Microperforation of the colon: animal model in rats to reproduce mucosal thermal damage

Lorenzo-Zúñiga, Vicente; Boix, Jaume; Moreno-de-Vega, Vicente; de-la-Ossa, Napoleón D.; Òdena, Gemma; Bartolı́, Ramón

doi:10.1016/j.jss.2014.01.039

Cited by 7 publications

(11 citation statements)

References 6 publications

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“…Conventional EMR technique with prior submucosal injection was not able to be done as a result of wall thickness . Lesions were created in the left colon, 6 cm to the anal margin, by Coagrasper Hemostatic Forceps (Olympus), with a power setting of forced coagulation 40 W during 4 s . This technique induces deep thermal damage in the acute phase (48 h), with development of CS and large mucosal defects in the late phase, 1 week after the injury.…”

Section: Methodsmentioning

confidence: 53%

“…Previous reports in clinical practice have demonstrated that clipping closure or sealing bowel defects with tissue adhesives have achieved promising results in the prevention of delayed perforation by maintaining high mechanical strength; despite this, the use of clips sometimes is complex or is limited to lesions smaller than 40 mm . Tissue‐shielding method also has the disadvantage of being time‐consuming .…”

Section: Discussionmentioning

confidence: 99%

“…Thermal injury was evaluated with a 1–4 scale: (i) mucosal necrosis; (ii) submucosal necrosis; (iii) transmural necrosis; and (iv) peritonitis and/or microperforation, in hematoxylin and eosin (HE) histological sections using a conventional microscope (Olympus).…”

Section: Methodsmentioning

confidence: 99%

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Endoscopic shielding technique with a newly developed hydrogel to prevent thermal injury in two experimental models

Lorenzo-Zúñiga

Boix

Vega

et al. 2017

Digestive Endoscopy

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Section: Methodsmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

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Endoscopic shielding technique with a newly developed hydrogel to prevent thermal injury in two experimental models

Lorenzo-Zúñiga

Boix

Vega

et al. 2017

Digestive Endoscopy

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“…The PLC approach requires the animal to be kept in the same orientation during sequential colonoscopy procedure. Because polyp size is a risk factor for bleeding and other adverse events, 15–19 lesions were size-matched as closely as possible.…”

Section: Methodsmentioning

confidence: 99%

Development of a murine colonoscopic polypectomy model (with videos)

Ertem

Dashwood

Rajendran

et al. 2016

Gastrointestinal Endoscopy

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Background Colonoscopy provides a means for screening and removal of colonic adenomas, preventing such lesions from progressing to late-stage carcinoma. No preclinical model currently exists that closely parallels the clinical scenario with respect to polyp resection and recovery post-endoscopy. Methods When we used the polyposis in rat colon (Pirc) model, a new polypectomy methodology was developed. A novel “PLC” classification system (Polyp Number/Location/Clockwise orientation) also was devised in order to accurately and reproducibly specify the location of each lesion within the colon. Results One week after surgery, injuries to the polypectomy site were confined to the submucosa, indicating that little or no damage occurred to the inner muscle layer of the colon. Polypectomy sites occasionally continued to show ulcer formation, whereas others exhibited tissue regeneration. A pilot study (n = 6 animals), involving a total of 37 polypectomies, confirmed that the new methodology could be applied using either air insufflation or water-assisted techniques, with either hot or cold snare. As a general observation, polyps tended to be more fully distended and less flattened against the colonic mucosa using the water-assisted protocol, increasing the technical ease of ensnaring and resecting lesions. The PLC system proved to be straightforward, and facilitated longitudinal studies by allowing the investigator to track each polypectomy site upon repeated examination. Conclusions The Pirc model was ideally suited to colonoscopy with polypectomy. Because the main cause of morbidity in the Pirc model is blockage of the colon, polypectomy can be used as a preventive strategy, and will likely facilitate long-term investigations of single agent and combination therapies with potential direct clinical relevance.

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“…Colonic lesions were performed in the left colon, at 6 cm to the anal margin by Coagrasper Haemostatic Forceps (Olympus, Tokyo), with a power setting of 40 W over 4 seconds 6 7 ( Fig. 3 ).…”

Section: Methodsmentioning

confidence: 99%

Efficacy of platelet-rich plasma as a shielding technique after endoscopic mucosal resection in rat and porcine models

et al. 2016

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Background and study aims: The aims were to assess the efficacy of endoscopic application of Platelet-rich plasma (PRP) to prevent delayed perforation and to induce mucosal healing after endoscopic resections. Patients and methods: Colonic induced lesions were performed in rats (n = 16) and pigs (n = 4). Animals were randomized to receive onto the lesions saline (control) or PRP. Animals underwent endoscopic follow-up. Thermal injury was assessed with a 1 – 4 scale: (1) mucosal necrosis; (2) submucosal necrosis; (3) muscularis propria necrosis; and (4) serosal necrosis Results: Saline treatment showed 50 % of mortality in rats (P = 0.02). Mean ulcerated area after 48 hours and 7 days was significantly smaller with PRP than with saline (0.27 ± 0.02 cm2 and 0.08 ± 0.01 cm2 vs. 0.56 ± 0.1 cm2 and 0.40 ± 0.06 cm2; P < 0.001). The incidence of thermal injury was significantly lower with PRP (1.25 ± 0.46) than in controls (2.25 ± 0.50); P = 0.006. The porcine model showed a trend toward higher mucosal restoration in animals treated with PRP than with saline at weeks 1 and 2 (Median area in cm2: 0.55 and 0.40 vs. 1.32 and 0.79) Conclusions: Application of PRP to colonic mucosal lesions showed strong healing properties in rat and porcine models.

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Microperforation of the colon: animal model in rats to reproduce mucosal thermal damage

Cited by 7 publications

References 6 publications

Endoscopic shielding technique with a newly developed hydrogel to prevent thermal injury in two experimental models

Endoscopic shielding technique with a newly developed hydrogel to prevent thermal injury in two experimental models

Development of a murine colonoscopic polypectomy model (with videos)

Efficacy of platelet-rich plasma as a shielding technique after endoscopic mucosal resection in rat and porcine models

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