Microparticles in newborn cord blood: Slight elevation after normal delivery

Schweintzger, Sabrina; Schlagenhauf, Axel; Leschnik, B.; Rinner, Beate; Bernhard, Heike; Novak, M. John; Muntean, W.

doi:10.1016/j.thromres.2011.01.013

Cited by 22 publications

(17 citation statements)

References 47 publications

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“…Thrombin generation was performed using Calibrated Automated Thrombography according to Hemker et al . as reported previously 20,27 . This method allows the continuous tracing of thrombin generated in-vitro in a specific plasma sample over time.…”

Section: Methodssupporting

confidence: 86%

“…Possibly, lower amounts of negatively charged phospholipids are required to optimally support thrombin generation in newborns due to lower plasma levels of tissue factor pathway inhibitor (TFPI) and antithrombin (AT) in the neonatal haemostatic system. Additionally, elevated microparticles in CB may contribute to the overall phospholipid content to provide sufficient support of thrombin generation 27 . We tested this hypothesis by mimicking neonatal and adult TFPI levels using TFPI depleted standard plasma and recombinant TFPI.…”

Section: Discussionmentioning

confidence: 99%

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Neonatal thrombocytopenia: Thrombin generation in presence of reduced platelet counts and effects of rFVIIa in cord blood

Haidl

Pohl

Leschnik

et al. 2019

Sci Rep

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Healthy neonates exhibit a well-functioning haemostatic system despite peculiarities regarding composition of clotting factors and inhibitors as well as impaired platelet aggregation. Thrombocytopenia and severe bleeding events are feared in sick infants. Recombinant factor VIIa (rFVIIa) is a haemostatic agent used as a last resort in neonates with refractory bleedings. Aim of this study was to investigate in-vitro (i) changes in thrombin generation with different platelet counts, (ii) effects of rFVIIa under conditions of thrombocytopenia and (iii) potentially differing dose-response of rFVIIa in cord blood as a surrogate for neonatal blood compared to adult blood. Thrombin generation parameters were observed in cord blood plasma and adult plasma with various platelet counts, with or without addition of rFVIIa, respectively. Low platelet counts did not influence thrombin generation in cord blood in contrast to adult blood. RFVIIa primarily affected lag time throughout all platelet concentrations. Interestingly, peak height was reduced exclusively in cord blood plasma after addition of rFVIIa. No significant differences regarding dose-response were observed between cord blood and adult blood. In contrast to adult blood, thrombocytopenia in cord blood does not significantly influence thrombin generation. Even at very low platelet counts there is enough negatively charged surface to support rFVIIa action in plasma from cord blood and adult blood in-vitro .

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Section: Methodssupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Neonatal thrombocytopenia: Thrombin generation in presence of reduced platelet counts and effects of rFVIIa in cord blood

Haidl

Pohl

Leschnik

et al. 2019

Sci Rep

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“…Maternal blood samples used in this study were collected shortly after birth, and it is therefore reasonable to assume that the blood profile is reflective of pregnancy. MP concentration in newborn cord blood has been found comparable with MP concentration in adult (non-pregnant) plasma samples [35].…”

Section: Plasma Quality Controlsupporting

confidence: 55%

MiRNA profiles in blood plasma from mother-child duos in human biobanks and the implication of sample quality: Circulating miRNAs as potential early markers of child health

et al. 2020

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BackgroundMicroRNAs (miRNAs) have been linked to several diseases and to regulation of almost every biological process. This together with their stability while freely circulating in blood suggests that they could serve as minimal-invasive biomarkers for a wide range of diseases. Successful miRNA-based biomarker discovery in plasma is dependent on controlling sources of preanalytical variation, such as cellular contamination and hemolysis, as they can be major causes of altered miRNA expression levels. Analysis of plasma quality is therefore a crucial step for the best output when searching for novel miRNA biomarkers. MethodsPlasma quality was assessed by three different methods in samples from mother-child duos (maternal and cord blood, N = 2x38), with collection and storage methods comparable to large cohort study biobanks. Total RNA was isolated and the expression profiles of 201 miR-NAs was obtained by qPCR to identify differentially expressed miRNAs in cord and maternal plasma samples. ConclusionsOur findings suggest that good quality plasma samples suitable for miRNA profiling can be achieved from samples collected and stored by large biobanks. Incorporation of extensive quality control measures, such as those established here, would be beneficial for future projects. The overall low correlation of miRNA expression between cord and maternal samples is an interesting observation, and promising for our future studies on identification of miRNA-based biomarkers in cord blood plasma, considering that these samples were collected at term and some exchange of blood components between cord and maternal blood frequently occur. IntroductionThe finding of circulating microRNAs (miRNA) in body fluids (blood plasma, serum or urine) [1,2] has opened up for the possibility of using blood-based miRNAs as a new approach for diagnostic screening. MiRNAs have been linked to cancer and several other diseases [3], and they have been implicated in promoting neurodevelopmental processes, such as neurogenesis, synapse precursor formation, and synaptic plasticity [4,5]. The stability of miRNAs circulating in blood, and their role as key regulators of almost every biological process, including precise control of neuronal gene expression and thus fine-tuning signaling pathways [6], suggests that they could serve as non-invasive biomarkers for a wide range of diseases [7][8][9], and possibly help explain basic disease etiology. Furthermore, miRNAs have been shown to be targeted by epigenetic modification, and in turn, miRNAs can target regulators of epigenetic pathways [10,11].Blood-based biobanks, which include collection of umbilical cord blood, such as the Mother, Father and Child Cohort Study (MoBa) biobank [12] at the Norwegian Institute of Public Health (NIPH), and other multi-center studies, increasingly incorporate studies identifying candidate mRNA and miRNA expression profile-based biomarkers for a wide range of disorders. The combination of biological specimens and questionnaire data on lifestyle and exposures in MoBa prov...

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“…Guzzetta and Downey summarize multiple signal transduction impairments in neonatal platelets that contribute to decreased platelet aggregation with several agonists. Still, according to our findings, phosphatidylserine expression and microparticle generation are not different from that of adult platelets, resulting in equal support of thrombin generation 7,8 . However, the authors mention improved platelet adhesion due to higher activity of neonatal von Willebrand factor (VWF).…”

mentioning

confidence: 48%

Contact activation and acquired von Willebrand syndrome during neonatal extracorporeal circulation

Schlagenhauf

Zieger

Muntean

2020

Journal of Thrombosis and Haemostasis

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itive aPL) 7 and that aPS/PT more than aβ2GPI is responsible for LA activity in these patients. 8 Finally, if aCL and aβ2GPI antibodies are of immunoglobulin G (IgG) isotype I would measure IgG aβ2GPI/domain 1 antibodies that are associated to thromboembolic events. 9 A tentative decision-making scheme to choose the appropriate anticoagulant treatment in young patients with venous thromboembolism is presented in Figure 1.

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Microparticles in newborn cord blood: Slight elevation after normal delivery

Cited by 22 publications

References 47 publications

Neonatal thrombocytopenia: Thrombin generation in presence of reduced platelet counts and effects of rFVIIa in cord blood

Neonatal thrombocytopenia: Thrombin generation in presence of reduced platelet counts and effects of rFVIIa in cord blood

MiRNA profiles in blood plasma from mother-child duos in human biobanks and the implication of sample quality: Circulating miRNAs as potential early markers of child health

Contact activation and acquired von Willebrand syndrome during neonatal extracorporeal circulation

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