Microplastics impair digestive performance but show little effects on antioxidant activity in mussels under low pH conditions

Wang, Xinghuo; Huang, Wei; Wei, Shuaishuai; Shang, Yingxin; Gu, Huaxin; Wu, Fangzhu; Lan, Zhaohui; Hu, Menghong; Shi, Huahong; Wang, Youji

doi:10.1016/j.envpol.2019.113691

Cited by 106 publications

(28 citation statements)

References 66 publications

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“…following a 7-day exposure to PS-MP, and Magara et al (2018) showed no effect on SOD activity in the gills or digestive glands of M. edulis exposed to 100-1000 PE-MP mL -1 for 4 days. Wang et al (2020) identified a non-significant increase in SOD activity in the digestive glands of the mussel Mytilus coruscus following 24 h exposure to microplastics, and no changes in SOD activity with longer exposure periods. Thiobarbituric acid reactive substances (TBARS) form as a byproduct of lipid peroxidation, stemming from the oxidative degradation of lipids by reactive oxygen species.…”

Section: Oxidative Balance: Sod and Tbarsmentioning

confidence: 88%

Microplastics, microfibres and nanoplastics cause variable sub-lethal responses in mussels (Mytilus spp.)

Cole

Liddle

Consolandi

et al. 2020

Marine Pollution Bulletin

141

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We compare the toxicity of microplastics, microfibres and nanoplastics on mussels. Mussels (Mytilus spp.) were exposed to 500 ng mL-1 of 20 µm polystyrene microplastics, 10x30 µm polyamide microfibres or 50 nm polystyrene nanoplastics for 24 h or 7 days. Biomarkers of immune response, oxidative stress response, lysosomal destabilisation and genotoxic damage were measured in haemolymph, digestive gland and gills. Microplastics and microfibres were observed in the digestive glands, with significantly higher plastic concentrations after 7-days exposure (ANOVA, P<0.05). Nanoplastics had a significant effect on hyalinocyte-granulocyte ratios (ANOVA, P<0.05), indicative of a heightened immune response. SOD activity was significantly increased followed 24 h exposure to plastics (two-way ANOVA, P<0.05), but returned to normal levels after 7-days exposure. No evidence of lysosomal destabilisation or genotoxic damage was observed from any form of plastic. The study highlights how particle size is a key factor in plastic particulate toxicity.

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Section: Oxidative Balance: Sod and Tbarsmentioning

confidence: 88%

Microplastics, microfibres and nanoplastics cause variable sub-lethal responses in mussels (Mytilus spp.)

Cole

Liddle

Consolandi

et al. 2020

Marine Pollution Bulletin

141

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show abstract

“…In terms of suitable species, controlled exposure studies specifically examining ingestion and depuration kinetics rarely consider organisms likely exposed to and contaminated with microplastics in the marine environment. For example, ingestion and depuration studies have mainly focused on aquatic invertebrates (Wang X. et al, 2019;Chae and An, 2020;Ehlers et al, 2020), and freshwater fish (Grigorakis et al, 2017;Xiong et al, 2019;Hoang and Felix-Kim, 2020). In contrast, only four ingestion and depuration studies have been conducted on brackish/marine fish (Manabe et al, 2011;Cong et al, 2019;Bour et al, 2020b) despite these being some of the most frequently reported organisms contaminated with microplastics (Lusher, 2015;Kroon F. J. et al, 2018;Jensen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Ingestion and Depuration of Microplastics by a Planktivorous Coral Reef Fish, Pomacentrus amboinensis

Santana

Dawson

Motti

et al. 2021

Front. Environ. Sci.

