Microplastic particles cause intestinal damage and other adverse effects in zebrafish Danio rerio and nematode Caenorhabditis elegans

Lei, Lili; Wu, Siyu; Lu, Shibo; Liu, Mengting; Song, Yang; Fu, Zhenhuan; Shi, Huahong; Raley‐Susman, Kathleen M.; He, Defu

doi:10.1016/j.scitotenv.2017.11.103

Cited by 951 publications

(325 citation statements)

References 46 publications

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“…In addition to size, the shape of the microplastics is influential. Irregularly shaped microplastic can cause histopathological damages, as observed in the intestine of adult zebrafish (Duis and Coors 2016;Horton et al 2017;Lei et al 2018) and European sea bass .…”

Section: The Physical Aspect: Consequences Of Microplastic Uptakementioning

confidence: 99%

Understanding How Microplastics Affect Marine Biota on the Cellular Level Is Important for Assessing Ecosystem Function: A Review

Prinz

Korez

2019

YOUMARES 9 - The Oceans: Our Research, Our Future

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Plastic has become indispensable for human life. When plastic debris is discarded into waterways, these items can interact with organisms. Of particular concern are microscopic plastic particles (microplastics) which are subject to ingestion by several taxa. This review summarizes the results of cutting-edge research about the interactions between a range of aquatic species and microplastics, including effects on biota physiology and secondary ingestion. Uptake pathways via digestive or ventilatory systems are discussed, including (1) the physical penetration of microplastic particles into cellular structures, (2) leaching of chemical additives or adsorbed persistent organic pollutants (POPs), and (3) consequences of bacterial or viral microbiota contamination associated with microplastic ingestion. Following uptake, a number of individual-level effects have been observed, including reduction of feeding activities, reduced growth and reproduction through cellular modifications, and oxidative stress. Microplastic-associated effects on marine biota have become increasingly investigated with growing concerns regarding human health through trophic transfer. We argue that research on the cellular interactions with microplastics provide an understanding of their impact to the organisms' fitness and, therefore, its ability to sustain their functional role in the ecosystem. The review summarizes information from 236 scientific publications. Of those, only 4.6% extrapolate their research of microplastic intake on individual species to the impact on ecosystem functioning. We emphasize the need for risk evaluation from organismal effects to an ecosystem level to effectively evaluate the effect of microplastic pollution on marine environments. Further studies are encouraged to investigate sublethal effects in the context of environmentally relevant microplastic pollution conditions.

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Section: The Physical Aspect: Consequences Of Microplastic Uptakementioning

confidence: 99%

Understanding How Microplastics Affect Marine Biota on the Cellular Level Is Important for Assessing Ecosystem Function: A Review

Prinz

Korez

2019

YOUMARES 9 - The Oceans: Our Research, Our Future

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“…Toxicities of MPs are widely studied by in vivo. The toxic effects of MPs on mortality, growth inhibition, body size reduction, reproduction alterations, intestinal damage, oxidative stress, and endocrine disruption of aquatic animals have been investigated . Previous studies reported that MPs can induce physical damages to the gut of marine organisms due to their accumulation in the gut .…”

Section: Introductionmentioning

confidence: 99%

Effects of polystyrene microbeads on cytotoxicity and transcriptomic profiles in human Caco‐2 cells

