Microplastics altered cellular responses, physiology, behaviour, and regeneration of planarians feeding on contaminated prey

Silva, Sara A.M.; Prata, Joana C.; Dias‐Pereira, Patrícia; Rodrigues, Andreia C.M.; Soares, Amadeu M.V.M.; Sarmento, Renato Almeida; Rocha-Santos, Teresa; Gravato, Carlos; Silva, Ana L. Patrício

doi:10.1016/j.scitotenv.2023.162556

Cited by 5 publications

(9 citation statements)

References 51 publications

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“…Post-exposure alterations in locomotor activity have also been utilised as indicators of stress induced by ammonia in the planarian Polycelis felina [33]. The potential impact of PU particles and microplastic particles in general on the locomotion of planarians has been previously reported, and results in this study correspond with the absence of PU effect on locomotor activity shown in [28]. However, alterations in locomotor activity have been documented in organisms exposed to microplastics or prey contaminated with microplastics.…”

Section: Resultssupporting

confidence: 72%

“…ETS has been evaluated in planarians, suggesting that energy allocation refers to the prioritisation of energy resources within a planarian to support homeostasis. Changes in energy allocation patterns can occur in response to environmental stressors or challenges, and these changes may have implications for overall health and fitness [28,51,52]. LDH facilitates the conversion of pyruvate to lactate within the anaerobic pathway of cellular energy generation [53].…”

Section: Resultsmentioning

confidence: 99%

“…Lipid peroxidation (LPO) concentrations were assessed by measuring thiobarbituric acid reactive substances (TBARS) following the protocols established by Ohkawa et al [27] and described in detail in Silva et al [28]. The reaction mixture contained sample, trichloroacetic acid (TCA), 2-thiobarbituric acid (TBA), trizma hydrochloride, Tris-HCl, and diethylene triamine pentaacetic acid (DTPA).…”

Section: Oxidative Stressmentioning

confidence: 99%

“…Specific activity was calculated based on ε = 13.60 × 10 3 M −1 cm −1 . Electron transport system (ETS) activity was measured according to the protocol described in [28]. The following reagents were used: β-NADPH, reduced β-nicotinamide adenine dinucleotide (β-NADH), and p-iodonitrotetrazolium (INT).…”

Section: Metabolic Biomarkersmentioning

confidence: 99%

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A Dangerous Couple: Sequential Effect of Phosphorus Flame-Retardant and Polyurethane Decrease Locomotor Activity in Planarian Girardia tigrina

Bjedov,

Barbosa,

Oliveira

et al. 2024

Biology

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Understanding the interplay among organophosphorus flame retardants (OPFRs), microplastics, and freshwater organisms is crucial for unravelling the dynamics within freshwater environments and foreseeing the potential impacts of organic pollutants and plastic contamination. For that purpose, the present research assessed the exposure impact of 10 mg L−1 flame-retardant aluminium diethylphosphinate (ALPI), 10 μg mg−1liver microplastics polyurethane (PU), and the combination of ALPI and PU on the freshwater planarian Girardia tigrina. The exposure to both ALPI and PU revealed a sequential effect, i.e., a decrease in locomotor activity, while oxidative stress biomarkers (total glutathione, catalase, glutathione S-transferase, lipid peroxidation) and metabolic responses (cholinesterase activity, electron transport system, and lactate dehydrogenase) remained unaffected. Despite this fact, it was possible to observe that the range of physiological responses in exposed organisms varied, in particular in the cases of the electron transport system, cholinesterase activity, glutathione S-transferase, catalase, and levels of total glutathione and proteins, showing that the energetic costs for detoxification and antioxidant capacity might be causing a lesser amount of energy allocated for the planarian activity. By examining the physiological, behavioural, and ecological responses of planarians to these pollutants, insights can be gained into broader ecosystem-level effects and inform strategies for mitigating environmental risks associated with OPFRs and microplastic pollution in freshwater environments.

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Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Oxidative Stressmentioning

confidence: 99%

Section: Metabolic Biomarkersmentioning

confidence: 99%

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A Dangerous Couple: Sequential Effect of Phosphorus Flame-Retardant and Polyurethane Decrease Locomotor Activity in Planarian Girardia tigrina

Bjedov,

Barbosa,

Oliveira

et al. 2024

Biology

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“…The environmental hazard of petroleum-based plastics has been increasingly evaluated for many species (mostly aquatic) [3]. Exposure to it has caused oxidative stress or damage to marine and estuarine bivalves [4,5] and planarias [6], damage to gut epithelium, inflammatory responses in freshwater chironomids [7], and the depletion of energy reserves in marine worms [8]. On the other hand, no effect on the life cycle of chironomids and zooplankton has been shown [9,10], while the successful egestion of ingested microplastics by chironomid and zebrafish has been demonstrated [11].…”

Section: Introductionmentioning

confidence: 99%

Polylactic Acid-Based Microplastic Particles Induced Oxidative Damage in Brain and Gills of Goldfish Carassius auratus

Khosrovyan

Melkonyan

Rshtuni

et al. 2023

Water

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The effect of 96 h exposure of the goldfish Carassius auratus to two different types of bioplastic particles, originating from commercial shopping bag (Bag, ~5 mm) and polylactic acid-based (PLA) cup (Cup, ≤5 mm), and petroleum-based polyamide particles (PA, 0–180 µm) was studied. All particles were studied as virgin and after simulated UV-degradation (at concentration 30 mg L−1). The experiments were conducted according to OECD 203 test guidelines. The toxicity endpoint evaluated in fish brain and gills was lipid peroxidation (LPO) quantified as nmol thiobarbituric acid reactive substances (TBARs) mg−1 protein. The results indicated that indicatively compostable PLA bioplastic Cup induced significant LPO in the brain and/or gills of the goldfish, in contrast to the particles from Bag and PA (in the brain, Cup > Bag > PA; in the gills, Cup > Bag). The UV-degradation of the particles of all studied types had no significant effect on the LPO level compared to virgin particles. While the increase of LPO in fish gills and brain upon exposure to PLA-plastic particles can be transitory in the long-term perspective, our results point to the necessity of a thorough investigation of the hazard of bioplastics at different state of environmental degradation/weathering.

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Impact of diethyl phthalate on freshwater planarian behaviour, regeneration, and antioxidant defence

Verma,

Khare,

Chakraborty

et al. 2024

Aquatic Toxicology

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Microplastics altered cellular responses, physiology, behaviour, and regeneration of planarians feeding on contaminated prey

Cited by 5 publications

References 51 publications

A Dangerous Couple: Sequential Effect of Phosphorus Flame-Retardant and Polyurethane Decrease Locomotor Activity in Planarian Girardia tigrina

A Dangerous Couple: Sequential Effect of Phosphorus Flame-Retardant and Polyurethane Decrease Locomotor Activity in Planarian Girardia tigrina

Polylactic Acid-Based Microplastic Particles Induced Oxidative Damage in Brain and Gills of Goldfish Carassius auratus

Impact of diethyl phthalate on freshwater planarian behaviour, regeneration, and antioxidant defence

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