Micropatterned substrates with physiological stiffness promote cell maturation and Pompe disease phenotype in human induced pluripotent stem cell‐derived skeletal myocytes

Jiwlawat, Nunnapas; Lynch, Eileen M.; Napiwocki, Brett N.; Stempien, Alana; Ashton, Randolph S.; Kamp, Timothy J.; Crone, Wendy C.; Suzuki, Masatoshi

doi:10.1002/bit.27075

Cited by 33 publications

(18 citation statements)

References 64 publications

(100 reference statements)

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“…The increase production of extracellular matrix components such as GAG is important for when trying to regenerate corneal stroma either by replacing a scaffold over time or by using a self-assembly approach, where the cells need to produce sufficient ECM in culture to form a functional tissue 44 . Micro-patterns on a substrate surface and substrate stiffness can induce cues to improve formation and alignment of multinucleated myotubes, similar to myofibers,that can exhibit spontaneous contractions along the pattern's longitudinal direction 45 .…”

Section: Discussionmentioning

confidence: 99%

Influence of micropatterned substrates on keratocyte phenotype

Bhattacharjee

Cavanagh

Ahearne

2020

Sci Rep

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Substrate topographic patterning is a powerful tool that can be used to manipulate cell shape and orientation. To gain a better understanding of the relationship between surface topography and keratocyte behavior, surface patterns consisting of linear aligned or orthogonally aligned microchannels were used. Photolithography and polymer molding techniques were used to fabricate micropatterns on the surface of polydimethylsiloxane (PDMS). Cells on linear aligned substrates were elongated and aligned in the channel direction, while cells on orthogonal substrates had a more spread morphology. Both linear and orthogonal topographies induced chromatin condensation and resulted in higher expressions of keratocyte specific genes and sulfated glycosaminoglycans (sGAG), compared with non-patterned substrates. However, despite differences in cell morphology and focal adhesions, many genes associated with a native keratocyte phenotype, such as keratocan and ALDH3A1, remain unchanged on the different patterned substrates. This information could be used to optimize substrates for keratocyte culture and to develop scaffolds for corneal regeneration.Located at the front of the eye, the cornea is the transparent outer component of the eye, responsible for focusing light into the eye and protecting the internal structure from external irritations 1 . The cornea comprises of five main layers: epithelium, Bowman's layer, stroma, Descemet's membrane and endothelium 2,3 . The stroma is the major structural and functional unit of cornea constituting 90% of the total corneal thickness. The collagen fibril structure in the stroma allows it to be transparent which is vital for preserving vision 4 .Aligned collagen nanofibrils in stromal extracellular matrix (ECM) (mainly collagen I and V 5-8 ) form layers arranged orthogonally to one another. Keratocytes, the major cellular component of stroma, are scattered within these layers. Corneal transparency is attributed to the highly ordered orthogonal distribution of fibril layers, spacing between collagen fibrils, modulation of the diameter of collagen fibril attained by proteoglycans (i.e. keratocan and lumican 9-12 ) and crystallin proteins located within the cells. Keratocytes are dendritic in nature with expanded cellular network and compact cell body, enabling them to construct a three-dimensional network of interconnected cells 13,14 . Upon injury the cells lose their dendritic shape and change phenotype becoming fibroblastic or myofibroblastic.Globally around 10 million suffer from vision impairment resulting from corneal injury 13 . Annually around 30,000-40,000 corneal graft replacements are performed within USA and Europe. While rejection rates are low (under 10%) 13 , for high-risk patients the rejection rate can increase to approximately 49% and continues to increase over the patient's life. The limited availability of transplantable donated corneas exacerbates this problem. Synthetic keratoprostheses have been used as an alternative of allografts, however there is a relatively hig...

