Microprobe measurement of Na, K and Cl concentration profiles in epithelial cells and intercellular spaces of rabbit ileum

Gupta, Brij L.; Hall, T. A.; Naftalin, Richard J

doi:10.1038/272070a0

Cited by 107 publications

(42 citation statements)

References 20 publications

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“…M6CI and 77Br tracer fluxes of Cl-determined simultaneously across rabbit ileum in the short-circuit condition. *Note tissue Cl-is consistent with the estimated concentration of Cl-within rabbit ileum using X-ray microprobe microanalysis (Gupta et al 1978) Using 36Cl or 82Br distribution as tracers for Cl-in tissue water gave no significant difference (Table 1).…”

Section: Flux Measurements and Voltage Clampsupporting

confidence: 65%

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The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.

Naftalin¹,

Simmons²

1979

The Journal of Physiology

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SUMMARY1. Theophylline (10 mM) and choleragen change the direction of net Cl-movements across rabbit ileum, in the short-circuit current condition, from absorption to secretion. The specific activity ratio R of C1-tracers within the tissue coming from mucosal and serosal solutions respectively is increased, which is consistent with an increase in Cl-exchange flux across the mucosal border.2. Net Na+ movement is also changed from net absorption to secretion by theophylline and choleragen; the specific activity ratio R of Na+ tracers is raised by theophylline. Because of the large paracellular component to transepithelial Na+ movements, an increase in Na+ exchange flux across the mucosal border is not detected.3. 2,4,6-Triaminopyrimidine (20 mM) which has been previously shown to block paracellular Na+ movements, blocks both the theophylline and choleragen-dependent reversal of net Na+ movement by preventing the decrease in m-s Na flux. The theophylline-dependent increase in the ratio R of Na+ is still present, and is consistent with an increase in Na+ exchange flux across the mucosal border -unmasked by removal of the paracellular flux components. 4. Ouabain (0.1 mM) abolishes net absorption of Na+ and Cl-in control and net secretion of Na+ and Cl-in theophylline-treated tissue. Ouabain does not affect the theophylline-dependent increase in Cl-exchange across the mucosal border.5. Replacement of Ringer Cl-with SO'-or Na+ by choline prevents the effects of theophylline and choleragen on Na+ and C1-fluxes respectively.6. Ethacrynate (0 1 mM) prevents the theophylline-dependent effects on net Na+ movement. Raising ethacrynate to 0-2 mm abolishes the effects of theophylline on Cl-exchange.An interpretation of these results is that theophylline and choleragen raise the Cl-permeability of the brush border. This increases NaCl leakage from the hypertonic lateral intercellular space into the mucosal solution thereby causing secretion. The selective action of triaminopyrimidine and ethacrynate (0.1 mM) on Na+ flux indicates that Na+ and Cl-move via separate transport pathways across the mucosal border.

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Section: Flux Measurements and Voltage Clampsupporting

confidence: 65%

“…This has been confirmed using X-ray electron microprobe microanalysis of the lateral intercellular spaces (Gupta et al 1978). The hypertonicity of the lateral intercellular space is abolished if the tissue Na+ pump activity is inhibited by ouabain.…”

Section: Discussionmentioning

confidence: 69%

The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.

Naftalin¹,

Simmons²

1979

The Journal of Physiology

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“…In contrast , cultured mammalian cells have not frequently been used for that purpose, although one can easily select pertinent types of cells from different sources and examine the growth phenomena in relation to cation transport. One reason for the obstruction to use of cultured cells is the difficulty of controlling the intracellular concentrations of alkali metal cations, which seems partly to be due to uneven distribution of the cations inside the cells (PALMER and CIVAN, 1977;GUPTA and HALL, 1978). HeLa cells are known to have much denser [3H] ouabain-binding sites on the cell membrane than red cells (BAKER and WILLIS, 1969).…”

Section: Cellsmentioning

confidence: 99%

Interrelation between membrane transport and the contents of alkali metal cations in HeLa cells.

et al. 1982

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“…[6] and X-ray microprobe microanalysis of frozen hydrated sections [7]. Use of the impermeant acid form of SBFI, a fluorescent probe for Na+, along with high resolution video-enhanced microscopy to detect the fluorescent emission of SBFI, is a more sensitive and convenient tool for detecting extracellular Na+ than these other methods [1,2] and its use provides an opportunity to test a new hypothesis relating to colonic absorptive function.…”

Section: Introduction Extracetlularmentioning

confidence: 99%

Video enhanced imaging of the fluorescent Na⁺ probe SBFI indicates that colonic crypts absorb fluid by generating a hypertonic interstitial fluid

Naftalin

Pedley²

1990

FEBS Letters

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Extraeellular a~~uiationof Na+ detected by video-enhanced microscopic imaging of the impermeant fluorescent probe SBFI[1,2] eonfirms the view that colonic crypts produce a hypertonic absorbate ca 1000 mOsm~kg-ts thereby generating a large osmotic pressure across the crypt wall.This creates a high fluid tension within the crypt lumen, sufficient to dehydrate faeces [3,4]. When bathed in Tyrode the SBFI+Na+ fluorescence indicates a [Na+) ca 750 mM within the interstitial space of metabolizing rat descending colon. There is no evidence ofinterstitial Na+ accumulation in octanol (2 mM) or in rabbit colon incubated with 1.0 mM ouabain and no evidence of Nat secretion via the crypt lumen during absorption 151.

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Microprobe measurement of Na, K and Cl concentration profiles in epithelial cells and intercellular spaces of rabbit ileum

Cited by 107 publications

References 20 publications

The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.

The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.

Interrelation between membrane transport and the contents of alkali metal cations in HeLa cells.

Video enhanced imaging of the fluorescent Na⁺ probe SBFI indicates that colonic crypts absorb fluid by generating a hypertonic interstitial fluid

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Microprobe measurement of Na, K and Cl concentration profiles in epithelial cells and intercellular spaces of rabbit ileum

Cited by 107 publications

References 20 publications

The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.

The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum.

Interrelation between membrane transport and the contents of alkali metal cations in HeLa cells.

Video enhanced imaging of the fluorescent Na+ probe SBFI indicates that colonic crypts absorb fluid by generating a hypertonic interstitial fluid

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Video enhanced imaging of the fluorescent Na⁺ probe SBFI indicates that colonic crypts absorb fluid by generating a hypertonic interstitial fluid