Microprocessor-controlled, scanning dye laser for spectrometric analytical systems

Perry, James Arthur; Bryant, Melton F.; Malmstadt, H. V.

doi:10.1021/ac50020a019

Cited by 14 publications

(3 citation statements)

References 13 publications

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“…17,18 The introduction of chip-sized devices did not just reduce the overall size of a computational device but also facilitated their mass production and widespread use. Eventually, microprocessors were implemented in chemical laboratory instruments, such as differential titrator, 19 spectrofluorometer, 20 potentiostat, 21 potentiometric detection system, 22,23 polarograph, 24,25 liquid chromatograph, 26 chemiluminescence detector, 27 digital analytical balance, 28,29 and function generator. 30,31 The implementation of microprocessors for controlling the instrument and/or data acquisition 32−36 was indeed a breakthrough that improved the instrumentation for chemistry research and also enhanced the quality of experimental data.…”

Section: Introductionmentioning

confidence: 99%

“…The development of integrated circuits (ICs) sowed the seeds to the birth of chip-sized microprocessors and microcontrollers in the late 1960s and early 1970s. , The introduction of chip-sized devices did not just reduce the overall size of a computational device but also facilitated their mass production and widespread use. Eventually, microprocessors were implemented in chemical laboratory instruments, such as differential titrator, spectrofluorometer, potentiostat, potentiometric detection system, , polarograph, , liquid chromatograph, chemiluminescence detector, digital analytical balance, , and function generator. , The implementation of microprocessors for controlling the instrument and/or data acquisition − was indeed a breakthrough that improved the instrumentation for chemistry research and also enhanced the quality of experimental data. Moreover, some instruments, such as atomic emission spectrometer, mass spectrometer, and potentiometric detector for flow injection system, were equipped with microcomputers for their control and/or data acquisition.…”

Section: Introductionmentioning

confidence: 99%

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Elevating Chemistry Research with a Modern Electronics Toolkit

Prabhu

Urban

2020

Chem. Rev.

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With the rapid development of high technology, chemical science is not as it used to be a century ago. Many chemists acquire and utilize skills that are well beyond the traditional definition of chemistry. The digital age has transformed chemistry laboratories. One aspect of this transformation is the progressing implementation of electronics and computer science in chemistry research. In the past decade, numerous chemistry-oriented studies have benefited from the implementation of electronic modules, including microcontroller boards (MCBs), single-board computers (SBCs), professional grade control and data acquisition systems, as well as field-programmable gate arrays (FPGAs). In particular, MCBs and SBCs provide good value for money. The application areas for electronic modules in chemistry research include construction of simple detection systems based on spectrophotometry and spectrofluorometry principles, customizing laboratory devices for automation of common laboratory practices, control of reaction systems (batch- and flow-based), extraction systems, chromatographic and electrophoretic systems, microfluidic systems (classical and nonclassical), custom-built polymerase chain reaction devices, gas-phase analyte detection systems, chemical robots and drones, construction of FPGA-based imaging systems, and the Internet-of-Chemical-Things. The technology is easy to handle, and many chemists have managed to train themselves in its implementation. The only major obstacle in its implementation is probably one’s imagination.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Elevating Chemistry Research with a Modern Electronics Toolkit

Prabhu

Urban

2020

Chem. Rev.

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“…The fluorometer constructed for TIRF measurements at a liquid/liquid interface was composed of a source of tunable and collimated excitation light, a sample compartment, and the associated detectors and electronics required to measure the interfacial fluorescence. Light pulses of 1 0-ns width and 130-kW peak power were generated by a nitrogen laser constructed in a manner similar to that described by Bryant (32,33). The output of the nitrogen laser was used to pump a DL-14 tunable dye laser (Molectron Corp., Sunnyvale, CA).…”

Section: Fluorometer For Tir-excited Fluorescence Measurementsmentioning

confidence: 99%

Biological membrane modeling with a liquid/liquid interface. Probing mobility and environment with total internal reflection excited fluorescence

Morrison¹,

Weber²

1987

Biophysical Journal

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Total internal reflection of exciting light, in combination with fluorescence intensity and polarization measurements, was used to selectively study fluorescent compounds adsorbed to the interface region between two immiscible liquids. A fluorometer was constructed which provided excitation at variable angles of incidence and allowed sensitive detection of polarized fluorescence emitted from the interface. The compound 4,4'-bis-1-phenylamino-8-naphthalenesulfonate (bis-ANS) was examined at a decalin/water interface and was found to possess remarkable affinity for the interface region with the bulk of the adsorbed molecule residing in the decalin phase. The adsorbed fluorophore displayed an apparent hindered rotation in the plane of the interface with a rotational diffusion coefficient 3- to 12-fold lower than that expected for bis-ANS in solution. While other dyes examined were not found to be significantly surface active, the addition of cationic surfactant sufficed to induce adsorption of the anionic fluorophore 1-aminonaphthalene-3,6,8-trisulfonic acid. This fluoropore was found to reside in an aqueous environment when bound to the interface, and it also exhibited hindered rotation in the plane of the interface. As the concentrations of the dyes were increased, both adsorbed dyes exhibited polarization reductions consistent with excitation energy transfer. Adsorption of bis-ANS was reversed by addition of bovine serum albumin. The membrane protein cytochrome b5 was found not to bind at the decalin/water interface, indicating that interaction with lipid is required for its adherence to biological membranes.

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Fluorescence Detection in Liquid and Gas Chromatography

Froehlich

Wehry

1981

Modern Fluorescence Spectroscopy

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Microprocessor-controlled, scanning dye laser for spectrometric analytical systems

Cited by 14 publications

References 13 publications

Elevating Chemistry Research with a Modern Electronics Toolkit

Elevating Chemistry Research with a Modern Electronics Toolkit

Biological membrane modeling with a liquid/liquid interface. Probing mobility and environment with total internal reflection excited fluorescence

Fluorescence Detection in Liquid and Gas Chromatography

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