Microparticles in stored canine <scp>RBC</scp> concentrates

Herring, Jennifer; Smith, Stephanie A.; McMichael, Maureen; O'Brien, Mauria; Ngwenyama, Thandeka R.; Corsi, Raffaella; Galligan, Alyssa; Beloshapka, Alison N.; Deng, Ping; Swanson, Kelly S.

doi:10.1111/vcp.12034

Cited by 47 publications

(63 citation statements)

References 33 publications

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“…Prestorage LR could mitigate development of RBC storage lesions. Microparticle formation and procoagulant phospholipid concentrations were similar between canine LR and non‐LR pRBC units when stored for up to 7 days . However, after longer storage periods, the levels of microparticles and procoagulant phospholipids were significantly increased in non‐LR versus LR pRBCs .…”

Section: Discussionmentioning

confidence: 90%

A pilot study evaluating the effects of prestorage leukoreduction on markers of inflammation in critically ill dogs receiving a blood transfusion

Lozano

Blois

Wood

et al. 2019

J Vet Emergen Crit Care

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Objectives To compare markers of inflammation after transfusion of leukoreduced (LR) packed RBCs (pRBCs) versus non‐LR pRBCs in dogs with critical illness requiring blood transfusion, and to report survival to discharge and rates of transfusion reactions in these dogs. Design Prospective randomized blinded clinical study June 2014–September 2015. Setting University veterinary teaching hospital. Animals Twenty‐three client‐owned critically ill dogs, consecutively enrolled. Interventions Dogs requiring a single pRBC transfusion were randomized into the LR or non‐LR pRBC group. Exclusion criteria included: requirement for multiple blood products, history of previous blood transfusion, and administration of anti‐inflammatory or immunosuppressive medication prior to enrollment. Measurements Blood samples were obtained immediately prior to transfusion, then 2 and 24 hours following transfusion. Parameters measured at each time point included: PCV, WBC count, segmented and band neutrophil counts, fibrinogen, and plasma lactate and C‐reactive protein concentrations. Acute patient physiologic and laboratory evaluation fast score was calculated on admission. Results Eleven dogs were included in the LR group and 12 in the non‐LR group; scores of illness severity were not significantly different between groups. Total WBC count was significantly higher in the non‐LR versus LR group 24 hours following pRBC transfusion, but this difference was not evident 2 hours following transfusion. No other inflammatory parameters at any time point were significantly different between LR versus non‐LR pRBC transfused dogs. Survival rates to discharge for LR and non‐LR groups were 8/11 and 9/12, respectively. Acute transfusion reactions were identified in 1/11 and 2/12 dogs in the LR and non‐LR group, respectively. All transfused blood was stored ≤12 days. Conclusions Most markers of inflammation did not significantly increase following transfusion of LR versus non‐LR pRBCs stored ≤12 days in ill dogs. Further prospective, randomized trials are needed in clinically ill dogs to determine the benefit of prestorage leukoreduction.

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Section: Discussionmentioning

confidence: 90%

A pilot study evaluating the effects of prestorage leukoreduction on markers of inflammation in critically ill dogs receiving a blood transfusion

Lozano

Blois

Wood

et al. 2019

J Vet Emergen Crit Care

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“…The Food and Drug Administration mandates that the maximal allowable shelf‐life of stored human RBCs requires maintenance of cellular integrity (ie, free hemoglobin <1% of total hemoglobin in the RBC unit) and adequate 24‐hour RBC recovery post‐transfusion (ie, on average, ≥75% of transfused RBCs must remain in the circulation). Although the specific mechanism or mechanisms by which transfusion of older, stored RBCs may increase morbidity and mortality is not yet known, potential mediators include the accumulation of microparticles in RBC units during storage, release of free hemoglobin, and increased plasma concentration of nontransferrin bound iron (NTBI) after rapid clearance of storage‐damaged RBCs by the reticuloendothelial system …”

mentioning

confidence: 99%

Effect of Duration of Packed Red Blood Cell Storage on Morbidity and Mortality in Dogs After Transfusion: 3,095 cases (2001–2010)