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Section: Discussionmentioning

confidence: 99%

Influence of micropatterned substrates on keratocyte phenotype

Bhattacharjee

Cavanagh

Ahearne

2020

Sci Rep

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“…Alternatively, photolithography and/or soft lithography are also applied as micropatterning techniques to produce various surface topographies. In one study, the cultivation of human iPSC-derived myogenic progenitors on PDMS-based micropatterned substrate resulted in larger fiber diameters, more myogenin-positive nuclei, and increased nuclear fusion compared to the cells cultivated on non-patterned substrates [ 209 ]. Freeze-drying is another technique used for microstructured scaffolds in skeletal muscle tissue engineering.…”

Section: Scaffold Topographies For Improved Generation Of Muscle Tissuementioning

confidence: 99%

Current Strategies for the Regeneration of Skeletal Muscle Tissue

Alarçin

Bal‐Öztürk

Avcı

et al. 2021

IJMS

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Traumatic injuries, tumor resections, and degenerative diseases can damage skeletal muscle and lead to functional impairment and severe disability. Skeletal muscle regeneration is a complex process that depends on various cell types, signaling molecules, architectural cues, and physicochemical properties to be successful. To promote muscle repair and regeneration, various strategies for skeletal muscle tissue engineering have been developed in the last decades. However, there is still a high demand for the development of new methods and materials that promote skeletal muscle repair and functional regeneration to bring approaches closer to therapies in the clinic that structurally and functionally repair muscle. The combination of stem cells, biomaterials, and biomolecules is used to induce skeletal muscle regeneration. In this review, we provide an overview of different cell types used to treat skeletal muscle injury, highlight current strategies in biomaterial-based approaches, the importance of topography for the successful creation of functional striated muscle fibers, and discuss novel methods for muscle regeneration and challenges for their future clinical implementation.

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“…For example, PDMS stamps generated using the same photolithography techniques described above can be used to transfer ECM proteins onto a surface in a process known as microcontact printing ( Qin et al, 2010 ). This process has been used to prescribe myotube alignment on Petri dishes ( Bajaj et al, 2011 ), PDMS-coated surfaces ( Bettadapur et al, 2016 ; Jiwlawat et al, 2019 ; Nesmith et al, 2016 ; Palchesko et al, 2012 ; Sun et al, 2013 ) and PA hydrogels ( Li et al, 2008a ). Photolithography has also been used to selectively expose strips of a PA hydrogel to UV light, which activates only the exposed regions for collagen binding and thus myoblast adhesion ( Engler et al, 2004 ).…”

Section: Engineered In Vitro Models Of Neuromusculmentioning

confidence: 99%

Neuromuscular disease modeling on a chip

Santoso

McCain

2020

Disease Models &Amp; Mechanisms

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Organs-on-chips are broadly defined as microfabricated surfaces or devices designed to engineer cells into microscale tissues with native-like features and then extract physiologically relevant readouts at scale. Because they are generally compatible with patient-derived cells, these technologies can address many of the human relevance limitations of animal models. As a result, organs-on-chips have emerged as a promising new paradigm for patient-specific disease modeling and drug development. Because neuromuscular diseases span a broad range of rare conditions with diverse etiology and complex pathophysiology, they have been especially challenging to model in animals and thus are well suited for organ-on-chip approaches. In this Review, we first briefly summarize the challenges in neuromuscular disease modeling with animal models. Next, we describe a variety of existing organ-on-chip approaches for neuromuscular tissues, including a survey of cell sources for both muscle and nerve, and two- and three-dimensional neuromuscular tissue-engineering techniques. Although researchers have made tremendous advances in modeling neuromuscular diseases on a chip, the remaining challenges in cell sourcing, cell maturity, tissue assembly and readout capabilities limit their integration into the drug development pipeline today. However, as the field advances, models of healthy and diseased neuromuscular tissues on a chip, coupled with animal models, have vast potential as complementary tools for modeling multiple aspects of neuromuscular diseases and identifying new therapeutic strategies.

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Micropatterned substrates with physiological stiffness promote cell maturation and Pompe disease phenotype in human induced pluripotent stem cell‐derived skeletal myocytes

Cited by 33 publications

References 64 publications

Influence of micropatterned substrates on keratocyte phenotype

Influence of micropatterned substrates on keratocyte phenotype

Current Strategies for the Regeneration of Skeletal Muscle Tissue

Neuromuscular disease modeling on a chip

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