Microplastics: What happens in the human digestive tract? First evidences in adults using in vitro gut models

Fournier, E; Lévêque, Mathilde; Ruiz, Philippe; Ratel, Jérémy; Durif, Claude; Chalancon, Sandrine; Amiard, Frederic; Edely, Mathieu; Bézirard, Valérie; Gaultier, Eric; Lamas, Bruno; Houdeau, Eric; Lagarde, Fabienne; Engel, Erwan; Etienne‐Mesmin, Lucie; Blanquet‐Diot, Stéphanie; Mercier‐Bonin, Muriel

doi:10.1016/j.jhazmat.2022.130010

Cited by 49 publications

(22 citation statements)

References 102 publications

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“…polymer type, size, shape). In our previous study of the adult microbiota, no change in bacterial diversity was observed after exposure to PE MPs, suggesting that the immature infant gut microbiota may be more sensitive to MPs than the mature microbiota [44]. When mice were exposed to PE MPs, the microbial shifts observed in fecal samples were study-dependent and not comparable to the changes observed in Tm-ARCOL [72,76], certainly due to the large discrepancies between human and rodent digestion and microbiome [45].…”

Section: Discussionmentioning

confidence: 78%

“…In this context, as an extension of a previous study by us on adult microbiota [44], we investigated for the first time the impact of oral exposure to PE MPs on the infant microbiome and the intestinal epithelium/mucus barrier. Due to major ethical constraints linked to clinical studies on young populations, but also the striking differences between human and rodent digestive physiology -including microbiota- [45], these experiments were carried out using an innovative in vitro approach, combining the Toddler mucosal Artificial Colon (Tm-ARCOL, This preprint research paper has not been peer reviewed.…”

Section: Mps Injected Intraperitoneally Into Pregnant Mice Resulted I...mentioning

confidence: 99%

“…Electronic copy available at: https://ssrn.com/abstract=4229664 P r e p r i n t n o t p e e r r e v i e w e d polyethylene terephthalate -PET-and not PE, also of different size and shape (160 ± 110 µm, irregular morphology) [66]. We also recently performed a study in the Mucosal Artificial Colon (M-ARCOL) where we assessed the impact of repeated exposure to PE MPs on the composition and activity of the adult gut microbiota [44]. Here, as an extension of this work and in order to be as close as possible to the human situation, we have chosen to evaluate in Tm-ARCOL the effect of a physiological dose of MPs at 21 mg/day according to recent exposure data [67].…”

Section: Discussionmentioning

confidence: 99%

“…DSC and TGA analysis of PE MPs showed the purity of the PE powder and characterized it as low-density PE. PE MP microscopy count indicated a size between 1 and 2 µm for 50% of 156 beads analysed [44]. Scanning electron microscopy of samples collected in the luminal phase and mucosal beads in Tm-ARCOL (Fig.…”

Section: Characteristics Of Pe Mps and Interactions With The Infant G...mentioning

confidence: 96%

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Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

Fournier

Ratel

Denis

et al. 2023

Journal of Hazardous Materials

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Section: Discussionmentioning

confidence: 78%

Section: Mps Injected Intraperitoneally Into Pregnant Mice Resulted I...mentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Characteristics Of Pe Mps and Interactions With The Infant G...mentioning

confidence: 96%

See 2 more Smart Citations

Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

Fournier

Ratel

Denis

et al. 2023

Journal of Hazardous Materials

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“…(2020);Fournier et al (2023) Caco-2+Raji-B (M-cell model) Enterocytes and microfold cells Schimpel et al (2014) Cell lines, triple culture Caco-2+HT29-MTX-E12+THP-1 Enterocytes, goblet cells and macrophages Busch et al (2021a); Busch et al (2021b) Caco-2+HT29+Raji-B (M-cell model) Enterocytes, goblet cells and microfold cells Mahler et al (2012); Schimpel et al (2014); Walczak et al (2015c); Domenech et al (2020) monocyte-derived dendritic cells. b MDM: monocyte-derived macrophages.…”

mentioning

confidence: 99%

Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models

et al. 2023

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Plastic particles in the nanometer range–called nanoplastics–are environmental contaminants with growing public health concern. As plastic particles are present in water, soil, air and food, human exposure via intestine and lung is unavoidable, but possible health effects are still to be elucidated. To better understand the Mode of Action of plastic particles, it is key to use experimental models that best reflect human physiology. Novel assessment methods like advanced cell models and several alternative approaches are currently used and developed in the scientific community. So far, the use of cancer cell line-based models is the standard approach regarding in vitro nanotoxicology. However, among the many advantages of the use of cancer cell lines, there are also disadvantages that might favor other approaches. In this review, we compare cell line-based models with stem cell-based in vitro models of the human intestine and lung. In the context of nanoplastics research, we highlight the advantages that come with the use of stem cells. Further, the specific challenges of testing nanoplastics in vitro are discussed. Although the use of stem cell-based models can be demanding, we conclude that, depending on the research question, stem cells in combination with advanced exposure strategies might be a more suitable approach than cancer cell lines when it comes to toxicological investigation of nanoplastics.

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Fighting microplastics: The role of dietary fibers in protecting health

Wang,

Zhang

et al. 2024

Food Frontiers

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Microplastics (MPs) have emerged as a significant food‐related risk factor, posing potential threats to human health through dietary intake and the food chain. This review comprehensively analyses the impact of MPs as a novel food safety risk factor on human health (in particular on the gastrointestinal route). Furthermore, we explore the potential mechanisms by which dietary fibers (DFs) may alleviate the health risks associated with MPs. The impact of DFs on human health is intricately linked to factors such as their size, concentration, and composition. We characterize current knowledge and highlight gaps. Although DFs may be a potential strategy to reduce the impact of MPs on organism health, more in‐depth studies are needed to determine their practical effects and application prospects. In particular, research on MPs that actively reduce intake in vivo remains relatively limited and needs to receive more attention from the scientific community.

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Microplastics: What happens in the human digestive tract? First evidences in adults using in vitro gut models

Cited by 49 publications

References 102 publications

Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models

Fighting microplastics: The role of dietary fibers in protecting health

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