Microperfusion of Henle’s Loop in the Kidney of the Adrenalectomized Rat

Murayama, Yusuke; Suzuki, Atsushi; Tadokoro, M.; Sakai, Fuminori

doi:10.1254/jjp.18.518

Cited by 22 publications

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“…When tubule flow rate was held constant by microperfusion, Murayama et al (7) found that large acute doses of cortisol did not have a significant effect on solute reabsorption in the loop ofHenle. In contrast, in a more recent microperfusion study, dexamethasone increased sodium reabsorption in the loop of Henle; however, the physiological significance ofthis effect is questionable steroid was given chronically to adrenalectomized rats (5).…”

Section: Discussionmentioning

confidence: 99%

“…Addisonian patients and animals deficient in these hormones have a decreased ability to generate a maximally concentrated urine (1)(2)(3). Sodium reabsorption by the loop of Henle is decreased in adrenalectomized rats, which suggests that part of the defect in the ability to concentrate the urine results from a reduction in the single effect of the countercurrent multiplier in the thick ascending limb of Henle's loop (4)(5)(6)(7)(8). In addition, some but not all studies have shown that adrenalectomy reduces potassium reabsorption in the loop of Henle (6,9,10).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, some but not all studies have shown that adrenalectomy reduces potassium reabsorption in the loop of Henle (6,9,10). Attempts to assess the effects of selective mineralocorticoid and glucocorticoid replacement on sodium and potassium reabsorption by the loop of Henle in adrenalectomized animals have yielded contradictory results (4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

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Regulation by adrenal corticosteroids of sodium and potassium transport in loop of Henle and distal tubule of rat kidney.

Stanton

1986

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation by adrenal corticosteroids of sodium and potassium transport in loop of Henle and distal tubule of rat kidney.

Stanton

1986

J. Clin. Invest.

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“…The method of this experiment was almost same as that previously reported (1). A proximal tubule of anesthetized rat (Nembutal 30 mg/kg i.v.)…”

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Effects of Cortisol on the Henle’s Loop of the Adrenalectomized Rat’s Kidney

Sakai

Murayama

1970

Japanese Journal of Pharmacology

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The experiment to be reported is an attempt to investigate the mechanism of action of cortisol in the Henle's loop.Male albino rats weighing 170 to 270 g were adrenalectomized and given free access to 0.9% saline. Six to seven days after adrenalectomy, five milligram of cortisol (alchohol free) was injected subcutaenously for 2 days previously. Additional cortisol (5 mg) was injected subcutaneously 60 minutes prior to the start of the experiment.The method of this experiment was almost same as that previously reported (1). A proximal tubule of anesthetized rat (Nembutal 30 mg/kg i.v.) was punctured with a perfusion pipette and a sample was collected from the first part of the distal segment of the surface of the kidney. Ringer's solution (NaCl; 147 mM KCl 4.02 mM, CaC12i 2.7 mM) containing inulin (0.15-0.20%) and lissamine green 0.05%) was used as perfusate and perfusion rate was 19 nl/min. The osmolality of this solution was 302 mOsm/L. The osmolality, Na and inulin concentration of the samples were measured with a micro-osmometer, an ultramicroflamephoto meter (Erma Model 677) and micro-fluorophotometer (AMINCO) (2). These values were shown as a TF/p

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“…The loop of Henle was perfused according to the method of Cortney et al (8) as has previously described (9). The perfusion pipet was placed in a late segment of a proximal tubule and a collection was made from the early segment of the distal tubule of the same nephron.…”

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Effects of Aldosterone and Cortisol on Henle's Loop in the Adrenalectomized Rat's Kidney

Sakai

Murayama

1971

Jpn.J.Pharmacol

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It is generally accepted that urine is concentrated by the mammalian kidney through the operation of a countercurrent mechanism in the renal medulla and that active sodium transport out of the ascending limb of Henle's loop plays an important role in this mecha nism. In addition, it has been reported that in the adrenalectomized rat urine volume is decreased and the corticomedullary osmotic gradient lowered (1, 2).Experiments studying the effects of aldosterone on free water clearance (3) and the attainment of maximum urinary osmolality (4), have suggested that aldosterone may cause an increased rate of transport of sodium from the inside of the ascending limb of Henle's loop to the surrounding tissue. On the other hand, the action of glucocorticoids, particu larly their effects on the loop of Henle have not yet been precisely defined. Experiments dealing with free water reabsorption (T°H2O) indicated, that glucocorticoids may enhance sodium transport at the ascending limb (5, 6). Hierholzer's experiments (7) suggested that cortisol affects the water permeability of distal tubular segments. In the present experiments we have attempted, by microperfusion of Henle's loop in the kidneys of ad renalectomized rats, to clarify the mode of action of corticosteroids on these tubular seg ments. METHODSMale albino rats weighing 150-230g were adrenalectomized and given free access to 0.9% saline and a dry pellet diet. Intact rats were kept on tap water. The following groups of rats were studied (a) intact animals on controls, (b) adrenalectomized animals 18-24 hours, 4 days and 7-9 days after operation, (c) animals adrenalectomized 7-9 days previously and given 12.5 micrograms of d-aldosterone monoacetate (Aldocorten, Ciba) subcutaneously prior to anesthesia. A further dose of 3.1 micrograms was given intra venously when the kidney had been prepared for micropuncture, (d) animals adrenalecto mized 7-9 days previously and given a subcutaneous injection of 2.5 mg cortisol (alcohol free, Merck) for 2 days. In some animals of this group, 5 mg of cortisol was given daily for 3 days prior to micropuncture. (e) The final group of animals were given both a] dosterone and cortisol similarly to those of group c and d.

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Microperfusion of Henle’s Loop in the Kidney of the Adrenalectomized Rat

Cited by 22 publications

References 1 publication

Regulation by adrenal corticosteroids of sodium and potassium transport in loop of Henle and distal tubule of rat kidney.

Regulation by adrenal corticosteroids of sodium and potassium transport in loop of Henle and distal tubule of rat kidney.

Effects of Cortisol on the Henle’s Loop of the Adrenalectomized Rat’s Kidney

Effects of Aldosterone and Cortisol on Henle's Loop in the Adrenalectomized Rat's Kidney

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