Micropatterning of acoustic droplet vaporization in acoustically-responsive scaffolds using extrusion-based bioprinting

Aliabouzar, Mitra; Ley, Adam W.Y.; Meurs, Sabine; Putnam, Andrew J.; Baker, Brendon M.; Kripfgans, Oliver D.; Fowlkes, J. Brian; Fabiilli, Mario L.

doi:10.1016/j.bprint.2021.e00188

Cited by 9 publications

(4 citation statements)

References 63 publications

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“…We did not consider the size distribution of the droplets because they were administered by local interventional injection rather than intravenous injection. PFP droplets can be vaporized at the PNP thresholds of 1.05 MPa (2.25 MHz), 1.89 MPa (5 MHz), [33][34][35] meaning the lower the frequency, the lower the pressure threshold. At 596 kHz, the pressure threshold may be even lower than 1 MPa.…”

Section: Discussionmentioning

confidence: 99%

A new sonoablation using acoustic droplet vaporization and focused ultrasound: A feasibility study

Li,

He,

Tang

et al. 2023

Medical Physics

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BackgroundHistotripsy and boiling histotripsy are two methods of mechanical ablation that use high‐pressure focused ultrasound (FUS).PurposeHere, a new bubble sonoablation technique was investigated using low‐pressure FUS in combination with local injection of perfluoropentane (PFP) in rabbit liver.MethodsFifteen healthy New Zealand white rabbits were treated with FUS alone, FUS + PFP or PFP alone. FUS was performed using a single‐element focused transducer (frequency 596 kHz, 0.27 ms pulses, 0.54% duty cycle, and peak negative pressure 2.0 MPa). Ten minutes before FUS treatment, the PFP droplet was locally injected into the rabbit liver, where the ultrasound was focused. Contrast‐enhanced ultrasound (CEUS) of the liver was performed, and the temperature at the liver surface in the targeted liver region was recorded during treatment. The livers were collected for pathological examination. Statistical significance was set at p < 0.05. Paired t‐tests were used to compare the pre‐ and post‐treatment values. One‐way analysis of variance was performed to compare multiple groups, and the least significant difference method was used for further comparisons between the two groups.ResultsAnalysis of CEUS data showed that the values of area under the curve (AUC) were significantly different in the PFP + FUS group pre‐ (10453.644 ± 1182.93) and post‐treatment (4058.098 ± 2720.41), and the AUC values of PFP + FUS post‐treatment (4058.098 ± 2720.41) were also significantly lower than those of the FUS (9946.694 ± 1071.54) and the PFP (10364.794 ± 2181.53) groups. The peak intensity values also showed the same results, the value of peak intensity of PFP+FUS post‐treatment was 82.958 ± 13.99, whereas there was no difference between FUS (106.61 ± 7.61) and PFP (104.136 ± 10.55). Hematoxylin and eosin (H&E) staining revealed that the pathological damage ratings of the PFP + FUS, PFP, and FUS groups were grade 3, grade 1, and grade 0, respectively. Specifically, the area of liver necrosis in the PFP + FUS group (0.99 ± 0.29 cm2) was 198 times higher than that in the PFP group (0.005 ± 0.008 cm2), whereas no necrosis was observed in the livers treated with FUS alone. Simultaneously, the number of vacuoles in the liver of the PFP + FUS group (35.50 ± 23.31) was approximately five times that of the PFP group (7.00 ± 12.88), whereas no vacuoles were found in the liver treated with FUS alone.ConclusionPFP droplets combined with FUS can destroy liver tissue and cause tissue necrosis in the droplet injection area, without affecting the structure of surrounding tissue.

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Section: Discussionmentioning

confidence: 99%

A new sonoablation using acoustic droplet vaporization and focused ultrasound: A feasibility study

Li,

He,

Tang

et al. 2023

Medical Physics

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“…Monodisperse microdroplets were generated via a microfluidic-based chip (Cat# 3200146, Dolomite, Royston, United Kingdom). The details of production of phase-shift microdroplets can be found in our prior publications [29] , [30] . PFP (CAS# 355–42-0, Strem Chemicals), PFH (CAS# 355–42-0, Strem Chemicals) or PFO (CAS# 307–34–6, Sigma-Aldrich) was used as the perfluorocarbon phase.…”

Section: Methodsmentioning

confidence: 99%

“…Shear elastic and loss moduli of fibrin hydrogels were measured using an AR-G2 rheometer (TA instruments, New Castle, USA) with a parallel plate geometry (measurement head diameter: 8 mm, gap height: 2000 μm, temperature: 37 °C). Samples were then subjected to oscillatory shear at 1 Hz with a strain of 1 %, based on the linear viscoelastic regime determined previously [30] , to obtain shear elastic and loss moduli. The concentrations were chosen to mimic soft tissue properties [35] , [36] .…”

Section: Methodsmentioning

confidence: 99%

Ultra-high-speed dynamics of acoustic droplet vaporization in soft biomaterials: Effects of viscoelasticity, frequency, and bulk boiling point

Abeid,

Fabiilli,

Estrada

et al. 2024

Ultrasonics Sonochemistry

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“…A longer diffusion path is expected in acoustically responsive scaffolds with stable bubble formation (i.e., in the case of perfluorohexane) since released payloads cannot diffuse through a bubble, but rather must diffuse around it. More advanced techniques such as 3D bioprinting can be utilized to micropattern payload-carrying droplets as well as control the response of the generated bubbles in acoustically-responsive scaffolds [100] . The unique stable or transient bubble formation induced by ADV can be utilized in applications beyond tissue regeneration.…”

Section: Tuning Adv Dynamics In Tissue Regenerationmentioning

confidence: 99%

Bubble nucleation and dynamics in acoustic droplet vaporization: a review of concepts, applications, and new directions

Aliabouzar

Kripfgans

Fowlkes

et al. 2023

Zeitschrift für Medizinische Physik

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Micropatterning of acoustic droplet vaporization in acoustically-responsive scaffolds using extrusion-based bioprinting

Cited by 9 publications

References 63 publications

A new sonoablation using acoustic droplet vaporization and focused ultrasound: A feasibility study

A new sonoablation using acoustic droplet vaporization and focused ultrasound: A feasibility study

Ultra-high-speed dynamics of acoustic droplet vaporization in soft biomaterials: Effects of viscoelasticity, frequency, and bulk boiling point

Bubble nucleation and dynamics in acoustic droplet vaporization: a review of concepts, applications, and new directions

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