Tian

et al. 2019

Environmental Toxicology

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Microplastics (MPs) pollution is a global paradigm that raises concern in relation to environment and human health. In order to investigate the molecular toxicity mechanisms of MPs, transcriptomic analyses were performed on in vitro Caco‐2 cell model. After observing that polystyrene microplastics (PS‐MPs) decreased cell viability in a dose‐dependent manner, the responsible genes and involved pathways that might make contribution to PS‐MBs‐induced toxicity to Caco‐2 cells were identified with Illumina RNA seq. A total of 442 genes including, 210 up‐regulated ones and 232 down‐regulated ones, showed differential expression after treatment by PS‐MPs with a concentration of 12.5 mg L−1 or 50.0 mg L−1 for 24 hours. Gene Ontology (GO) annotation enriched unigenes can be grouped into three separated clusters: cellular component (CC), biological process (BP), and molecular function (MF). The dominate pathways related to NF‐κB, MAPK signaling, cytokine‐cytokine receptor interaction, and toll‐like receptor were strongly influenced by PS‐MBs. These pathways are involved in modulating cell inflammatory and proliferation. The qPCR were applied to investigate the transcriptional level of five proliferation related genes (Ras, ERK, MER, CDK4, Cyclin D1) and four inflammation related genes (TRPV1, iNOS, IL‐1β, IL‐8), and the results were consistent with RNA‐seq data. This study has provided new insight into the understanding of the toxicity effects of PS‐MBs‐induced intestinal inflammatory diseases.

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“…Thus, the palmitic acid present in PSNP/ F-PA could be chemically attractive to daphnids, strongly inducing the ingestion of these nanoplastics. This would lead to a decrease in the intake of microalgal cells, leading to a fake food satiation and causing mechanical injuries to the gut walls, wileyonlinelibrary.com/ETC © 2019 SETAC among other possible adverse effects, as found in previous studies with other freshwater animals (Lei et al 2018).…”

Section: Acute and Chronic Toxicity Tests With D Magnamentioning

confidence: 89%

Toxicological Evaluation and Quantification of Ingested Metal‐Core Nanoplastic by Daphnia magna Through Fluorescence and Inductively Coupled Plasma‐Mass Spectrometric Methods

Vicentini

Nogueira

Melegari

et al. 2019

Enviro Toxic and Chemistry

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There are few studies on nanoplastic that propose quantification of the amount ingested combined with evaluation of the toxic effects on aquatic organisms. We propose 2 methods to quantify the amount of polystyrene nanoplastic (PSNP) ingested by Daphnia magna: fluorescence intensity, where a fluorescent monomer (F) is added to the PSNP and quantified through fluorescence light microscopy, and total aluminum quantification, where PSNP is synthesized with Al2O3 metal‐core nanoparticles and used for quantification of the nanoplastic ingested by the organism Daphnia magna using inductively coupled plasma‐mass spectrometry. In addition, the PSNP was functionalized with palmitic acid to simulate the environmental conditions leading to biological and chemical transformations. Acute and chronic toxicity tests were performed with fluorescent PSNP (PSNP/F) and palmitic acid‐functionalized PSNP/F (PSNP/F‐PA). The ingestion quantified was higher by factors of 2.8 and 3.0 for PSNP/F‐PA and 1.9 and 1.7 for PSNP/F applying the fluorescence intensity and total Al quantifying methods, respectively, when compared to PSNP. These results are consistent with the data obtained in the toxicity tests, which showed an approximately 3 times increase in the adverse effect of PSNP/F‐PA on the mobility and reproduction of the organisms. Thus, the strong inhibition of D. magna reproduction caused by PSNP/F‐PA in the chronic toxicity tests could be associated with a greater amount of this nanoplastic being ingested by the organisms. Environ Toxicol Chem 2019;38:2101–2110. © 2019 SETAC.

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Microplastic particles cause intestinal damage and other adverse effects in zebrafish Danio rerio and nematode Caenorhabditis elegans

Cited by 951 publications

References 46 publications

Understanding How Microplastics Affect Marine Biota on the Cellular Level Is Important for Assessing Ecosystem Function: A Review

Understanding How Microplastics Affect Marine Biota on the Cellular Level Is Important for Assessing Ecosystem Function: A Review

Effects of polystyrene microbeads on cytotoxicity and transcriptomic profiles in human Caco‐2 cells

Toxicological Evaluation and Quantification of Ingested Metal‐Core Nanoplastic by Daphnia magna Through Fluorescence and Inductively Coupled Plasma‐Mass Spectrometric Methods

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