Micropatterning in BioMEMS for Separation of Cells/Bioparticles

Kumar, R. Harish; Panwala, Fenil C.

doi:10.5772/intechopen.76060

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…Bioseparation is crucial during lab-on-chip testing (LOCT) (Kumar and Panwala, 2018). Blood separation enables in-vivo health monitoring through on-chip detection (Su et al , 2020).…”

Section: Introductionmentioning

confidence: 99%

Rapid prototyping of 3d printed micropillars using fused filament fabrication technique for biomedical applications

Khatoon,

Ahmad

2023

RPJ

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Purpose The hygroscopic properties of 3D-printed filaments and moisture absorption itself during the process result in dimensional inaccuracy, particularly for nozzle movement along the x-axis and for micro-scale features. In view of that, this study aims to analyze in depth the dimensional errors and deviations of the fused filament fabrication (FFF)/fused deposition modeling (FDM) 3D-printed micropillars (MPs) from the reference values. A detailed analysis into the variability in printed dimensions below 1 mm in width without any deformations in the printed shape of the designed features, for challenging filaments like polymethyl methacrylate (PMMA) has been done. The study also explores whether the printed shape retains the designed structure. Design/methodology/approach A reference model for MPs of width 800 µm and height 2,000 µm is selected to generate a g-code model after pre-processing of slicing and meshing parameters for 3D printing of micro-scale structure with defined boundaries. Three SETs, SET-A, SET-B and SET-C, for nozzle diameter of 0.2 mm, 0.25 mm and 0.3 mm, respectively, have been prepared. The SETs containing the MPs were fabricated with the spacing (S) of 2,000 µm, 3,200 µm and 4,000 µm along the print head x-axis. The MPs were measured by taking three consecutive measurements (top, bottom and middle) for the width and one for the height. Findings The prominent highlight of this study is the successful FFF/FDM 3D printing of thin features (<1mm) without any deformation. The mathematical analysis of the variance of the optical microscopy measurements concluded that printed dimensions for micropillar widths did not vary significantly, retaining more than 65% of the recording within the first standard deviation (SD) (±1 s). The minimum value of SD is obtained from the samples of SET-B, that is, 31.96 µm and 35.865 µm, for height and width, respectively. The %RE for SET-B samples is 5.09% for S = 2,000µm, 3.86% for S = 3,200µm and 1.09% for S = 4,000µm. The error percentage is so small that it could be easily compensated by redesigning. Research limitations/implications The study does not cover other 3D printing techniques of additive manufacturing like stereolithography, digital light processing and material jetting. Practical implications The presented study can be potentially implemented for the rapid prototyping of microfluidics mixer, bioseparator and lab-on-chip devices, both for membrane-free bioseparation based on microfiltration, plasma extraction from whole blood, size-selection trapping of unwanted blood cells, and also for membrane-based plasma extraction that requires supporting microstructures. Our developed process may prove to be far more economical than the other existing techniques for such applications. Originality/value For the first time, this work presents a comprehensive analysis of the fabrication of micropillars using FDM/FFF 3D printing and PMMA in filament form. The primary focus of the study is to minimize the dimensional inaccuracies in the 3D printed devices containing thin features, especially in the area of biomedical engineering, by delivering benefits from the choice of the parameters. Thus, on the basis of errors and deviations, a thorough comparison of the three SETs of the fabricated micropillars has been done.

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“…Bioseparation is crucial during lab-on-chip testing (LOCT) (Kumar and Panwala, 2018). Blood separation enables in-vivo health monitoring through on-chip detection (Su et al , 2020).…”

Section: Introductionmentioning

confidence: 99%

Rapid prototyping of 3d printed micropillars using fused filament fabrication technique for biomedical applications

Khatoon,

Ahmad

2023

RPJ

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“…Figure 17. Cell separation mechanisms-geometry-based separation (a), affinity-based separation (b), optic separation (c), acoustic separation (d), electrokinetic separation (e).Reprinted from an open-access source[192].…”

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confidence: 99%

Microelectromechanical Systems (MEMS) for Biomedical Applications

Chircov

Grumezescu

2022

Micromachines

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The significant advancements within the electronics miniaturization field have shifted the scientific interest towards a new class of precision devices, namely microelectromechanical systems (MEMS). Specifically, MEMS refers to microscaled precision devices generally produced through micromachining techniques that combine mechanical and electrical components for fulfilling tasks normally carried out by macroscopic systems. Although their presence is found throughout all the aspects of daily life, recent years have witnessed countless research works involving the application of MEMS within the biomedical field, especially in drug synthesis and delivery, microsurgery, microtherapy, diagnostics and prevention, artificial organs, genome synthesis and sequencing, and cell manipulation and characterization. Their tremendous potential resides in the advantages offered by their reduced size, including ease of integration, lightweight, low power consumption, high resonance frequency, the possibility of integration with electrical or electronic circuits, reduced fabrication costs due to high mass production, and high accuracy, sensitivity, and throughput. In this context, this paper aims to provide an overview of MEMS technology by describing the main materials and fabrication techniques for manufacturing purposes and their most common biomedical applications, which have evolved in the past years.

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Micropatterning in BioMEMS for Separation of Cells/Bioparticles

Cited by 2 publications

References 24 publications

Rapid prototyping of 3d printed micropillars using fused filament fabrication technique for biomedical applications

Rapid prototyping of 3d printed micropillars using fused filament fabrication technique for biomedical applications

Microelectromechanical Systems (MEMS) for Biomedical Applications

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