Micropatterned polymer substrates control alignment of proliferating Schwann cells to direct neuronal regeneration

Schmalenberg, Kristine E.; Uhrich, Kathryn E.

doi:10.1016/j.biomaterials.2004.04.046

Cited by 96 publications

(69 citation statements)

References 22 publications

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“…are a few patterns created using the microcontact printing method to mimic the basement membrane structures of nanometer-sized pores and ridges [26]. Microcontact printing also has advantageous over other patterning methods as it does not involve the usage of harsh solvents and high temperatures [27]. Nanoimprint lithography is capable of creating patterns of sub-10 nm features and uses very simple equipment with convenient processing steps [28].…”

Section: Nanostructure Fabrication Methodsmentioning

confidence: 99%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

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Stem cells are a promising alternative for cell therapy applications because of their self-renewing capability and potential to differentiate into a wide range of specialized cell lineages, including osteoblasts, chondrocytes, cardiomyocytes, and neuronal cells. Different kinds of stem cells are considered for cell-based tissue engineering approaches, of which bone-marrow-derived mesenchymal stem cells (BM-MSCs), adipose-derived stem cells (ADSCs) and embryonic stem cells (ESCs) are frequently utilized for advancement of new tissue engineering strategies. Nanostructures created by natural, synthetic, or composite polymeric biomaterials provide artificial templates of the extracellular matrix (ECM). They possess a high surface area to volume ratio, are porous with good mechanical properties, are sufficient to serve as a biomimetic platform to attract stem cells, cause differentiation, and provide functioning of the tissues. Nanoscale features such as fibers, pits, and grooves modulate cell behavior and might even stimulate the differentiation of stem cells to specific lineages; for example, the nanotopographical cues combined with chemical cues influence the neuronal induction of MSCs, resulting in high microtubule-associated protein expressions. Scaffolds can also be impregnated with several ligands for adhesion of receptors to the cells, or with other regulatory molecules and growth M.P. Prabhakaran (*) and J. Venugopal E3-05-12, Health Care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore e-mail: nnimpp@nus.edu.sg L. Ghasemi-Mobarakeh Islamic Azad University, Najafabad Branch, Isfahan, Iran D. Kai NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore G. Jin and S. Ramakrishna Department of Mechanical Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore factors for designed cell behavior or controlled cell differentiations. Different polymeric blends or fibers incorporated with nanoparticles (such as hydroxyapatite) in aligned or custom-made patterns can induce specific differentiation of stem cells for bone, cartilage, cardiac, nerve, and skin tissue regeneration. In this review, we will discuss the current state of the art and future perspectives on stem cells and their differentiation on nanoengineered substrates for advanced tissue regeneration.

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Section: Nanostructure Fabrication Methodsmentioning

confidence: 99%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

View full text Add to dashboard Cite

show abstract

“…In the analysis, data were classified into 18 groups as previously described. 1,24 Highly guided neurons were defined as neurons with orientation angles relative to the micropatterns within ±10°(h o ). 7 Higher frequency of neuron orientations between -10°a nd 10°indicated that more neurons were guided by micropatterns, whereas lesser frequencies of neuron orientations between -10°and 10°represent more random neuron growth.…”

Section: Orientation Of Neurons On Various Pattern Dimensionsmentioning

confidence: 99%

“…Multiple micropatterning techniques have been developed to fabricate micron-sized features on various surfaces. 1,20,24,29 Many studies focused on utilizing surfaces with micropatterned adhesive molecules to control neuron cell guidance, 18,23,24,33,34 but not to promote neurite outgrowth rates. This study focuses on four distinct components: neurite length, rate, number, and orientation.…”

Section: Introductionmentioning

confidence: 99%

“…A few studies describe how pattern dimensions influence neurons, but these studies typically focus on neurite guidance on patterns, not neurite outgrowth rates. 3,16,24 Microscale plasma-initiated patterning (lPIP) was used to produce striped patterns of biomacromolecules including bovine serum albumin, immunoglobulin G, and laminin on poly(methyl methacrylate) (PMMA) surfaces. 11,12 The polymer, PMMA, was chosen because its transparency and biocompatibility are appropriate for cell studies.…”

Section: Introductionmentioning

confidence: 99%

“…The pattern dimensions ranged from 10 and 20 lm (neuron body size) to larger dimensions (30-50 lm), keeping the spacings set at 40 lm to prevent neurite bridging between micropatterns. 3,6,24 Dissociated dorsal root ganglia (DRG) neurons were cultured on lamininstriped micropatterns and the outgrowth rate, length, number, and orientation were analyzed. Overall, we focused on neuronal response rather than axonal response as controlling cellular behavior appears to result mainly from the cell bodies, not the axons.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimal Micropattern Dimensions Enhance Neurite Outgrowth Rates, Lengths, and Orientations

Song

Uhrich

2007

Ann Biomed Eng

Self Cite

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Micropattern dimensions can significantly influence neurite outgrowth orientation, rate, and length. Laminin micropatterns of various widths from 10 to 50 microm at 10 microm intervals separated by 40 microm spaces were generated on poly(methyl methacrylate) surfaces using microscale plasma-initiated patterning (microPIP). Dissociated dorsal root ganglion (DRG) neurons were seeded on the micropatterned surfaces and cultured for 24 h in serum-free media. Neurite outgrowth numbers, lengths, rates, and orientations were measured on all micropatterned substrates. The results indicated that the dimension of the laminin pattern influenced the neurite outgrowth length, rate, and orientation, but not the numbers of neurite outgrowth. Neurons on more than 30 microm wide laminin pattern showed faster neurite outgrowth compared to other dimensions, and relatively low orientation at 50 microm pattern dimensions. Neurites at 40 microm laminin pattern widths demonstrated the fastest outgrowth rates and were highly oriented. The 40 microm laminin dimension is wide enough to provide sufficient laminin amounts for neuron growth and narrow enough to efficiently guide neurites. Based on these results, adhesive protein micropatterns of 40 microm dimensions are recommended when investigating DRG neurons.

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Tissue‐Engineered Peripheral Nerve

Leach

2006

Wiley Encyclopedia of Biomedical Engineering

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Peripheral nerves can be injured by a variety of mechanisms but can regenerate on their own if the injury is not extensive. Nerve grafts, the current standard of surgical care for repairing large nerve gaps, are successful, but they can be associated with several major drawbacks. To overcome these disadvantages, researchers are designing “off‐the‐shelf” nerve guide implants; thus, a multidisciplinary effort is underway to engineer nerve repair through a deeper understanding of the dynamic cell–cell and cell–environment interactions that occur during regeneration. This entry, which focuses on literature published in the last 5 years, first emphasizes new nerve guide designs that have been demonstrated to improve functional regeneration in vivo . The second section reviews studies that have deepened our understanding of in vitro and in vivo neural regenerative responses via new biomaterials technologies and imaging techniques. These critical advancements in peripheral nerve tissue engineering provide templates for future mechanistic studies and improved strategies to aid functional regeneration in vivo .

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Micropatterned polymer substrates control alignment of proliferating Schwann cells to direct neuronal regeneration

Cited by 96 publications

References 22 publications

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Optimal Micropattern Dimensions Enhance Neurite Outgrowth Rates, Lengths, and Orientations

Tissue‐Engineered Peripheral Nerve

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