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Microplastics are ubiquitous contaminants in marine environments and organisms. Concerns about potential impacts on marine organisms are usually associated with uptake of microplastics, especially via ingestion. This study used environmentally relevant exposure conditions to investigate microplastic ingestion and depuration kinetics of the planktivorous damselfish, Pomacentrus amboinensis. Irregular shaped blue polypropylene (PP) particles (longest length 125–250 μm), and regular shaped blue polyester (PET) fibers (length 600–700 μm) were selected based on physical and chemical characteristics of microplastics commonly reported in the marine environment, including in coral reef ecosystems. Individual adult damselfish were exposed to a single dose of PP particles and PET fibers at concentrations reported for waters of the Great Barrier Reef (i.e., environmentally relevant concentrations, ERC), or future projected higher concentrations (10x ERC, 100x ERC). Measured microplastic concentrations were similar to their nominal values, confirming that PP particles and PET fibers were present at the desired concentrations and available for ingestion by individual damselfish. Throughout the 128-h depuration period, the 88 experimental fish were sampled 2, 4, 8, 16, 32, 64, and 128-h post microplastic exposure and their gastrointestinal tracts (GIT) analyzed for ingested microplastics. While damselfish ingested both experimental microplastics at all concentrations, body burden, and depuration rates of PET fibers were significantly larger and longer, respectively, compared to PP particles. For both microplastic types, exposure to higher concentrations led to an increase in body burden and lower depuration rates. These findings confirm ingestion of PP particles and PET fibers by P. amboinensis and demonstrate for the first time the influence of microplastic characteristics and concentrations on body burden and depuration rates. Finally, despite measures put in place to prevent contamination, extraneous microplastics were recovered from experimental fish, highlighting the challenge to completely eliminate contamination in microplastic exposure studies. These results are critical to inform and continuously improve protocols for future microplastics research, and to elucidate patterns of microplastic contamination and associated risks in marine organisms.

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Section: Discussionmentioning

confidence: 99%

“…Yet, the mechanisms of this reduction in activity are unknown. To this end, Wang et al (2020) [35] showed a decrease in digestive gland amylase activity in a small sample size of hard-shelled mussel M. coruscus, exposed to > 10,000 particles/L of 2 μm-sized polystyrene spheres. Hard shelled mussels (M. coruscus and M. californianus) and blue mussels (Mytilus edulis complex), including M. galloprovincialis are spatially segregated in nature.…”

Section: Plos Onementioning

confidence: 99%

“…Importantly, an examination of a range of enzymes including other carbohydrases, lipases, and proteases is necessary to fully understand impacts on digestive gland function in M. galloprovincialis. In this context Wang (2020) observed negative effects on protease and lipase activity in M. coruscus [35]. Finally, studies of microplastic effects on gill and digestive gland should be conducted in series so that mechanisms of the effects can ultimately be revealed.…”

Section: Plos Onementioning

confidence: 99%

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The observation of starch digestion in blue mussel Mytilus galloprovincialis exposed to microplastic particles under varied food conditions

et al. 2021

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Microplastic continues to be an environmental concern, especially for filter feeding bivalves known to ingest these particles. It is important to understand the effects of microplastic particles on the physiological performance of these bivalves and many studies have investigated their impact on various physiological processes. This study investigated the effects of microplastic (10 μm) on digestive enzyme (amylase) activity of Mytilus galloprovincialis at 55,000 and 110,000 microplastic particles/L under laboratory conditions. Additionally, our study measured the expression of an isoform of Hsp70 in the gills to assess whether or not these particles may cause protein denaturation. Results revealed that this regime negatively affect the ability of M. galloprovincialis to digest starch under high food conditions but not low food conditions. Exposure to extreme levels of microplastic raised amylase activity. Furthermore, Hsp70 transcript abundance was not elevated in treatment mussels. These results show that mussels may be resilient to current microplastic pollution levels in nature.

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Microplastics impair digestive performance but show little effects on antioxidant activity in mussels under low pH conditions

Cited by 106 publications

References 66 publications

Microplastics, microfibres and nanoplastics cause variable sub-lethal responses in mussels (Mytilus spp.)

Microplastics, microfibres and nanoplastics cause variable sub-lethal responses in mussels (Mytilus spp.)

Ingestion and Depuration of Microplastics by a Planktivorous Coral Reef Fish, Pomacentrus amboinensis

The observation of starch digestion in blue mussel Mytilus galloprovincialis exposed to microplastic particles under varied food conditions

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