Homogenous Grade of Essential Oil for Industrial Use

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