Hann

Brown

King

et al. 2014

Veterinary Internal Medicne

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BackgroundAccumulating evidence suggests that transfusion of packed red blood cells (PRBCs) stored for >14 days is associated with increased rates of sepsis, multiple organ dysfunction, and mortality in human patients.ObjectiveTo determine if duration of PRBC storage has an effect on morbidity and mortality in dogs after transfusion.AnimalsDogs admitted to the Matthew J Ryan Veterinary Hospital of the University of Pennsylvania.MethodsA retrospective case review of dogs identified through blood bank logbooks that received PRBC transfusions (minimum, 5 mL/kg) between 2001 and 2010. Dogs were categorized according to major cause of anemia (eg, hemorrhage, hemolysis, ineffective erythropoiesis) for analysis.ResultsA total of 3,095 dogs received 5,412 PRBC units. Longer duration of PRBC storage was associated with development of new or progressive coagulation failure (P = .001) and thromboembolic disease (P = .005). There was no association between duration of PRBC storage and survival for all dogs overall. However, a logistic regression model indicated that for dogs with hemolysis, 90% of which had immune‐mediated hemolytic anemia, longer duration of PRBC storage was a negative risk factor for survival. For every 7 day increase in storage, there was a 0.79 lesser odds of 30 day survival (95% CI, 0.64–0.97; P = .024).Conclusions and Clinical ImportanceDuration of PRBC storage does not appear to be a major contributing factor to mortality in the overall canine population. However, longer duration of PRBC storage may negatively impact outcome in dogs with immune‐mediated hemolytic anemia, thus warranting further investigation with prospective studies.

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“…[23][24][25][26] The benefits of leucoreduction in people include a reduction in febrile non-haemolytic transfusion reactions, 14,27-30 decreased incidence of transfusion-related acute lung injury, 31 decreased incidence of postoperative infections, 32 a reduction in the transmission of infectious diseases 33 and reduced mortality rate. Many studies have verified the ability of leucoreduction to reduce bioactive substances in human and canine transfusion products.…”

Section: Characteristicmentioning

confidence: 99%

Interleukin‐8, interleukin‐1β and tumour necrosis factor‐α in sequential units of packed red blood cells collected from retired racing Greyhounds

et al. 2017

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Background We hypothesised that concentrations of interleukin-8 (IL-8), interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) would increase during storage in the third sequential unit (U3) of canine packed red blood cells (PRBC) collected from terminal donors in haemorrhagic shock. We further hypothesised that leucoreduction would prevent cytokine accumulation in U3 and that cytokine concentrations in U3 would be higher than in the first units (U1) collected from the same dogs.Methods U1 and U3 were each collected from 12 anaesthetised healthy Greyhounds. Removal of leucocytes from half of each PRBC unit produced one leucoreduced (LR) and one nonleucoreduced (NLR) unit. Canine IL-8, IL-1β and TNF-α concentrations were measured in samples collected from the units during storage on days 0, 10, 20, 30 and 37. ResultsThe IL-8 concentration in U3 NLR units was significantly higher on days 10, 20, 30 and 37 than on day 0 and was significantly higher than in the LR units at all time points. The IL-1β concentration in U3 did not change over time, or between LR and NLR units. TNF-α was not detected in any unit. There were no significant differences in IL-8 or IL-1β concentrations between U3 and U1 at any time point; however, some NLR U3 units had markedly elevated IL-8 concentrations at day 37 (2060-20,682 pg/mL) compared with NLR U1 units (3369-5280 pg/mL).Conclusion NLR U3 units collected from dogs in haemorrhagic shock showed a significant increase in IL-8 concentrations during storage. Leucoreduction was effective at preventing the accumulation of IL-8. There was no difference detected between U3 and U1.

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Microparticles in stored canine RBC concentrates

Abstract: There was marked formation of phosphatidylserine-expressing MPs during storage beyond 7 days in canine RBC concentrates. Prestorage LR attenuated the generation of MPs.

Cited by 47 publications

References 33 publications

A pilot study evaluating the effects of prestorage leukoreduction on markers of inflammation in critically ill dogs receiving a blood transfusion

A pilot study evaluating the effects of prestorage leukoreduction on markers of inflammation in critically ill dogs receiving a blood transfusion

Effect of Duration of Packed Red Blood Cell Storage on Morbidity and Mortality in Dogs After Transfusion: 3,095 cases (2001–2010)

Interleukin‐8, interleukin‐1β and tumour necrosis factor‐α in sequential units of packed red blood cells collected from retired racing Greyhounds

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