Microporous titanosilicates Cu<sup>2+</sup>– and Co<sup>2+</sup>–ETS-4 for storage and slow release of therapeutic nitric oxide

Pinto, Moisés L.; Fernandes, Ana C.; Rocha, João; Ferreira, Artur; Antunes, Fernando; Pires, João

doi:10.1039/c3tb20929f

Cited by 21 publications

(24 citation statements)

References 22 publications

(31 reference statements)

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“…However, as shown below, NO loaded MIP‐177 will produce a positive effect on cell migration at concentrations of 90 μg mL −1 , for which no toxicity was found. Compared with previous work, where all porous materials studied for the same purpose presented at least 20 % toxicity with HeLa cells after 72 hours, no toxicity was observed in the present case with HeLa cells (a comparison with other MOFs is presented in the Supporting Information).…”

Section: Resultscontrasting

confidence: 70%

Tuning Cellular Biological Functions Through the Controlled Release of NO from a Porous Ti‐MOF

et al. 2020

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Materials for the controlled release of nitric oxide (NO) are of interest for therapeutic applications. However, to date, many suffer from toxicity and stability issues, as well as poor performance. Herein, we propose a new NO adsorption/release mechanism through the formation of nitrites on the skeleton of a titanium‐based metal–organic framework (MOF) that we named MIP‐177, featuring a suitable set of properties for such an application: (i) high NO storage capacity (3 μmol mg−1solid), (ii) excellent biocompatibility at therapeutic relevant concentrations (no cytotoxicity at 90 μg mL−1 for wound healing) due to its high stability in biological media (<9 % degradation in 72 hours) and (iii) slow NO release in biological media (≈2 hours for 90 % release). The prospective application of MIP‐177 is demonstrated through NO‐driven control of mitochondrial respiration in cells and stimulation of cell migration, paving the way for the design of new NO delivery systems for wound healing therapy.

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Section: Resultscontrasting

confidence: 70%

Tuning Cellular Biological Functions Through the Controlled Release of NO from a Porous Ti‐MOF

et al. 2020

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“…NO adsorption capability depends on several material characteristics, including the pore and the adsorbed molecule sizes, the cation nature, its distribution and concentration throughout the porous structure, the polarization and the quantity of water [10]. In Figure 1 are depicted the different inorganic porous materials selected for the NO-releasing assessment that had previously shown potential for NO storage [16][17][18][19]. Zeolite A ( Figure 1a) contains alternated SiO 4 and AlO 4 tetrahedra that share corners to produce the open framework.…”

Section: No Adsorption Capacity Of Different Porous Materialsmentioning

confidence: 99%

“…Additionally, modified specimens from ETS-4 and ETS-10 were also studied based on the exchanging extra-framework cations (Na + by Co + ) (Co-ETS-4) and on the isomorphic substitution of silicon by aluminium (ETAS-10), respectively. In the case of Co-ETS-4, the Co 2+ provide possibility to a stronger coordination of NO without promoting its degradation [17]. In addition, the exchange of Na + by Co 2+ increased the pore space available for adsorption, since exchanging a monovalent with a divalent cation leads to a 2:1 exchange ratio.…”

Section: No Adsorption Capacity Of Different Porous Materialsmentioning

confidence: 99%

A Comparison of Different Approaches to Quantify Nitric Oxide Release from NO-Releasing Materials in Relevant Biological Media

et al. 2020

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The development of solid materials that deliver nitric oxide (NO) are of interest for several therapeutic applications. Nevertheless, due to NO’s reactive nature, rapid diffusion and short half-life, reporting their NO delivery characteristics is rather complex. The full knowledge of this parameter is fundamental to discuss the therapeutic utility of these materials, and thus, the NO quantification strategy must be carefully considered according to the NO-releasing scaffold type, to the expected NO-releasing amounts and to the medium of quantification. In this work, we explore and discuss three different ways of quantifying the release of NO in different biological fluids: haemoglobin assay, Griess assay and NO electrochemical detection. For these measurements, different porous materials, namely zeolites and titanosilicates were used as models for NO-releasing platforms. The oxyhaemoglobin assay offers great sensitivity (nanomolar levels), but it is only possible to monitor the NO release while oxyhaemoglobin is not fully converted. On the other hand, Griess assay has low sensitivity in complex biological media, namely in blood, and interferences with media make NO measurements questionable. Nevertheless, this method can measure micromolar amounts of NO and may be useful for an initial screening for long-term release performance. The electrochemical sensor enabled real-time measurements in a variety of biological settings. However, measured NO is critically low in oxygenated and complex media, giving transient signals, which makes long-term quantification impossible. Despite the disadvantages of each method, the combination of all the results provided a more comprehensive NO release profile for these materials, which will help to determine which formulations are most promising for specific therapeutic applications. This study highlights the importance of using appropriate NO quantification tools to provide accurate reports.

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“…10 In the case of the microporous titanosilicate materials, recent studies show that these materials have potential as drug carriers, with low cytotoxicity. [11][12][13] The goal of this work is to study the potential of several silica microporous structures as hosts for 5-uorouracil (5-FU) as drug delivery systems for in vitro models of colorectal and breast cancers. 5-FU is a pyrimidine analog antimetabolite, with a general spectrum of activity against solid tumors, such as breast, colorectal, liver and brain cancer, alone or in combination with other chemotherapeutic agents.…”

Section: Introductionmentioning

confidence: 99%

Comparison of different silica microporous structures as drug delivery systems for in vitro models of solid tumors

et al. 2017

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Microporous titanosilicates Cu²⁺– and Co²⁺–ETS-4 for storage and slow release of therapeutic nitric oxide

Cited by 21 publications

References 22 publications

Tuning Cellular Biological Functions Through the Controlled Release of NO from a Porous Ti‐MOF

Tuning Cellular Biological Functions Through the Controlled Release of NO from a Porous Ti‐MOF

A Comparison of Different Approaches to Quantify Nitric Oxide Release from NO-Releasing Materials in Relevant Biological Media

Comparison of different silica microporous structures as drug delivery systems for in vitro models of solid tumors

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Microporous titanosilicates Cu2+– and Co2+–ETS-4 for storage and slow release of therapeutic nitric oxide

Cited by 21 publications

References 22 publications

Tuning Cellular Biological Functions Through the Controlled Release of NO from a Porous Ti‐MOF

Tuning Cellular Biological Functions Through the Controlled Release of NO from a Porous Ti‐MOF

A Comparison of Different Approaches to Quantify Nitric Oxide Release from NO-Releasing Materials in Relevant Biological Media

Comparison of different silica microporous structures as drug delivery systems for in vitro models of solid tumors

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Microporous titanosilicates Cu²⁺– and Co²⁺–ETS-4 for storage and slow release of therapeutic nitric oxide