Microplastics aggravate the adverse effects of BDE-47 on physiological and defense performance in mussels

Gu, Huaxin; Wei, Shuaishuai; Hu, Menghong; Huang, Wei; Wang, Xinghuo; Shang, Yingxin; Li, Liang; Shi, Huahong; Wang, Youji

doi:10.1016/j.jhazmat.2020.122909

Cited by 79 publications

(21 citation statements)

References 64 publications

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“…The release of ROS from hemocytes is considered as a key internal defense mechanism through which potential pathogens are killed (Lushchak, 2011;Gajbhiye and Khandeparker, 2017). But if the production of ROS exceeds the antioxidant capacity of bivalves, it will cause cell oxidative damage (Cheng et al, 2004;Gu et al, 2020), so that the reduction of THC may result from high ROS production (Wu et al, 2018b). In this study, THC at low salinity was indeed significantly reduced, and diel-cycling hypoxia also exacerbated the production of ROS in salinity changed water.…”

Section: Discussionmentioning

confidence: 53%

“…The circulating hemocytes of bivalves are susceptible to external factors, such as salinity fluctuations (Gajbhiye and Khandeparker, 2017), temperature changes (Gagnaire et al, 2006), reduced DO (Long et al, 2008), and organic pollutants (Gu et al, 2020). However, the combined effects of salinity change and DO reduction on marine species are limitedly studied nowadays rather than systematically and thoroughly (Wang et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Hemocyte Responses of the Oyster Crassostrea hongkongensis Exposed to Diel-Cycling Hypoxia and Salinity Change

et al. 2021

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Marine hypoxia caused by nutrient enrichment in coastal waters has become a global problem for decades, especially diel-cycling hypoxia that occurs frequently in the summer season. On the contrary, sudden rainstorms, and freshwater discharge make salinity in estuarine and coastal ecosystems variable, which often occurs with hypoxia. We found mass mortality of the Hong Kong oyster Crassostrea hongkongensis in the field where hypoxia and salinity fluctuation co-occur in the summer season during the past several years. To investigate the effects of diel-cycling hypoxia and salinity changes on the hemocyte immune function of C. hongkongensis, oysters were exposed to a combined effect of two dissolved oxygen (DO) concentrations (24 h normal oxygen 6 mg/L, 12 h normal oxygen 6 mg/L, and 12 h hypoxia 2 mg/L) and three salinities (10, 25, and 35‰) for 14 days. Subsequently, all treatments were restored to constant normal oxygen (6 mg/L) and salinity under 25‰ for 3 days to study the recovery of hemocyte immune function from the combined stress. Hemocyte parameters were analyzed by flow cytometry, including hemocyte mortality (HM), total hemocyte count (THC), phagocytosis (PHA), esterase (EST) activity, reactive oxygen species (ROS), lysosomal content (LYSO), and mitochondrial number (MN). The experimental results showed that diel-cycling hypoxia and salinity changes have obvious interactive effects on various immune parameters. In detail, diel-cycling hypoxia and decreases in salinity led to increased HM, and low salinity caused heavier impacts. In addition, low salinity, and diel-cycling hypoxia also led to decreases in LYSO, EST, and THC, while the decrease of PHA only occurs in the early stage. On the contrary, ROS production increased significantly under low salinity and hypoxic conditions. After 3-day recovery, THC, PHA, EST, LYSO, and MN were basically restored to normal, while HM and ROS were still significantly affected by diel-cycling hypoxia and salinity change, indicating that the combined stress of diel-cycling hypoxia and salinity changes had latent effects on the immune function of C. hongkongensis. Our results highlight that diel-cycling hypoxia and salinity change may impair the health and survival of the Hong Kong oyster C. hongkongensis and may be the key factors for the mass mortality of this oyster in the field.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Hemocyte Responses of the Oyster Crassostrea hongkongensis Exposed to Diel-Cycling Hypoxia and Salinity Change

et al. 2021

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“…The same uptake and elimination proportion of microplastic by mussels and the same change trend with time have been observed under different concentrations Albentosa, 2019a, Rist et al, 2019b). However, the opposite result showed that the clearance rate of mussels was inhibited under high concentrations of microplastic (Harris and Carrington, 2019;Gu et al, 2020). In addition, Woods et al (2018) showed that the uptake rate of microfibers by mussels initially increased and then remained stable with increasing exposure concentration, which implied a saturation of the uptake rate.…”

Section: Environmental or Exposure Concentrationmentioning

confidence: 96%

Where are we? Towards an understanding of the selective accumulation of microplastics in mussels

Rotchell

et al. 2021

Environmental Pollution

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“…Excessive ROS can induce lipid peroxidation of cell membranes and change the structure and function of cells, thus harming the health of organisms. MDA is a symbolic product of antioxidant damage ( Gu et al, 2020 ). In this study, acidification exposure significantly increased the ROS content in juvenile horseshoe crab.…”

Section: Discussionmentioning

confidence: 99%

Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus

Liu

Xiong

et al. 2022

Front. Physiol.

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Anthropogenic elevation of atmospheric carbon dioxide (CO2) drives global-scale ocean acidification (OA), which has aroused widespread concern for marine ecosystem health. The tri-spine horseshoe crab (HSC) Tachypleus tridentatus has been facing the threat of population depletion for decades, and the effects of OA on the physiology and microbiology of its early life stage are unclear. In this study, the 1st instar HSC larvae were exposed to acidified seawater (pH 7.3, pH 8.1 as control) for 28 days to determine the effects of OA on their growth, molting, oxidative stress, and gut microbiota. Results showed that there were no significant differences in growth index and molting rate between OA group and control group, but the chitinase activity, β-NAGase activity, and ecdysone content in OA group were significantly lower than those of the control group. Compared to the control group, reactive oxygen species (ROS) and malondialdehyde (MDA) contents in OA group were significantly increased at the end of the experiment. Superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatase (AKP) activities increased first and then decreased, glutathione peroxidase (GPX) decreased first and then increased, and GST activity changed little during the experiment. According to the result of 16S rRNA sequencing of gut microbiota, microbial-mediated functions predicted by PICRUSt showed that “Hematopoietic cell lineage,” “Endocytosis,” “Staphylococcus aureus infection,” and “Shigellosis” pathways significantly increased in OA group. The above results indicate that OA had no significant effect on growth index and molting rate but interfered with the activity of chitinolytic enzymes and ecdysone expression of juvenile horseshoe crabs, and caused oxidative stress. In addition, OA had adverse effects on the immune defense function and intestinal health. The present study reveals the potential threat of OA to T. tridentatus population and lays a foundation for the further study of the physiological adaptation mechanism of juvenile horseshoe crabs to environmental change.

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Microplastics aggravate the adverse effects of BDE-47 on physiological and defense performance in mussels

Cited by 79 publications

References 64 publications

Hemocyte Responses of the Oyster Crassostrea hongkongensis Exposed to Diel-Cycling Hypoxia and Salinity Change

Hemocyte Responses of the Oyster Crassostrea hongkongensis Exposed to Diel-Cycling Hypoxia and Salinity Change

Where are we? Towards an understanding of the selective accumulation of microplastics in mussels

Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus

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