Microphysiological stem cell models of the human heart

Arslan, Ulgu; Moruzzi, Alessia; Nowacka, Joanna; Mummery, Christine L.; Eckardt, Dominik; Loskill, Peter; Orlova, Valeria V.

doi:10.1016/j.mtbio.2022.100259

Cited by 9 publications

(6 citation statements)

References 160 publications

(184 reference statements)

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“…Several 3D microphysiological models of the human heart have been described in which cell-cell crosstalk improved human pluripotent stem cell derived CM (hPSC-CM) maturation. These systems have proven useful for modeling some types of cardiac disease and for drug screening (reviewed in Arslan et al., 2022 ). Some of these models incorporated vascular networks inside engineered tissues, but in general, they were not perfusable by fluid or blood (equivalents) ( Zhang et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Vascularized hiPSC-derived 3D cardiac microtissue on chip

et al. 2023

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Section: Introductionmentioning

confidence: 99%

Vascularized hiPSC-derived 3D cardiac microtissue on chip

et al. 2023

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“…Heart cell responses to drug-induced and microenvironmental changes can be roughly divided into structural and functional responses ( Arslan et al, 2022 ). While the structural responses can be examined with various microscopies, functional responses requires dedicated sensor technologies.…”

Section: Functional Measurementsmentioning

confidence: 99%

Building blocks of microphysiological system to model physiology and pathophysiology of human heart

et al. 2023

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Microphysiological systems (MPS) are drawing increasing interest from academia and from biomedical industry due to their improved capability to capture human physiology. MPS offer an advanced in vitro platform that can be used to study human organ and tissue level functions in health and in diseased states more accurately than traditional single cell cultures or even animal models. Key features in MPS include microenvironmental control and monitoring as well as high biological complexity of the target tissue. To reach these qualities, cross-disciplinary collaboration from multiple fields of science is required to build MPS. Here, we review different areas of expertise and describe essential building blocks of heart MPS including relevant cardiac cell types, supporting matrix, mechanical stimulation, functional measurements, and computational modelling. The review presents current methods in cardiac MPS and provides insights for future MPS development with improved recapitulation of human physiology.

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“…Given their relatively low resource requirements and fabrication steps, organoid and spheroid systems have been at the forefront of considerable effort towards developing a human-relevant model of vascularized myocardium. Current state-of-the-art cardiac organoid systems have been developed for applications such as drug screening, cardiogenesis, and disease modeling, all of which have important implications for our understanding of EC-CM interactions ( Arslan et al, 2022 ).…”

Section: Current Models Of Endothelial Dysfunction In Cardiac Develop...mentioning

confidence: 99%

Endothelial cell dysfunction in cardiac disease: driver or consequence?

Allbritton-King,

García-Cardeña

2023

Front. Cell Dev. Biol.

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The vascular endothelium is a multifunctional cellular system which directly influences blood components and cells within the vessel wall in a given tissue. Importantly, this cellular interface undergoes critical phenotypic changes in response to various biochemical and hemodynamic stimuli, driving several developmental and pathophysiological processes. Multiple studies have indicated a central role of the endothelium in the initiation, progression, and clinical outcomes of cardiac disease. In this review we synthesize the current understanding of endothelial function and dysfunction as mediators of the cardiomyocyte phenotype in the setting of distinct cardiac pathologies; outline existing in vivo and in vitro models where key features of endothelial cell dysfunction can be recapitulated; and discuss future directions for development of endothelium-targeted therapeutics for cardiac diseases with limited existing treatment options.

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Microphysiological stem cell models of the human heart

Cited by 9 publications

References 160 publications

Vascularized hiPSC-derived 3D cardiac microtissue on chip

Vascularized hiPSC-derived 3D cardiac microtissue on chip

Building blocks of microphysiological system to model physiology and pathophysiology of human heart

Endothelial cell dysfunction in cardiac disease: driver or consequence?

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