Microporous poly(glycidyl methacrylate-<i>co</i>-ethylene glycol dimethyl acrylate) microspheres: synthesis, functionalization and applications

Xiao, Jingyuan; Lu, Qingbiao; Cong, Hailin; Shen, Youqing; Yu, Bing

doi:10.1039/d1py00834j

Cited by 25 publications

(15 citation statements)

References 169 publications

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“…Monosized pGMA microspheres were prepared by the dispersionpolymerization method [25][26][27] with minor modifications. Briefly, 2.4 g PVP K30 was dispersed into 72 g ethanol in a 250-mL three-necked flask with a mechanical stirrer.…”

Section: Fabrication Of Monosized Pgma Microspheresmentioning

confidence: 99%

Characterization of a heptapeptide‐modified microsphere for oriented antibody immobilization

Bai

Zhang

Zhu

et al. 2022

Journal of Peptide Science

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Oriented immobilization of antibodies is important for the effective recognition of target antigens. In this paper, a heptapeptide ligand, HWRGWVC (HC7), was modified onto non-porous monosized poly(glyceryl methacrylate) (pGMA) microspheres (named pGMA-HC7) to explore the antibody immobilization behaviors. Characterization of the microspheres by particle size analyzer, scanning electron microscopy, Fourier transform infrared spectroscopy, and reversed-phase chromatography proved the success of each fabrication step. The capacity and activity of antibody immobilization through HC7 were studied using immunoglobulin G (IgG) as a model antibody and horseradish peroxidase (HRP) as a model antigen. Additionally, IgG immobilizations on pGMA microspheres by nonspecific adsorption and covalent coupling through carbodiimide chemistry were conducted for comparison. pGMA-HC7 exhibited an IgG adsorption capacity of 3-4 mg/g in 10 min by the specific binding of HC7 without nonspecific interactions. Notably, the ligand HC7 showed a by two orders of magnitude stronger affinity for IgG than its original hexapeptide ligand HWRGWV. Moreover, the capacity and activity of the immobilized anti-HRP antibody on pGMA-HC7 were 1.6-fold and 3-fold higher than those of the covalent coupling, respectively. The results proved the superior role of HWRGWVC in the affinity binding of antibody and the potential of pGMA-HC7-25 in immunoassay and immunodiagnostic applications.

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Section: Fabrication Of Monosized Pgma Microspheresmentioning

confidence: 99%

Characterization of a heptapeptide‐modified microsphere for oriented antibody immobilization

Bai

Zhang

Zhu

et al. 2022

Journal of Peptide Science

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“…Among the above techniques, adsorption technology has advantages, such as its ease, high efficacy, insensitivity to the contaminant substance, and flexibility. 19–24 Several adsorbent materials, such as fly ash, 25 agricultural wastes, 26,27 and wood wastes 28 have been developed for the adsorptive removal of MB dye, but are not selective. In comparison, polymer-based adsorbents are inexpensive and easy to synthesize as well as selective in nature.…”

Section: Introductionmentioning

confidence: 99%

Selective dye adsorption by pH modulation on chemically modified nanopolyaniline by N-grafting of maleic acid

Rath,

Mohanty,

Patra

2023

Polym. Chem.

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“…Porous polymer particles have attracted considerable interest in various applications ranging from clinical diagnostics [1], immobilization support for biocatalysts [2], separation processes like chromatography [3][4][5][6], ion exchange phases [7] to applications as adsorption materials [8,9] or as hard-templates [10][11][12] for inorganic materials with defined structures. In particular, porous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (p(GMA-co-EDMA)) has been widely used, as the epoxy ring of GMA can be derivatized in various ways by ring opening reactions [2,[13][14][15]. This allows application-specific functionalization and makes the p(GMA-co-EDMA) a versatile platform polymer.…”

Section: Introductionmentioning

confidence: 99%

Rational Design of Pore Parameters in Monodisperse Porous Poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) Particles Based on Response Surface Methodology

et al. 2022

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Monodisperse porous poly(glycidyl methacrylate-co–ethylene glycol dimethacrylate) particles are widely applied in different fields, as their pore properties can be influenced and functionalization of the epoxy group is versatile. However, the adjustment of parameters which control morphology and pore properties such as pore volume, pore size and specific surface area is scarcely available. In this work, the effects of the process factors monomer:porogen ratio, GMA:EDMA ratio and composition of the porogen mixture on the response variables pore volume, pore size and specific surface area are investigated using a face centered central composite design. Non-linear effects of the process factors and second order interaction effects between them were identified. Despite the complex interplay of the process factors, targeted control of the pore properties was possible. For each response a response surface model was derived with high predictive power (all R2predicted > 0.85). All models were tested by four external validation experiments and their validity and predictive power was demonstrated.

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Microporous poly(glycidyl methacrylate-co-ethylene glycol dimethyl acrylate) microspheres: synthesis, functionalization and applications

Abstract: As a new kind of functional material, micron-sized porous polymer microspheres are a hot research topic in the field of polymer materials. poly (glycidyl methacrylate) microspheres have been widely used...

Cited by 25 publications

References 169 publications

Characterization of a heptapeptide‐modified microsphere for oriented antibody immobilization

Characterization of a heptapeptide‐modified microsphere for oriented antibody immobilization

Selective dye adsorption by pH modulation on chemically modified nanopolyaniline by N-grafting of maleic acid

Rational Design of Pore Parameters in Monodisperse Porous Poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) Particles Based on Response Surface Methodology

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