Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc Receptors

Mohr, Jordan D; Ramezani, Meraj; Holowka, David; Baird, Barbara

doi:10.1007/978-1-0716-1944-5_1

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…Due to the relationship between innate and humoral immunity, as well as its importance in in ammation and infectious illnesses, FcγR has been the subject of a lot of research. Within the human FcγR family, FcγRII (active FcγRIIa and FcγRIIc, as well as inhibitory FcγRIIb) are unique in their ability to signal independently of the common γ chain (Patel and Bussel 2020; Mohr et al 2022). An Fc receptor, by de nition, is a receptor for the Fc component of an immunoglobulin (Ig).…”

Section: Discussionmentioning

confidence: 99%

Integrated single-cell and bulk RNA sequencing analysis revealing the role of FCGR2A on prognosis and immune infiltration of hepatocellular carcinoma

Tang

Bai

Chen

et al. 2022

Preprint

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Objective To assess potential prognostic biomarkers of hepatocellular carcinoma (HCC) and preliminarily investigate their immune-related functions.Methods The CIBERSORT and ESTIMATE computational methods were applied to analyze the tumor microenvironment (TME) using the transcriptome and clinical data of HCC from The Cancer Genome Atlas (TCGA) database. The target gene was screened by intersecting the PPI network with Univariate Cox Regression Analysis. The distribution of the target gene expression in tumor-infiltrating immune cells (TICs) and immunological function were explored. Quality control, principal component analysis, TSNE clustering analysis, and cell annotation were also performed after downloading the single-cell RNA-sequencing data from the NCBI-GEO database. Lastly, we analyzed the distribution of the target gene at the single-cell level and established a comprehensive network of immune-related functions.Results Of the five hub genes (MMP9, FCGR2A, TLR2, CCR1 and PLAUR), FCGR2A was identified as the target gene following intersection analysis. It was up-regulated in tumor tissues and associated with poor prognosis of HCC patients. TSNE clustering study yielded 35 clusters, with hepatocyte, T cell, NK cell, macrophage, monocyte, B cell, smooth muscle cell, and endothelial cell as the result of annotation. Two of the results were related to immunological function. CIBERSORT analysis for the proportion of TICs revealed an increase in eosinophil number in tissues with low expression of FCGR2A. At the single-cell level, FCGR2A was mainly distributed in macrophages and monocytes.Conclusion This study showed that FCGR2A had an immunological role in the mono-macrophage system, was a promising biomarker for poor survival and could be a novel therapeutic target for HCC.

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Section: Discussionmentioning

confidence: 99%

Integrated single-cell and bulk RNA sequencing analysis revealing the role of FCGR2A on prognosis and immune infiltration of hepatocellular carcinoma

Tang

Bai

Chen

et al. 2022

Preprint

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A Simplified and Robust Activation Procedure of Glass Surfaces for Printing Proteins and Subcellular Micropatterning Experiments

et al. 2022

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Depositing biomolecule micropatterns on solid substrates via microcontact printing (µCP) usually requires complex chemical substrate modifications to initially create reactive surface groups. Here, we present a simplified activation procedure for untreated solid substrates based on a commercial polymer metal ion coating (AnteoBindTM Biosensor reagent) that allows for direct µCP and the strong attachment of proteins via avidity binding. In proof-of-concept experiments, we identified the optimum working concentrations of the surface coating, characterized the specificity of protein binding and demonstrated the suitability of this approach by subcellular micropatterning experiments in living cells. Altogether, this method represents a significant enhancement and simplification of existing µCP procedures and further increases the accessibility of protein micropatterning for cell biological research questions.

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Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc Receptors

Cited by 2 publications

References 24 publications

Integrated single-cell and bulk RNA sequencing analysis revealing the role of FCGR2A on prognosis and immune infiltration of hepatocellular carcinoma

Integrated single-cell and bulk RNA sequencing analysis revealing the role of FCGR2A on prognosis and immune infiltration of hepatocellular carcinoma

A Simplified and Robust Activation Procedure of Glass Surfaces for Printing Proteins and Subcellular Micropatterning Experiments

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